LncRNA NONRATT021972 Was Associated with Neuropathic Pain Scoring in Patients with Type 2 Diabetes
Background. Long noncoding RNAs were involved in the processes of diabetes. Our study was aimed to explore clinical potential of LncRNA NONRATT021972 in diabetic neuropathic pain and investigate detailed mechanisms. Methods. 154 patients with type 2 diabetes were enrolled as experimental group paire...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Behavioural Neurology |
| Online Access: | http://dx.doi.org/10.1155/2017/2941297 |
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| author | Wei Yu Guo-qing Zhao Rang-juan Cao Zhi-hua Zhu Kai Li |
| author_facet | Wei Yu Guo-qing Zhao Rang-juan Cao Zhi-hua Zhu Kai Li |
| author_sort | Wei Yu |
| collection | DOAJ |
| description | Background. Long noncoding RNAs were involved in the processes of diabetes. Our study was aimed to explore clinical potential of LncRNA NONRATT021972 in diabetic neuropathic pain and investigate detailed mechanisms. Methods. 154 patients with type 2 diabetes were enrolled as experimental group paired with control. Patients without diabetes but neuropathy were enrolled to explore exclusive role of LncRNA NONRATT021972 in neuropathy. Real-time PCR and ELISA were performed to examine expression of LncRNA and TNF-α in flood. Neuropathic pain scores were calculated with data from NPQ. Streptozotocin was used for SD adult male rats to establish diabetes for NONRATT021972 siRNA or saline treatment. Neuropathic pain behaviors and expression of TNF-α were assessed. Result. Patients with type 2 diabetes had a significantly higher concentration of LncRNA NONRATT021972 in blood and more severe symptoms of neuropathic pain. LncRNA NONRATT021972 was positively associated with neuropathic pain scores of type 2 diabetes. TNF-α level increased in patients with type 2 diabetes. Animal experiment showed that LncRNA NONRATT021972 siRNA attenuated inflammation via decreasing TNF-α and alleviated neuropathic pain. Conclusion. LncRNA NONRATT021972 increased in type 2 diabetes and was positively associated with neuropathic pain scoring in type 2 diabetes. LncRNA NONRATT021972 exacerbated neuropathic pain via TNF-α related pathways. |
| format | Article |
| id | doaj-art-bb62f8dec820453aa90f2d59013eb668 |
| institution | OA Journals |
| issn | 0953-4180 1875-8584 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Behavioural Neurology |
| spelling | doaj-art-bb62f8dec820453aa90f2d59013eb6682025-08-20T02:18:46ZengWileyBehavioural Neurology0953-41801875-85842017-01-01201710.1155/2017/29412972941297LncRNA NONRATT021972 Was Associated with Neuropathic Pain Scoring in Patients with Type 2 DiabetesWei Yu0Guo-qing Zhao1Rang-juan Cao2Zhi-hua Zhu3Kai Li4Hand-Surgery Department, China-Japan Union Hospital of Jilin University, Changchun, ChinaAnesthesia Department, China-Japan Union Hospital of Jilin University, Changchun, ChinaHand-Surgery Department, China-Japan Union Hospital of Jilin University, Changchun, ChinaAnesthesia Department, China-Japan Union Hospital of Jilin University, Changchun, ChinaAnesthesia Department, China-Japan Union Hospital of Jilin University, Changchun, ChinaBackground. Long noncoding RNAs were involved in the processes of diabetes. Our study was aimed to explore clinical potential of LncRNA NONRATT021972 in diabetic neuropathic pain and investigate detailed mechanisms. Methods. 154 patients with type 2 diabetes were enrolled as experimental group paired with control. Patients without diabetes but neuropathy were enrolled to explore exclusive role of LncRNA NONRATT021972 in neuropathy. Real-time PCR and ELISA were performed to examine expression of LncRNA and TNF-α in flood. Neuropathic pain scores were calculated with data from NPQ. Streptozotocin was used for SD adult male rats to establish diabetes for NONRATT021972 siRNA or saline treatment. Neuropathic pain behaviors and expression of TNF-α were assessed. Result. Patients with type 2 diabetes had a significantly higher concentration of LncRNA NONRATT021972 in blood and more severe symptoms of neuropathic pain. LncRNA NONRATT021972 was positively associated with neuropathic pain scores of type 2 diabetes. TNF-α level increased in patients with type 2 diabetes. Animal experiment showed that LncRNA NONRATT021972 siRNA attenuated inflammation via decreasing TNF-α and alleviated neuropathic pain. Conclusion. LncRNA NONRATT021972 increased in type 2 diabetes and was positively associated with neuropathic pain scoring in type 2 diabetes. LncRNA NONRATT021972 exacerbated neuropathic pain via TNF-α related pathways.http://dx.doi.org/10.1155/2017/2941297 |
| spellingShingle | Wei Yu Guo-qing Zhao Rang-juan Cao Zhi-hua Zhu Kai Li LncRNA NONRATT021972 Was Associated with Neuropathic Pain Scoring in Patients with Type 2 Diabetes Behavioural Neurology |
| title | LncRNA NONRATT021972 Was Associated with Neuropathic Pain Scoring in Patients with Type 2 Diabetes |
| title_full | LncRNA NONRATT021972 Was Associated with Neuropathic Pain Scoring in Patients with Type 2 Diabetes |
| title_fullStr | LncRNA NONRATT021972 Was Associated with Neuropathic Pain Scoring in Patients with Type 2 Diabetes |
| title_full_unstemmed | LncRNA NONRATT021972 Was Associated with Neuropathic Pain Scoring in Patients with Type 2 Diabetes |
| title_short | LncRNA NONRATT021972 Was Associated with Neuropathic Pain Scoring in Patients with Type 2 Diabetes |
| title_sort | lncrna nonratt021972 was associated with neuropathic pain scoring in patients with type 2 diabetes |
| url | http://dx.doi.org/10.1155/2017/2941297 |
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