Three New Depsipeptides, Homiamides A–C, Isolated from <i>Streptomyces</i> sp., ROA-065

Three new depsipeptides, homiamides A–C (<b>1</b>–<b>3</b>), were isolated from a marine sediment-derived strain of <i>Streptomyces</i> sp., ROA-065. The planar structures of homiamides A–C (<b>1</b>–<b>3</b>) were elucidated using mass spe...

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Main Authors: Jeong-Hyeon Kim, Ji Young Lee, Juri Lee, Prima F. Hillman, Jihye Lee, Byeongchan Choi, Man-Jeong Paik, Songyi Lee, Sang-Jip Nam
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/29/23/5539
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author Jeong-Hyeon Kim
Ji Young Lee
Juri Lee
Prima F. Hillman
Jihye Lee
Byeongchan Choi
Man-Jeong Paik
Songyi Lee
Sang-Jip Nam
author_facet Jeong-Hyeon Kim
Ji Young Lee
Juri Lee
Prima F. Hillman
Jihye Lee
Byeongchan Choi
Man-Jeong Paik
Songyi Lee
Sang-Jip Nam
author_sort Jeong-Hyeon Kim
collection DOAJ
description Three new depsipeptides, homiamides A–C (<b>1</b>–<b>3</b>), were isolated from a marine sediment-derived strain of <i>Streptomyces</i> sp., ROA-065. The planar structures of homiamides A–C (<b>1</b>–<b>3</b>) were elucidated using mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopic data. The absolute configurations of <b>1</b>–<b>3</b> were deduced from the application of the Marfey’s method and GC-MS analysis after formation of the <i>O</i>-trifluoroacetylated (<i>S</i>)-(+)-methyl-2-butyl ester derivatives of amino acids. Compounds <b>1</b>–<b>3</b> exhibited weak anti-bacterial activities against both Gram-positive bacteria and Gram-negative bacteria, with compound <b>1</b> showing MIC values of 32–64 μg/mL. In antifouling assays, compounds <b>1</b> and <b>2</b> displayed moderate activity against <i>Micrococcus luteus</i> KCTC 3063, while compound <b>3</b> exhibited weak activity against all tested bacteria.
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spelling doaj-art-bb681cc254eb40e4bb76381ca4df6f972024-12-13T16:28:12ZengMDPI AGMolecules1420-30492024-11-012923553910.3390/molecules29235539Three New Depsipeptides, Homiamides A–C, Isolated from <i>Streptomyces</i> sp., ROA-065Jeong-Hyeon Kim0Ji Young Lee1Juri Lee2Prima F. Hillman3Jihye Lee4Byeongchan Choi5Man-Jeong Paik6Songyi Lee7Sang-Jip Nam8Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Republic of KoreaInstitute of Sustainable Earth and Environmental Dynamics (SEED), Pukyong National University, 365 Sinseon-ro, Nam-gu, Busan 48547, Republic of KoreaDepartment of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Republic of KoreaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Kampus Limau Manis, Padang 25163, IndonesiaLaboratories of Marine New Drugs, Redone Technologies Co., Ltd., Jangseong-gun 57247, Republic of KoreaCollege of Pharmacy, Sunchon National University, Suncheon 57922, Republic of KoreaCollege of Pharmacy, Sunchon National University, Suncheon 57922, Republic of KoreaDepartment of Chemistry, Pukyong National University, Busan 48513, Republic of KoreaDepartment of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Republic of KoreaThree new depsipeptides, homiamides A–C (<b>1</b>–<b>3</b>), were isolated from a marine sediment-derived strain of <i>Streptomyces</i> sp., ROA-065. The planar structures of homiamides A–C (<b>1</b>–<b>3</b>) were elucidated using mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopic data. The absolute configurations of <b>1</b>–<b>3</b> were deduced from the application of the Marfey’s method and GC-MS analysis after formation of the <i>O</i>-trifluoroacetylated (<i>S</i>)-(+)-methyl-2-butyl ester derivatives of amino acids. Compounds <b>1</b>–<b>3</b> exhibited weak anti-bacterial activities against both Gram-positive bacteria and Gram-negative bacteria, with compound <b>1</b> showing MIC values of 32–64 μg/mL. In antifouling assays, compounds <b>1</b> and <b>2</b> displayed moderate activity against <i>Micrococcus luteus</i> KCTC 3063, while compound <b>3</b> exhibited weak activity against all tested bacteria.https://www.mdpi.com/1420-3049/29/23/5539<i>Streptomyces</i>depsipeptideantibacterial activityMarfey’s methodgas chromatography mass spectrometry
spellingShingle Jeong-Hyeon Kim
Ji Young Lee
Juri Lee
Prima F. Hillman
Jihye Lee
Byeongchan Choi
Man-Jeong Paik
Songyi Lee
Sang-Jip Nam
Three New Depsipeptides, Homiamides A–C, Isolated from <i>Streptomyces</i> sp., ROA-065
Molecules
<i>Streptomyces</i>
depsipeptide
antibacterial activity
Marfey’s method
gas chromatography mass spectrometry
title Three New Depsipeptides, Homiamides A–C, Isolated from <i>Streptomyces</i> sp., ROA-065
title_full Three New Depsipeptides, Homiamides A–C, Isolated from <i>Streptomyces</i> sp., ROA-065
title_fullStr Three New Depsipeptides, Homiamides A–C, Isolated from <i>Streptomyces</i> sp., ROA-065
title_full_unstemmed Three New Depsipeptides, Homiamides A–C, Isolated from <i>Streptomyces</i> sp., ROA-065
title_short Three New Depsipeptides, Homiamides A–C, Isolated from <i>Streptomyces</i> sp., ROA-065
title_sort three new depsipeptides homiamides a c isolated from i streptomyces i sp roa 065
topic <i>Streptomyces</i>
depsipeptide
antibacterial activity
Marfey’s method
gas chromatography mass spectrometry
url https://www.mdpi.com/1420-3049/29/23/5539
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