The impact of nitrogen deposition on nitrogen metabolism in ryegrass lawn with different soil nutrient levels

Abstract Nitrogen deposition is a crucial factor in global change, which is widespread across various regions globally. It has drawn extensive attention due to its direct modification of soil nitrogen retention and nitrogen species distribution, thereby influencing nitrogen metabolism across entire...

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Main Authors: Xiu-Lin Song, Zi-Jie Wang, Xian-Wei Yin, Yan-Lin Sun, Dong-Jin Jang, Soon-Kwan Hong
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-00578-2
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author Xiu-Lin Song
Zi-Jie Wang
Xian-Wei Yin
Yan-Lin Sun
Dong-Jin Jang
Soon-Kwan Hong
author_facet Xiu-Lin Song
Zi-Jie Wang
Xian-Wei Yin
Yan-Lin Sun
Dong-Jin Jang
Soon-Kwan Hong
author_sort Xiu-Lin Song
collection DOAJ
description Abstract Nitrogen deposition is a crucial factor in global change, which is widespread across various regions globally. It has drawn extensive attention due to its direct modification of soil nitrogen retention and nitrogen species distribution, thereby influencing nitrogen metabolism across entire ecosystems. Previous studies on its influence on nitrogen metabolism have not reached a consensus. In an urban ryegrass lawn mesocosm experiment, we set two levels of nitrogen deposition and soil nutrients respectively, aiming to study the impacts of these factors on the N-cycling process through metagenomic analysis. The results demonstrated nitrogen deposition increased nitrification, nitrogen fixation, denitrification, and dissimilatory nitrate reduction, but decreased assimilatory nitrate reduction in the nitrogen metabolism process by changing soil nitrogen availability and the abundance of N-cycling functional genes in the soil microbial community. The soil nutrient levels exhibited effects opposite to those of nitrogen deposition, negatively impacting nitrification, denitrification, and nitrogen fixation in the nitrogen metabolism process. This work further elucidates the impacts of nitrogen deposition on the ecological functions of the ryegrass lawn with different soil nutrient levels, and predicts the potential impacts of intensified nitrogen deposition on these ecological functions. It provides valuable theoretical support for understanding and evaluating complex ecological interactions.
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spelling doaj-art-bba79479fdcb44999d0ef28734edfb262025-08-20T01:51:31ZengNature PortfolioScientific Reports2045-23222025-05-0115111610.1038/s41598-025-00578-2The impact of nitrogen deposition on nitrogen metabolism in ryegrass lawn with different soil nutrient levelsXiu-Lin Song0Zi-Jie Wang1Xian-Wei Yin2Yan-Lin Sun3Dong-Jin Jang4Soon-Kwan Hong5School of Life Sciences, Ludong UniversitySchool of Life Sciences, Ludong UniversitySchool of Life Sciences, Ludong UniversitySchool of Life Sciences, Ludong UniversitySchool of Life Sciences, Ludong UniversitySchool of Life Sciences, Ludong UniversityAbstract Nitrogen deposition is a crucial factor in global change, which is widespread across various regions globally. It has drawn extensive attention due to its direct modification of soil nitrogen retention and nitrogen species distribution, thereby influencing nitrogen metabolism across entire ecosystems. Previous studies on its influence on nitrogen metabolism have not reached a consensus. In an urban ryegrass lawn mesocosm experiment, we set two levels of nitrogen deposition and soil nutrients respectively, aiming to study the impacts of these factors on the N-cycling process through metagenomic analysis. The results demonstrated nitrogen deposition increased nitrification, nitrogen fixation, denitrification, and dissimilatory nitrate reduction, but decreased assimilatory nitrate reduction in the nitrogen metabolism process by changing soil nitrogen availability and the abundance of N-cycling functional genes in the soil microbial community. The soil nutrient levels exhibited effects opposite to those of nitrogen deposition, negatively impacting nitrification, denitrification, and nitrogen fixation in the nitrogen metabolism process. This work further elucidates the impacts of nitrogen deposition on the ecological functions of the ryegrass lawn with different soil nutrient levels, and predicts the potential impacts of intensified nitrogen deposition on these ecological functions. It provides valuable theoretical support for understanding and evaluating complex ecological interactions.https://doi.org/10.1038/s41598-025-00578-2Nitrogen depositionNitrogen metabolismSoil nutrient levelN-cycling functional genes
spellingShingle Xiu-Lin Song
Zi-Jie Wang
Xian-Wei Yin
Yan-Lin Sun
Dong-Jin Jang
Soon-Kwan Hong
The impact of nitrogen deposition on nitrogen metabolism in ryegrass lawn with different soil nutrient levels
Scientific Reports
Nitrogen deposition
Nitrogen metabolism
Soil nutrient level
N-cycling functional genes
title The impact of nitrogen deposition on nitrogen metabolism in ryegrass lawn with different soil nutrient levels
title_full The impact of nitrogen deposition on nitrogen metabolism in ryegrass lawn with different soil nutrient levels
title_fullStr The impact of nitrogen deposition on nitrogen metabolism in ryegrass lawn with different soil nutrient levels
title_full_unstemmed The impact of nitrogen deposition on nitrogen metabolism in ryegrass lawn with different soil nutrient levels
title_short The impact of nitrogen deposition on nitrogen metabolism in ryegrass lawn with different soil nutrient levels
title_sort impact of nitrogen deposition on nitrogen metabolism in ryegrass lawn with different soil nutrient levels
topic Nitrogen deposition
Nitrogen metabolism
Soil nutrient level
N-cycling functional genes
url https://doi.org/10.1038/s41598-025-00578-2
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