First Report of CTX-M-14 Producing Clinical Isolates of Salmonella serovar Typhimurium from Egypt.

Three pediatric isolates of Salmonella serovar Typhimurium from Egypt were reported as CTX-M-14 ESBL producers. Two of the 3 isolates (68 and 111) were 85% related by PFGE;however both were distinct from isolate 94. All three isolates produced multiple clavulanate sensitive β-lactamase bands on IEF...

Full description

Saved in:
Bibliographic Details
Main Authors: Sameh M.M. AbdelGhani, Ellen Smith Moland, Jennifer A. Black, Nancy D. Hanson, Richard V. Goering, Magdy A. Amine, Amal E. Saafan, Maha Gaafar, Mariam Younan, Kenneth S. Thomson
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2009-12-01
Series:Journal of Infection in Developing Countries
Subjects:
Online Access:https://jidc.org/index.php/journal/article/view/386
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849323577427361792
author Sameh M.M. AbdelGhani
Ellen Smith Moland
Jennifer A. Black
Nancy D. Hanson
Richard V. Goering
Magdy A. Amine
Amal E. Saafan
Maha Gaafar
Mariam Younan
Kenneth S. Thomson
author_facet Sameh M.M. AbdelGhani
Ellen Smith Moland
Jennifer A. Black
Nancy D. Hanson
Richard V. Goering
Magdy A. Amine
Amal E. Saafan
Maha Gaafar
Mariam Younan
Kenneth S. Thomson
author_sort Sameh M.M. AbdelGhani
collection DOAJ
description Three pediatric isolates of Salmonella serovar Typhimurium from Egypt were reported as CTX-M-14 ESBL producers. Two of the 3 isolates (68 and 111) were 85% related by PFGE;however both were distinct from isolate 94. All three isolates produced multiple clavulanate sensitive β-lactamase bands on IEF with pIs of 7.9 and 5.4 (all isolates), while pI 8.2 enzyme was produced by isolate 94 and pI 7.4 enzyme by isolate 111. The pI 7.9 enzyme was transferable by conjugation and conferred a CTX-M ESBL phenotype. PCR and sequencing identified the CTX-M gene as CTX-M-14. Genes for OXA-1 and SHV-12 were also identified by sequencing. To our knowledge this is the first report of CTX-M-producing Salmonella in Egypt.
format Article
id doaj-art-bb7738be53a548a58658a402d2c4d777
institution Kabale University
issn 1972-2680
language English
publishDate 2009-12-01
publisher The Journal of Infection in Developing Countries
record_format Article
series Journal of Infection in Developing Countries
spelling doaj-art-bb7738be53a548a58658a402d2c4d7772025-08-20T03:48:58ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802009-12-0140110.3855/jidc.386First Report of CTX-M-14 Producing Clinical Isolates of Salmonella serovar Typhimurium from Egypt.Sameh M.M. AbdelGhani0Ellen Smith Moland1Jennifer A. Black2Nancy D. Hanson3Richard V. Goering4Magdy A. Amine5Amal E. Saafan6Maha Gaafar7Mariam Younan8Kenneth S. Thomson9Cairo University School of PharmacyCreighton University School of MedicineCreighton University School of MedicineCreighton University School of MedicineCreighton University School of MedicineCairo University School of PharmacyBeni-Seif University School of PharmacyCairo University School of MedicineCairo University School of MedicineCreighton University School of MedicineThree pediatric isolates of Salmonella serovar Typhimurium from Egypt were reported as CTX-M-14 ESBL producers. Two of the 3 isolates (68 and 111) were 85% related by PFGE;however both were distinct from isolate 94. All three isolates produced multiple clavulanate sensitive β-lactamase bands on IEF with pIs of 7.9 and 5.4 (all isolates), while pI 8.2 enzyme was produced by isolate 94 and pI 7.4 enzyme by isolate 111. The pI 7.9 enzyme was transferable by conjugation and conferred a CTX-M ESBL phenotype. PCR and sequencing identified the CTX-M gene as CTX-M-14. Genes for OXA-1 and SHV-12 were also identified by sequencing. To our knowledge this is the first report of CTX-M-producing Salmonella in Egypt. https://jidc.org/index.php/journal/article/view/386CTX-M ESBLsEnterobacteriaceaeEgypt
spellingShingle Sameh M.M. AbdelGhani
Ellen Smith Moland
Jennifer A. Black
Nancy D. Hanson
Richard V. Goering
Magdy A. Amine
Amal E. Saafan
Maha Gaafar
Mariam Younan
Kenneth S. Thomson
First Report of CTX-M-14 Producing Clinical Isolates of Salmonella serovar Typhimurium from Egypt.
Journal of Infection in Developing Countries
CTX-M ESBLs
Enterobacteriaceae
Egypt
title First Report of CTX-M-14 Producing Clinical Isolates of Salmonella serovar Typhimurium from Egypt.
title_full First Report of CTX-M-14 Producing Clinical Isolates of Salmonella serovar Typhimurium from Egypt.
title_fullStr First Report of CTX-M-14 Producing Clinical Isolates of Salmonella serovar Typhimurium from Egypt.
title_full_unstemmed First Report of CTX-M-14 Producing Clinical Isolates of Salmonella serovar Typhimurium from Egypt.
title_short First Report of CTX-M-14 Producing Clinical Isolates of Salmonella serovar Typhimurium from Egypt.
title_sort first report of ctx m 14 producing clinical isolates of salmonella serovar typhimurium from egypt
topic CTX-M ESBLs
Enterobacteriaceae
Egypt
url https://jidc.org/index.php/journal/article/view/386
work_keys_str_mv AT samehmmabdelghani firstreportofctxm14producingclinicalisolatesofsalmonellaserovartyphimuriumfromegypt
AT ellensmithmoland firstreportofctxm14producingclinicalisolatesofsalmonellaserovartyphimuriumfromegypt
AT jenniferablack firstreportofctxm14producingclinicalisolatesofsalmonellaserovartyphimuriumfromegypt
AT nancydhanson firstreportofctxm14producingclinicalisolatesofsalmonellaserovartyphimuriumfromegypt
AT richardvgoering firstreportofctxm14producingclinicalisolatesofsalmonellaserovartyphimuriumfromegypt
AT magdyaamine firstreportofctxm14producingclinicalisolatesofsalmonellaserovartyphimuriumfromegypt
AT amalesaafan firstreportofctxm14producingclinicalisolatesofsalmonellaserovartyphimuriumfromegypt
AT mahagaafar firstreportofctxm14producingclinicalisolatesofsalmonellaserovartyphimuriumfromegypt
AT mariamyounan firstreportofctxm14producingclinicalisolatesofsalmonellaserovartyphimuriumfromegypt
AT kennethsthomson firstreportofctxm14producingclinicalisolatesofsalmonellaserovartyphimuriumfromegypt