Serum level of Interleukin (IL)-8, Monocyte Chemoattractant Protein (MCP)-1 and Tumor Necrosis Factor (TNF)-α in children infected with Entamoeba histolytica
Entamoeba histolytica is an enteric protozoan parasite which caused tissue invasive amoebiasis. It can induce the production of cytokines (such as IL-8 and TNF-α) and chemokines (such as MCP-1) from intestinal epithelial cells. This study was done to detect the serum level of IL-8, MCP-1 and TNF-α,...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tikrit University
2019-08-01
|
| Series: | Tikrit Journal of Pure Science |
| Subjects: | |
| Online Access: | https://tjpsj.org/index.php/tjps/article/view/393 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849470320226861056 |
|---|---|
| author | Huda Muneer Ahmed Fatima Shihab Al-Nasiri |
| author_facet | Huda Muneer Ahmed Fatima Shihab Al-Nasiri |
| author_sort | Huda Muneer Ahmed |
| collection | DOAJ |
| description |
Entamoeba histolytica is an enteric protozoan parasite which caused tissue invasive amoebiasis. It can induce the production of cytokines (such as IL-8 and TNF-α) and chemokines (such as MCP-1) from intestinal epithelial cells. This study was done to detect the serum level of IL-8, MCP-1 and TNF-α, using an enzyme-linked immunosorbent assay, in 60 children affected by amoebiasis to correlate the production of these cytokines with infection and comparison with the healthy persons (n= 30). Three aged groups (1 month- 2 year, 2-4 year and 4-6 year) were entered in the study for patients and healthy controls. The level of IL-8 (37.22 ± 7.06, 37.35 ± 1.981 and 36.45 ± 4.39 pg/ml) in children with amoebiasis were higher significantly compared with the level in healthy children (29.95 ± 4.30, 26.90 ± 4.17 and 25.32 ± 2.31 pg/ml) in all age groups (1 month- 2 year, 2-4 year and 4-6 year, respectively). Significant differences in the level of MCP-1 which were higher (28.92 ± 1.48, 30.85 ± 3.78 and 31.91 ± 6.82 pg/ml) in children with amoebiasis than in healthy children (18.44 ± 0.74, 22.62 ± 6.25 and 18.30 ± 1.43 pg/ml) in all age groups (respectively). Also, the level of TNF-α were higher significantly (58.05 ± 6.90, 55.87 ± 3.81 and 57.32 ± 8.86 pg/ml) in children with amoebiasis compared with healthy children (42.91 ± 2.54, 43.54 ± 3.42 and 42.64 ± 3.47 pg/ml, respectively) in all age groups (respectively). These findings reflect a role of IL-8, MCP-1 and TNF-α in E. histolytica infection.
|
| format | Article |
| id | doaj-art-bb92efbd3d0147a6a1a0eb94184da165 |
| institution | Kabale University |
| issn | 1813-1662 2415-1726 |
| language | English |
| publishDate | 2019-08-01 |
| publisher | Tikrit University |
| record_format | Article |
| series | Tikrit Journal of Pure Science |
| spelling | doaj-art-bb92efbd3d0147a6a1a0eb94184da1652025-08-20T03:25:11ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262019-08-0124410.25130/tjps.v24i4.393Serum level of Interleukin (IL)-8, Monocyte Chemoattractant Protein (MCP)-1 and Tumor Necrosis Factor (TNF)-α in children infected with Entamoeba histolyticaHuda Muneer AhmedFatima Shihab Al-Nasiri Entamoeba histolytica is an enteric protozoan parasite which caused tissue invasive amoebiasis. It can induce the production of cytokines (such as IL-8 and TNF-α) and chemokines (such as MCP-1) from intestinal epithelial cells. This study was done to detect the serum level of IL-8, MCP-1 and TNF-α, using an enzyme-linked immunosorbent assay, in 60 children affected by amoebiasis to correlate the production of these cytokines with infection and comparison with the healthy persons (n= 30). Three aged groups (1 month- 2 year, 2-4 year and 4-6 year) were entered in the study for patients and healthy controls. The level of IL-8 (37.22 ± 7.06, 37.35 ± 1.981 and 36.45 ± 4.39 pg/ml) in children with amoebiasis were higher significantly compared with the level in healthy children (29.95 ± 4.30, 26.90 ± 4.17 and 25.32 ± 2.31 pg/ml) in all age groups (1 month- 2 year, 2-4 year and 4-6 year, respectively). Significant differences in the level of MCP-1 which were higher (28.92 ± 1.48, 30.85 ± 3.78 and 31.91 ± 6.82 pg/ml) in children with amoebiasis than in healthy children (18.44 ± 0.74, 22.62 ± 6.25 and 18.30 ± 1.43 pg/ml) in all age groups (respectively). Also, the level of TNF-α were higher significantly (58.05 ± 6.90, 55.87 ± 3.81 and 57.32 ± 8.86 pg/ml) in children with amoebiasis compared with healthy children (42.91 ± 2.54, 43.54 ± 3.42 and 42.64 ± 3.47 pg/ml, respectively) in all age groups (respectively). These findings reflect a role of IL-8, MCP-1 and TNF-α in E. histolytica infection. https://tjpsj.org/index.php/tjps/article/view/393Entamoeba histolyticaImmune responseCytokinesChemokines |
| spellingShingle | Huda Muneer Ahmed Fatima Shihab Al-Nasiri Serum level of Interleukin (IL)-8, Monocyte Chemoattractant Protein (MCP)-1 and Tumor Necrosis Factor (TNF)-α in children infected with Entamoeba histolytica Tikrit Journal of Pure Science Entamoeba histolytica Immune response Cytokines Chemokines |
| title | Serum level of Interleukin (IL)-8, Monocyte Chemoattractant Protein (MCP)-1 and Tumor Necrosis Factor (TNF)-α in children infected with Entamoeba histolytica |
| title_full | Serum level of Interleukin (IL)-8, Monocyte Chemoattractant Protein (MCP)-1 and Tumor Necrosis Factor (TNF)-α in children infected with Entamoeba histolytica |
| title_fullStr | Serum level of Interleukin (IL)-8, Monocyte Chemoattractant Protein (MCP)-1 and Tumor Necrosis Factor (TNF)-α in children infected with Entamoeba histolytica |
| title_full_unstemmed | Serum level of Interleukin (IL)-8, Monocyte Chemoattractant Protein (MCP)-1 and Tumor Necrosis Factor (TNF)-α in children infected with Entamoeba histolytica |
| title_short | Serum level of Interleukin (IL)-8, Monocyte Chemoattractant Protein (MCP)-1 and Tumor Necrosis Factor (TNF)-α in children infected with Entamoeba histolytica |
| title_sort | serum level of interleukin il 8 monocyte chemoattractant protein mcp 1 and tumor necrosis factor tnf α in children infected with entamoeba histolytica |
| topic | Entamoeba histolytica Immune response Cytokines Chemokines |
| url | https://tjpsj.org/index.php/tjps/article/view/393 |
| work_keys_str_mv | AT hudamuneerahmed serumlevelofinterleukinil8monocytechemoattractantproteinmcp1andtumornecrosisfactortnfainchildreninfectedwithentamoebahistolytica AT fatimashihabalnasiri serumlevelofinterleukinil8monocytechemoattractantproteinmcp1andtumornecrosisfactortnfainchildreninfectedwithentamoebahistolytica |