Analysis of the Expression and Function of Immunoglobulin-Like Transcript 4 (ILT4, LILRB2) in Dendritic Cells from Patients with Systemic Lupus Erythematosus
Dendritic cells (DC) play an important role in the development and maintenance of immune tolerance. Although the inhibitory receptor ILT4/LILRB2 has been related with the tolerogenic phenotype of DC, the possible role of this receptor in the breakdown of DC tolerogenic function in systemic lupus ery...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/4163094 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832562972403171328 |
|---|---|
| author | Paola del Carmen Guerra-de Blas Yael Sebastián Villaseñor-Talavera Daniela de Jesús Cruz-González Lourdes Baranda Lesly Doníz-Padilla Carlos Abud-Mendoza Roberto González-Amaro Adriana Elizabeth Monsiváis-Urenda |
| author_facet | Paola del Carmen Guerra-de Blas Yael Sebastián Villaseñor-Talavera Daniela de Jesús Cruz-González Lourdes Baranda Lesly Doníz-Padilla Carlos Abud-Mendoza Roberto González-Amaro Adriana Elizabeth Monsiváis-Urenda |
| author_sort | Paola del Carmen Guerra-de Blas |
| collection | DOAJ |
| description | Dendritic cells (DC) play an important role in the development and maintenance of immune tolerance. Although the inhibitory receptor ILT4/LILRB2 has been related with the tolerogenic phenotype of DC, the possible role of this receptor in the breakdown of DC tolerogenic function in systemic lupus erythematosus (SLE) has not been elucidated. In this study, we analyzed the expression and function of the inhibitory receptor ILT4 in DC from SLE patients. We found that the percentage of ILT4 positive plasmacytoid DC and myeloid DC is significantly diminished in SLE patients. Interestingly, ligation of ILT4 did not affect the maturation or immunogenic capability of DC in healthy controls. In contrast, in SLE patients we observed an inhibitory effect of ILT4 on the immunogenic capability of DC. ILT4 was shown not to have a crucial role in regulating the maturation and function of DC from healthy controls but is partially involved in the maturation process and immunogenic capability of DC from SLE patients, suggesting that other inhibitory receptors, involved in the regulation of DC tolerogenic function, may be impaired in this autoimmune disease. |
| format | Article |
| id | doaj-art-bb58e26b9fda4a2e914bab2a3aebe01a |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-bb58e26b9fda4a2e914bab2a3aebe01a2025-02-03T01:21:22ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/41630944163094Analysis of the Expression and Function of Immunoglobulin-Like Transcript 4 (ILT4, LILRB2) in Dendritic Cells from Patients with Systemic Lupus ErythematosusPaola del Carmen Guerra-de Blas0Yael Sebastián Villaseñor-Talavera1Daniela de Jesús Cruz-González2Lourdes Baranda3Lesly Doníz-Padilla4Carlos Abud-Mendoza5Roberto González-Amaro6Adriana Elizabeth Monsiváis-Urenda7Departamento de Inmunología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210 San Luis Potosí, SLP, MexicoDepartamento de Inmunología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210 San Luis Potosí, SLP, MexicoDepartamento de Inmunología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210 San Luis Potosí, SLP, MexicoDepartamento de Inmunología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210 San Luis Potosí, SLP, MexicoDepartamento de Inmunología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210 San Luis Potosí, SLP, MexicoDepartamento de Reumatología, Hospital Central “Dr. Ignacio Morones Prieto”, Avenida Venustiano Carranza 2395, 78290 San Luis Potosí, SLP, MexicoDepartamento de Inmunología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210 San Luis Potosí, SLP, MexicoDepartamento de Inmunología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210 San Luis Potosí, SLP, MexicoDendritic cells (DC) play an important role in the development and maintenance of immune tolerance. Although the inhibitory receptor ILT4/LILRB2 has been related with the tolerogenic phenotype of DC, the possible role of this receptor in the breakdown of DC tolerogenic function in systemic lupus erythematosus (SLE) has not been elucidated. In this study, we analyzed the expression and function of the inhibitory receptor ILT4 in DC from SLE patients. We found that the percentage of ILT4 positive plasmacytoid DC and myeloid DC is significantly diminished in SLE patients. Interestingly, ligation of ILT4 did not affect the maturation or immunogenic capability of DC in healthy controls. In contrast, in SLE patients we observed an inhibitory effect of ILT4 on the immunogenic capability of DC. ILT4 was shown not to have a crucial role in regulating the maturation and function of DC from healthy controls but is partially involved in the maturation process and immunogenic capability of DC from SLE patients, suggesting that other inhibitory receptors, involved in the regulation of DC tolerogenic function, may be impaired in this autoimmune disease.http://dx.doi.org/10.1155/2016/4163094 |
| spellingShingle | Paola del Carmen Guerra-de Blas Yael Sebastián Villaseñor-Talavera Daniela de Jesús Cruz-González Lourdes Baranda Lesly Doníz-Padilla Carlos Abud-Mendoza Roberto González-Amaro Adriana Elizabeth Monsiváis-Urenda Analysis of the Expression and Function of Immunoglobulin-Like Transcript 4 (ILT4, LILRB2) in Dendritic Cells from Patients with Systemic Lupus Erythematosus Journal of Immunology Research |
| title | Analysis of the Expression and Function of Immunoglobulin-Like Transcript 4 (ILT4, LILRB2) in Dendritic Cells from Patients with Systemic Lupus Erythematosus |
| title_full | Analysis of the Expression and Function of Immunoglobulin-Like Transcript 4 (ILT4, LILRB2) in Dendritic Cells from Patients with Systemic Lupus Erythematosus |
| title_fullStr | Analysis of the Expression and Function of Immunoglobulin-Like Transcript 4 (ILT4, LILRB2) in Dendritic Cells from Patients with Systemic Lupus Erythematosus |
| title_full_unstemmed | Analysis of the Expression and Function of Immunoglobulin-Like Transcript 4 (ILT4, LILRB2) in Dendritic Cells from Patients with Systemic Lupus Erythematosus |
| title_short | Analysis of the Expression and Function of Immunoglobulin-Like Transcript 4 (ILT4, LILRB2) in Dendritic Cells from Patients with Systemic Lupus Erythematosus |
| title_sort | analysis of the expression and function of immunoglobulin like transcript 4 ilt4 lilrb2 in dendritic cells from patients with systemic lupus erythematosus |
| url | http://dx.doi.org/10.1155/2016/4163094 |
| work_keys_str_mv | AT paoladelcarmenguerradeblas analysisoftheexpressionandfunctionofimmunoglobulinliketranscript4ilt4lilrb2indendriticcellsfrompatientswithsystemiclupuserythematosus AT yaelsebastianvillasenortalavera analysisoftheexpressionandfunctionofimmunoglobulinliketranscript4ilt4lilrb2indendriticcellsfrompatientswithsystemiclupuserythematosus AT danieladejesuscruzgonzalez analysisoftheexpressionandfunctionofimmunoglobulinliketranscript4ilt4lilrb2indendriticcellsfrompatientswithsystemiclupuserythematosus AT lourdesbaranda analysisoftheexpressionandfunctionofimmunoglobulinliketranscript4ilt4lilrb2indendriticcellsfrompatientswithsystemiclupuserythematosus AT leslydonizpadilla analysisoftheexpressionandfunctionofimmunoglobulinliketranscript4ilt4lilrb2indendriticcellsfrompatientswithsystemiclupuserythematosus AT carlosabudmendoza analysisoftheexpressionandfunctionofimmunoglobulinliketranscript4ilt4lilrb2indendriticcellsfrompatientswithsystemiclupuserythematosus AT robertogonzalezamaro analysisoftheexpressionandfunctionofimmunoglobulinliketranscript4ilt4lilrb2indendriticcellsfrompatientswithsystemiclupuserythematosus AT adrianaelizabethmonsivaisurenda analysisoftheexpressionandfunctionofimmunoglobulinliketranscript4ilt4lilrb2indendriticcellsfrompatientswithsystemiclupuserythematosus |