Peculiarities of the genetically modified organisms’ detection in seed batches of rape (Brassica napus L.)
This paper presents the results of molecular studies on testing and evaluation of multiplex test systems for the genetically modified organisms’ (GMOs) identification by PCR in real time. For the detection of genetic modifications in plants of the cabbage family (Brassicaceae) screening of major reg...
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| Format: | Article |
| Language: | English |
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Ukrainian Institute for Plant Variety Examination
2014-03-01
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| Series: | Plant Varieties Studying and Protection |
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| Online Access: | http://journal.sops.gov.ua/article/view/56608 |
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| author | Л. В. Король С. О. Гончарова О. В. Піскова А. В. Костенко І. І. Коровко Л. М. Кожемякіна |
| author_facet | Л. В. Король С. О. Гончарова О. В. Піскова А. В. Костенко І. І. Коровко Л. М. Кожемякіна |
| author_sort | Л. В. Король |
| collection | DOAJ |
| description | This paper presents the results of molecular studies on testing and evaluation of multiplex test systems for the genetically modified organisms’ (GMOs) identification by PCR in real time. For the detection of genetic modifications in plants of the cabbage family (Brassicaceae) screening of major regulatory sequences is insufficient, as in genetic engineering manipulations 35S promoter – of DNA-containing cauliflower mosaic virus (CaMV) is used, which presence in the test material can lead to false positive results. In this work the feasibility of testing for the presence of viral CaMV’s DNA and analysis of screened gene sequences of interest for the usage during the analysis of rapeseed (Brassica napus L.) samples. It was established that the analysis of the presence/absence of a positive result for the CaMV 35S promoter is not giving complete answers to the absence of GMO in the sample. The appropriateness of GMO’s screening in rape seeds with NOS terminator and genes of interest. |
| format | Article |
| id | doaj-art-bb6b380a868c4e4db8b6a3fa1956e661 |
| institution | DOAJ |
| issn | 2518-1017 2518-7457 |
| language | English |
| publishDate | 2014-03-01 |
| publisher | Ukrainian Institute for Plant Variety Examination |
| record_format | Article |
| series | Plant Varieties Studying and Protection |
| spelling | doaj-art-bb6b380a868c4e4db8b6a3fa1956e6612025-08-20T03:10:34ZengUkrainian Institute for Plant Variety ExaminationPlant Varieties Studying and Protection2518-10172518-74572014-03-0101(22)545710.21498/2518-1017.1(22).2014.5660856608Peculiarities of the genetically modified organisms’ detection in seed batches of rape (Brassica napus L.)Л. В. Король0С. О. Гончарова1О. В. Піскова2А. В. Костенко3І. І. Коровко4Л. М. Кожемякіна5Ukrainian Institute for Plant Variety ExaminationUkrainian Institute for Plant Variety ExaminationUkrainian Institute for Plant Variety ExaminationUkrainian Institute for Plant Variety ExaminationUkrainian Institute for Plant Variety ExaminationUkrainian Institute for Plant Variety ExaminationThis paper presents the results of molecular studies on testing and evaluation of multiplex test systems for the genetically modified organisms’ (GMOs) identification by PCR in real time. For the detection of genetic modifications in plants of the cabbage family (Brassicaceae) screening of major regulatory sequences is insufficient, as in genetic engineering manipulations 35S promoter – of DNA-containing cauliflower mosaic virus (CaMV) is used, which presence in the test material can lead to false positive results. In this work the feasibility of testing for the presence of viral CaMV’s DNA and analysis of screened gene sequences of interest for the usage during the analysis of rapeseed (Brassica napus L.) samples. It was established that the analysis of the presence/absence of a positive result for the CaMV 35S promoter is not giving complete answers to the absence of GMO in the sample. The appropriateness of GMO’s screening in rape seeds with NOS terminator and genes of interest.http://journal.sops.gov.ua/article/view/56608rapeGMOscreening sequencesReal-Time PCRcauliflower mosaic virus (CaMV) |
| spellingShingle | Л. В. Король С. О. Гончарова О. В. Піскова А. В. Костенко І. І. Коровко Л. М. Кожемякіна Peculiarities of the genetically modified organisms’ detection in seed batches of rape (Brassica napus L.) Plant Varieties Studying and Protection rape GMO screening sequences Real-Time PCR cauliflower mosaic virus (CaMV) |
| title | Peculiarities of the genetically modified organisms’ detection in seed batches of rape (Brassica napus L.) |
| title_full | Peculiarities of the genetically modified organisms’ detection in seed batches of rape (Brassica napus L.) |
| title_fullStr | Peculiarities of the genetically modified organisms’ detection in seed batches of rape (Brassica napus L.) |
| title_full_unstemmed | Peculiarities of the genetically modified organisms’ detection in seed batches of rape (Brassica napus L.) |
| title_short | Peculiarities of the genetically modified organisms’ detection in seed batches of rape (Brassica napus L.) |
| title_sort | peculiarities of the genetically modified organisms detection in seed batches of rape brassica napus l |
| topic | rape GMO screening sequences Real-Time PCR cauliflower mosaic virus (CaMV) |
| url | http://journal.sops.gov.ua/article/view/56608 |
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