Modulation of biological activities in adipose derived stem cells by histone deacetylation
Abstract Difficult-to-heal wounds management accounts for about 4% of healthcare costs, highlighting the need for innovative solutions. Extracellular signals drive cell proliferation during tissue regeneration, while epigenetic mechanisms regulate stem cell homeostasis, differentiation, and skin rep...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-84652-1 |
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| author | Sallam Abdallah Mouna Tabebi Sawsan Qanadilo Neserin Ali Jing Wang Pádraig D’Arcy Wen Zhong Folke Sjoberg Moustafa Elmasry Ahmed El-Serafi |
| author_facet | Sallam Abdallah Mouna Tabebi Sawsan Qanadilo Neserin Ali Jing Wang Pádraig D’Arcy Wen Zhong Folke Sjoberg Moustafa Elmasry Ahmed El-Serafi |
| author_sort | Sallam Abdallah |
| collection | DOAJ |
| description | Abstract Difficult-to-heal wounds management accounts for about 4% of healthcare costs, highlighting the need for innovative solutions. Extracellular signals drive cell proliferation during tissue regeneration, while epigenetic mechanisms regulate stem cell homeostasis, differentiation, and skin repair. Exploring epigenetic regulation in adipose-derived stem cells (ADSCs) holds promise for improving skin injury treatments. We investigated the effects of histone deacetylase inhibitor (SAHA) on ADSCs to better understand its cellular and molecular impacts. ADSCs were treated with SAHA for 72 h, showing no change in cell viability at the studied concentrations. However, the expression of histone deacetylase decreased at 1000 nM, while the cell proliferation marker Ki-67 increased after SAHA treatment, as confirmed by immunofluorescence. CCND1 gene expression increased, whereas protein expression of the proliferating cell nuclear antigen (PCNA) decreased. Cell cycle analysis showed an increase in G2 phase in SAHA-treated cells. Microarray analysis revealed 74 upregulated and 40 downregulated differentially expressed genes, including upregulation of P53 targets, CDKN1A and MDM2. Proteomic analysis identified 631 upregulated and 823 downregulated proteins compared to the vehicle. Pathway enrichment analysis showed cell cycle, ATP-dependent chromatin remodeling and DNA processes were among the affected pathways. This study suggests SAHA modulates ADSCs’ biological processes, highlighting its potential for skin regeneration. |
| format | Article |
| id | doaj-art-bb703cbf8e1f4da791dcf838ed201f2e |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-bb703cbf8e1f4da791dcf838ed201f2e2025-02-02T12:16:45ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-024-84652-1Modulation of biological activities in adipose derived stem cells by histone deacetylationSallam Abdallah0Mouna Tabebi1Sawsan Qanadilo2Neserin Ali3Jing Wang4Pádraig D’Arcy5Wen Zhong6Folke Sjoberg7Moustafa Elmasry8Ahmed El-Serafi9The Department of Biomedical and Clinical Sciences (BKV), Linköping UniversityThe Department of Biomedical and Clinical Sciences (BKV), Linköping UniversityDepartment of Biological Sciences, The University of JordanDepartment of Clinical Sciences, Lund UniversityThe Department of Biomedical and Clinical Sciences (BKV), Linköping UniversityThe Department of Biomedical and Clinical Sciences (BKV), Linköping UniversityThe Department of Biomedical and Clinical Sciences (BKV), Linköping UniversityThe Department of Biomedical and Clinical Sciences (BKV), Linköping UniversityThe Department of Biomedical and Clinical Sciences (BKV), Linköping UniversityThe Department of Biomedical and Clinical Sciences (BKV), Linköping UniversityAbstract Difficult-to-heal wounds management accounts for about 4% of healthcare costs, highlighting the need for innovative solutions. Extracellular signals drive cell proliferation during tissue regeneration, while epigenetic mechanisms regulate stem cell homeostasis, differentiation, and skin repair. Exploring epigenetic regulation in adipose-derived stem cells (ADSCs) holds promise for improving skin injury treatments. We investigated the effects of histone deacetylase inhibitor (SAHA) on ADSCs to better understand its cellular and molecular impacts. ADSCs were treated with SAHA for 72 h, showing no change in cell viability at the studied concentrations. However, the expression of histone deacetylase decreased at 1000 nM, while the cell proliferation marker Ki-67 increased after SAHA treatment, as confirmed by immunofluorescence. CCND1 gene expression increased, whereas protein expression of the proliferating cell nuclear antigen (PCNA) decreased. Cell cycle analysis showed an increase in G2 phase in SAHA-treated cells. Microarray analysis revealed 74 upregulated and 40 downregulated differentially expressed genes, including upregulation of P53 targets, CDKN1A and MDM2. Proteomic analysis identified 631 upregulated and 823 downregulated proteins compared to the vehicle. Pathway enrichment analysis showed cell cycle, ATP-dependent chromatin remodeling and DNA processes were among the affected pathways. This study suggests SAHA modulates ADSCs’ biological processes, highlighting its potential for skin regeneration.https://doi.org/10.1038/s41598-024-84652-1Histone deacetylase inhibitorSuberoylanilide hydroxamic acidAdipose derived stem cellsDifferentiationVorinostatEpigenetic |
| spellingShingle | Sallam Abdallah Mouna Tabebi Sawsan Qanadilo Neserin Ali Jing Wang Pádraig D’Arcy Wen Zhong Folke Sjoberg Moustafa Elmasry Ahmed El-Serafi Modulation of biological activities in adipose derived stem cells by histone deacetylation Scientific Reports Histone deacetylase inhibitor Suberoylanilide hydroxamic acid Adipose derived stem cells Differentiation Vorinostat Epigenetic |
| title | Modulation of biological activities in adipose derived stem cells by histone deacetylation |
| title_full | Modulation of biological activities in adipose derived stem cells by histone deacetylation |
| title_fullStr | Modulation of biological activities in adipose derived stem cells by histone deacetylation |
| title_full_unstemmed | Modulation of biological activities in adipose derived stem cells by histone deacetylation |
| title_short | Modulation of biological activities in adipose derived stem cells by histone deacetylation |
| title_sort | modulation of biological activities in adipose derived stem cells by histone deacetylation |
| topic | Histone deacetylase inhibitor Suberoylanilide hydroxamic acid Adipose derived stem cells Differentiation Vorinostat Epigenetic |
| url | https://doi.org/10.1038/s41598-024-84652-1 |
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