Elevated Proportions of Circulating CXCR5+ Follicular Helper T Cells Reflect the Presence of Airway Obstruction in Asthma
Rationale. The progressive decline in lung function observed in asthma is attributed to persistent type 2 airway inflammation and associated airway remodeling. While various immune cells are involved in localized type 2 airway inflammation, whether these reflect the presence of airway obstruction du...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2024/2020514 |
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| author | Tsukie Kin Tsukuda Kimiko Tsuji Akari Nishimori Takehiko Ito Yuka Kobayashi Taro Suzuki Akihito Yokoyama |
| author_facet | Tsukie Kin Tsukuda Kimiko Tsuji Akari Nishimori Takehiko Ito Yuka Kobayashi Taro Suzuki Akihito Yokoyama |
| author_sort | Tsukie Kin Tsukuda |
| collection | DOAJ |
| description | Rationale. The progressive decline in lung function observed in asthma is attributed to persistent type 2 airway inflammation and associated airway remodeling. While various immune cells are involved in localized type 2 airway inflammation, whether these reflect the presence of airway obstruction due to persistent type 2 airway inflammation remains unclear. This study aimed to explore whether the proportions of immune cells in peripheral blood indicate the presence of type 2 inflammation and airway obstruction in asthmatics. Materials and Methods. Using flow cytometry, we identified and quantified Group 2 innate lymphocytes, T helper 2 cells, follicular helper T cells, and T helper 17 cells in peripheral blood samples from 49 individuals with asthma. We then conducted cross-sectional analyses to assess relationships between levels of these immune cells and lung function parameters, including the percentage predicted forced expiratory volume in 1 s (%FEV1). We also examined correlations between the proportions of immune cells and type 2 biomarkers. Results. Proportions of CXCR5+ follicular helper T cells in human peripheral blood, as opposed to Group 2 innate lymphoid cells (ILC2) or T helper 2 cells, were significantly higher in cases with %FEV1 < 80% compared to those with %FEV1 ≥ 80%. Further, these proportions correlated negatively with %FEV1 and positively with blood eosinophil counts. Conclusions. The proportion of circulating follicular helper T cells, but not T helper 2 cells or Group 2 innate lymphoid cells, may reflect the presence of airway obstruction caused by persistent type 2 inflammation. |
| format | Article |
| id | doaj-art-bb985bbd242d43c88ee60d343d986214 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-bb985bbd242d43c88ee60d343d9862142025-02-03T00:22:16ZengWileyJournal of Immunology Research2314-71562024-01-01202410.1155/2024/2020514Elevated Proportions of Circulating CXCR5+ Follicular Helper T Cells Reflect the Presence of Airway Obstruction in AsthmaTsukie Kin Tsukuda0Kimiko Tsuji1Akari Nishimori2Takehiko Ito3Yuka Kobayashi4Taro Suzuki5Akihito Yokoyama6Department of Respiratory Medicine and AllergologyDepartment of Respiratory Medicine and AllergologyDepartment of Respiratory Medicine and AllergologyDepartment of Respiratory Medicine and AllergologyDepartment of Respiratory Medicine and AllergologyDepartment of Respiratory Medicine and AllergologyDepartment of Respiratory Medicine and AllergologyRationale. The progressive decline in lung function observed in asthma is attributed to persistent type 2 airway inflammation and associated airway remodeling. While various immune cells are involved in localized type 2 airway inflammation, whether these reflect the presence of airway obstruction due to persistent type 2 airway inflammation remains unclear. This study aimed to explore whether the proportions of immune cells in peripheral blood indicate the presence of type 2 inflammation and airway obstruction in asthmatics. Materials and Methods. Using flow cytometry, we identified and quantified Group 2 innate lymphocytes, T helper 2 cells, follicular helper T cells, and T helper 17 cells in peripheral blood samples from 49 individuals with asthma. We then conducted cross-sectional analyses to assess relationships between levels of these immune cells and lung function parameters, including the percentage predicted forced expiratory volume in 1 s (%FEV1). We also examined correlations between the proportions of immune cells and type 2 biomarkers. Results. Proportions of CXCR5+ follicular helper T cells in human peripheral blood, as opposed to Group 2 innate lymphoid cells (ILC2) or T helper 2 cells, were significantly higher in cases with %FEV1 < 80% compared to those with %FEV1 ≥ 80%. Further, these proportions correlated negatively with %FEV1 and positively with blood eosinophil counts. Conclusions. The proportion of circulating follicular helper T cells, but not T helper 2 cells or Group 2 innate lymphoid cells, may reflect the presence of airway obstruction caused by persistent type 2 inflammation.http://dx.doi.org/10.1155/2024/2020514 |
| spellingShingle | Tsukie Kin Tsukuda Kimiko Tsuji Akari Nishimori Takehiko Ito Yuka Kobayashi Taro Suzuki Akihito Yokoyama Elevated Proportions of Circulating CXCR5+ Follicular Helper T Cells Reflect the Presence of Airway Obstruction in Asthma Journal of Immunology Research |
| title | Elevated Proportions of Circulating CXCR5+ Follicular Helper T Cells Reflect the Presence of Airway Obstruction in Asthma |
| title_full | Elevated Proportions of Circulating CXCR5+ Follicular Helper T Cells Reflect the Presence of Airway Obstruction in Asthma |
| title_fullStr | Elevated Proportions of Circulating CXCR5+ Follicular Helper T Cells Reflect the Presence of Airway Obstruction in Asthma |
| title_full_unstemmed | Elevated Proportions of Circulating CXCR5+ Follicular Helper T Cells Reflect the Presence of Airway Obstruction in Asthma |
| title_short | Elevated Proportions of Circulating CXCR5+ Follicular Helper T Cells Reflect the Presence of Airway Obstruction in Asthma |
| title_sort | elevated proportions of circulating cxcr5 follicular helper t cells reflect the presence of airway obstruction in asthma |
| url | http://dx.doi.org/10.1155/2024/2020514 |
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