Ultrasound-assisted extraction, purification, sulfation of Perilla leaves polysaccharide and hypoglycemic and hypolipidemic activities
Perilla leaves are a common traditional medicinal and edible crop, in order to further explore its medicinal value, after determining the optimal ultrasound-assisted extraction conditions a neutral polysaccharide was extracted. Its structure was analyzed, and sulfation modification was carried out....
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Ultrasonics Sonochemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417725000483 |
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| author | Jing Guan Peng Guo Meng Chen Wenzhao Wang Xinyu Chen Qiuyun Li Yiyang He Jiayue Liang Ningxin Yu Pengna Gao Yanli Wu |
| author_facet | Jing Guan Peng Guo Meng Chen Wenzhao Wang Xinyu Chen Qiuyun Li Yiyang He Jiayue Liang Ningxin Yu Pengna Gao Yanli Wu |
| author_sort | Jing Guan |
| collection | DOAJ |
| description | Perilla leaves are a common traditional medicinal and edible crop, in order to further explore its medicinal value, after determining the optimal ultrasound-assisted extraction conditions a neutral polysaccharide was extracted. Its structure was analyzed, and sulfation modification was carried out. After purification by different column chromatography, the optimal process for sulfation modification of PLP-2-1 was established, and sulfated modified PLP-2-1 (S-PLP-2-1) was successfully prepared. The potential structural framework and molecular weight of PLP-2-1 were determined by high performance liquid chromatography (HPLC), methylation analysis, and nuclear magnetic resonance (NMR) techniques, while sulfated modified S-PLP-2-1 maintained a high degree of structural similarity to PLP-2-1. Particle size analysis, Zeta potential measurement, infrared spectroscopy, and NMR analysis confirmed the presence of sulfate groups in S-PLP-2-1, while thermogravimetric and rheological analyses provided support for the development and utilization of these two polysaccharides. After determined the safe concentration range in vitro, the antioxidant and hypoglycemic activities of PLP-2-1 and S-PLP-2-1 were measured. Besides, the effects on the levels of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) in oleic acid (OA) induced high-fat HepG2 cells of both polysaccharides were determined. The results indicated that the various biological activities of S-PLP-2-1 were significantly enhanced after sulfation modification. Therefore, PLP-2-1 and S-PLP-2-1 have the potential to be developed as drugs for anti-glycolipid metabolism disorders. |
| format | Article |
| id | doaj-art-bb6fcfa59d8849e7939d2c81ecce36de |
| institution | OA Journals |
| issn | 1350-4177 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-bb6fcfa59d8849e7939d2c81ecce36de2025-08-20T02:18:46ZengElsevierUltrasonics Sonochemistry1350-41772025-06-0111710726910.1016/j.ultsonch.2025.107269Ultrasound-assisted extraction, purification, sulfation of Perilla leaves polysaccharide and hypoglycemic and hypolipidemic activitiesJing Guan0Peng Guo1Meng Chen2Wenzhao Wang3Xinyu Chen4Qiuyun Li5Yiyang He6Jiayue Liang7Ningxin Yu8Pengna Gao9Yanli Wu10Department of Organic Chemistry, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang 150081, ChinaDepartment of Organic Chemistry, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang 150081, ChinaDepartment of Organic Chemistry, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang 150081, ChinaDepartment of Organic Chemistry, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang 150081, ChinaDepartment of Organic Chemistry, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang 150081, ChinaDepartment of Organic Chemistry, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang 150081, ChinaDepartment of Organic Chemistry, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang 150081, ChinaDepartment of Organic Chemistry, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang 150081, ChinaDepartment of Organic Chemistry, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang 150081, ChinaDepartment of Organic Chemistry, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang 150081, ChinaCorresponding author at: Department of Organic Chemistry, College of Pharmacy, Harbin Medical University, Harbin 150081, PR China.; Department of Organic Chemistry, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang 150081, ChinaPerilla leaves are a common traditional medicinal and edible crop, in order to further explore its medicinal value, after determining the optimal ultrasound-assisted extraction conditions a neutral polysaccharide was extracted. Its structure was analyzed, and sulfation modification was carried out. After purification by different column chromatography, the optimal process for sulfation modification of PLP-2-1 was established, and sulfated modified PLP-2-1 (S-PLP-2-1) was successfully prepared. The potential structural framework and molecular weight of PLP-2-1 were determined by high performance liquid chromatography (HPLC), methylation analysis, and nuclear magnetic resonance (NMR) techniques, while sulfated modified S-PLP-2-1 maintained a high degree of structural similarity to PLP-2-1. Particle size analysis, Zeta potential measurement, infrared spectroscopy, and NMR analysis confirmed the presence of sulfate groups in S-PLP-2-1, while thermogravimetric and rheological analyses provided support for the development and utilization of these two polysaccharides. After determined the safe concentration range in vitro, the antioxidant and hypoglycemic activities of PLP-2-1 and S-PLP-2-1 were measured. Besides, the effects on the levels of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) in oleic acid (OA) induced high-fat HepG2 cells of both polysaccharides were determined. The results indicated that the various biological activities of S-PLP-2-1 were significantly enhanced after sulfation modification. Therefore, PLP-2-1 and S-PLP-2-1 have the potential to be developed as drugs for anti-glycolipid metabolism disorders.http://www.sciencedirect.com/science/article/pii/S1350417725000483Perilla leaves polysaccharidePurificationSulfation modificationHypoglycemic activityHypolipidemic activity |
| spellingShingle | Jing Guan Peng Guo Meng Chen Wenzhao Wang Xinyu Chen Qiuyun Li Yiyang He Jiayue Liang Ningxin Yu Pengna Gao Yanli Wu Ultrasound-assisted extraction, purification, sulfation of Perilla leaves polysaccharide and hypoglycemic and hypolipidemic activities Ultrasonics Sonochemistry Perilla leaves polysaccharide Purification Sulfation modification Hypoglycemic activity Hypolipidemic activity |
| title | Ultrasound-assisted extraction, purification, sulfation of Perilla leaves polysaccharide and hypoglycemic and hypolipidemic activities |
| title_full | Ultrasound-assisted extraction, purification, sulfation of Perilla leaves polysaccharide and hypoglycemic and hypolipidemic activities |
| title_fullStr | Ultrasound-assisted extraction, purification, sulfation of Perilla leaves polysaccharide and hypoglycemic and hypolipidemic activities |
| title_full_unstemmed | Ultrasound-assisted extraction, purification, sulfation of Perilla leaves polysaccharide and hypoglycemic and hypolipidemic activities |
| title_short | Ultrasound-assisted extraction, purification, sulfation of Perilla leaves polysaccharide and hypoglycemic and hypolipidemic activities |
| title_sort | ultrasound assisted extraction purification sulfation of perilla leaves polysaccharide and hypoglycemic and hypolipidemic activities |
| topic | Perilla leaves polysaccharide Purification Sulfation modification Hypoglycemic activity Hypolipidemic activity |
| url | http://www.sciencedirect.com/science/article/pii/S1350417725000483 |
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