Induction of UV-B Stress Tolerance by Momilactones and Gibberellic Acid in Rice
This study aims to investigate the effects of momilactone-rich bran fractions (MRBF) and gibberellic acid (GA3) on the physiological responses, biochemical composition, and antioxidant activity in rice (<i>Oryza sativa</i> L.) under UV-B stress. Rice seedlings were treated with MRBF (3 m...
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MDPI AG
2025-02-01
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| author | Ramin Rayee La Hoang Anh Tran Dang Xuan |
| author_facet | Ramin Rayee La Hoang Anh Tran Dang Xuan |
| author_sort | Ramin Rayee |
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| description | This study aims to investigate the effects of momilactone-rich bran fractions (MRBF) and gibberellic acid (GA3) on the physiological responses, biochemical composition, and antioxidant activity in rice (<i>Oryza sativa</i> L.) under UV-B stress. Rice seedlings were treated with MRBF (3 mg mL<sup>−1</sup>), or GA3 (50 and 100 µM) by foliar spraying, then exposed to UV-B-induced stress for 0, 1.5, and 3 h. Phenolic acids and endogenous momilactones A (MB) and B (MB) were analyzed using high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography–electrospray ionization–mass spectrometry (UPLC-ESI-MS). The results demonstrated that UV-B stress reduced chlorophyll and carotenoid, which were significantly elevated by GA3 and GA3 + MRBF treatments. MRBF alone increased proline concentrations by 18.33%, while GA3 significantly enhanced total flavonoid content (TFC), and GA3 + MRBF raised total phenolic content (TPC). Among identified phenolic acids, sinapic, ferulic, and salicylic acids were notably elevated under MRBF and 3 h UV-B stress, while cinnamic and gallic acids were markedly reduced. The highest endogenous MA content was found in MRBF-treated plants under 1.5 h UV-B stress, with significant MA and MB concentrations also in plants treated with 50 µM GA3 + MRBF after extended UV-B exposure. This combination also enhanced antioxidant activities and reduced rice seedlings’ oxidative stress index (OSI) by 30.55%. Correlation analysis showed a significant relationship of MA with TPC and proline. Together, these results support the potential role of momilactones in regulating UV-B stress tolerance in rice; however, the molecular mechanisms underlying this regulation require further investigation. |
| format | Article |
| id | doaj-art-bb5605204ba04c1fa6cba06ddb690aff |
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| issn | 2673-7655 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-bb5605204ba04c1fa6cba06ddb690aff2025-08-20T02:44:49ZengMDPI AGCrops2673-76552025-02-0151610.3390/crops5010006Induction of UV-B Stress Tolerance by Momilactones and Gibberellic Acid in RiceRamin Rayee0La Hoang Anh1Tran Dang Xuan2Transdisciplinary Science and Engineering Program, Graduate School of Advanced Science and Engineering, Hiroshima University, Hiroshima 739-8529, JapanTransdisciplinary Science and Engineering Program, Graduate School of Advanced Science and Engineering, Hiroshima University, Hiroshima 739-8529, JapanTransdisciplinary Science and Engineering Program, Graduate School of Advanced Science and Engineering, Hiroshima University, Hiroshima 739-8529, JapanThis study aims to investigate the effects of momilactone-rich bran fractions (MRBF) and gibberellic acid (GA3) on the physiological responses, biochemical composition, and antioxidant activity in rice (<i>Oryza sativa</i> L.) under UV-B stress. Rice seedlings were treated with MRBF (3 mg mL<sup>−1</sup>), or GA3 (50 and 100 µM) by foliar spraying, then exposed to UV-B-induced stress for 0, 1.5, and 3 h. Phenolic acids and endogenous momilactones A (MB) and B (MB) were analyzed using high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography–electrospray ionization–mass spectrometry (UPLC-ESI-MS). The results demonstrated that UV-B stress reduced chlorophyll and carotenoid, which were significantly elevated by GA3 and GA3 + MRBF treatments. MRBF alone increased proline concentrations by 18.33%, while GA3 significantly enhanced total flavonoid content (TFC), and GA3 + MRBF raised total phenolic content (TPC). Among identified phenolic acids, sinapic, ferulic, and salicylic acids were notably elevated under MRBF and 3 h UV-B stress, while cinnamic and gallic acids were markedly reduced. The highest endogenous MA content was found in MRBF-treated plants under 1.5 h UV-B stress, with significant MA and MB concentrations also in plants treated with 50 µM GA3 + MRBF after extended UV-B exposure. This combination also enhanced antioxidant activities and reduced rice seedlings’ oxidative stress index (OSI) by 30.55%. Correlation analysis showed a significant relationship of MA with TPC and proline. Together, these results support the potential role of momilactones in regulating UV-B stress tolerance in rice; however, the molecular mechanisms underlying this regulation require further investigation.https://www.mdpi.com/2673-7655/5/1/6GA3UV-B stressricebioactive compoundsantioxidant activities |
| spellingShingle | Ramin Rayee La Hoang Anh Tran Dang Xuan Induction of UV-B Stress Tolerance by Momilactones and Gibberellic Acid in Rice Crops GA3 UV-B stress rice bioactive compounds antioxidant activities |
| title | Induction of UV-B Stress Tolerance by Momilactones and Gibberellic Acid in Rice |
| title_full | Induction of UV-B Stress Tolerance by Momilactones and Gibberellic Acid in Rice |
| title_fullStr | Induction of UV-B Stress Tolerance by Momilactones and Gibberellic Acid in Rice |
| title_full_unstemmed | Induction of UV-B Stress Tolerance by Momilactones and Gibberellic Acid in Rice |
| title_short | Induction of UV-B Stress Tolerance by Momilactones and Gibberellic Acid in Rice |
| title_sort | induction of uv b stress tolerance by momilactones and gibberellic acid in rice |
| topic | GA3 UV-B stress rice bioactive compounds antioxidant activities |
| url | https://www.mdpi.com/2673-7655/5/1/6 |
| work_keys_str_mv | AT raminrayee inductionofuvbstresstolerancebymomilactonesandgibberellicacidinrice AT lahoanganh inductionofuvbstresstolerancebymomilactonesandgibberellicacidinrice AT trandangxuan inductionofuvbstresstolerancebymomilactonesandgibberellicacidinrice |