Flow cytometry method for determining the kariotype of tea somatic clones isolated in vitro (camellia sinensis (l.) O. Kuntze)
The method of flow cytometry (on Beckman Coulter company cytometer) has been used to study the genetic variation of the karyotype in 15 somatic clones of tea plants isolated in vitro. Somatic clones have been obtained by inducing gemmogenesis from a callus culture of tea micro sprouts, which have be...
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Maykop State Technological University
2019-09-01
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| Series: | Новые технологии |
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| Online Access: | https://newtechology.mkgtu.ru/jour/article/view/324 |
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| author | M. V. Gvasaliya L. S. Samarina |
| author_facet | M. V. Gvasaliya L. S. Samarina |
| author_sort | M. V. Gvasaliya |
| collection | DOAJ |
| description | The method of flow cytometry (on Beckman Coulter company cytometer) has been used to study the genetic variation of the karyotype in 15 somatic clones of tea plants isolated in vitro. Somatic clones have been obtained by inducing gemmogenesis from a callus culture of tea micro sprouts, which have been in vitro root-to-seed for 8 years. The Murashige and Skoog modified mineral base (MS) with an addition of growth regulators of 6 – BAP – 2,5 ml + NAA – 0,2 ml + HA – 1,0 ml + mesoinositol – 100 mg have been the basic nutrient medium for cultivating somatic clones. Changes in the karyotype have been recorded by analysis of the genome size. The Colchis tea genotype of 2n = 30 and the Allium cepa 2n = 18 monitor sample (32,07 pg DNA) have been used as external standards. As a result of the studies, genome size variability has been detected in 3 of the 15 tea somatic clones. In somatic clones (Sc – 11; Sc – 27; Sc – 33) the genome size is 7,26-8,70 pg (picograms) of DNA compared with the control genotype of the diploid Colchis variety, whose genome size is 5.08 pg of DNA. The presence of somaclonal variability in somatic clones isolated by phenotypic traits (Sc – 11; Sc – 27; Sc – 33) has been confirmed at the karyotype level. |
| format | Article |
| id | doaj-art-bb522dbe84e44b6ebd2534b779032218 |
| institution | DOAJ |
| issn | 2072-0920 2713-0029 |
| language | Russian |
| publishDate | 2019-09-01 |
| publisher | Maykop State Technological University |
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| series | Новые технологии |
| spelling | doaj-art-bb522dbe84e44b6ebd2534b7790322182025-08-20T02:49:19ZrusMaykop State Technological UniversityНовые технологии2072-09202713-00292019-09-010315616310.24411/2072-0920-2019-10314322Flow cytometry method for determining the kariotype of tea somatic clones isolated in vitro (camellia sinensis (l.) O. Kuntze)M. V. Gvasaliya0L. S. Samarina1FSBSI “All-Russian Research Institute of Floriculture and Subtropical Crops”FSBSI “All-Russian Research Institute of Floriculture and Subtropical Crops”The method of flow cytometry (on Beckman Coulter company cytometer) has been used to study the genetic variation of the karyotype in 15 somatic clones of tea plants isolated in vitro. Somatic clones have been obtained by inducing gemmogenesis from a callus culture of tea micro sprouts, which have been in vitro root-to-seed for 8 years. The Murashige and Skoog modified mineral base (MS) with an addition of growth regulators of 6 – BAP – 2,5 ml + NAA – 0,2 ml + HA – 1,0 ml + mesoinositol – 100 mg have been the basic nutrient medium for cultivating somatic clones. Changes in the karyotype have been recorded by analysis of the genome size. The Colchis tea genotype of 2n = 30 and the Allium cepa 2n = 18 monitor sample (32,07 pg DNA) have been used as external standards. As a result of the studies, genome size variability has been detected in 3 of the 15 tea somatic clones. In somatic clones (Sc – 11; Sc – 27; Sc – 33) the genome size is 7,26-8,70 pg (picograms) of DNA compared with the control genotype of the diploid Colchis variety, whose genome size is 5.08 pg of DNA. The presence of somaclonal variability in somatic clones isolated by phenotypic traits (Sc – 11; Sc – 27; Sc – 33) has been confirmed at the karyotype level.https://newtechology.mkgtu.ru/jour/article/view/324camellia sinensis (l.) o. kuntzeflow cytometry methodsomaclonal variation in vitrotea somatic cloneskaryotypegenome size |
| spellingShingle | M. V. Gvasaliya L. S. Samarina Flow cytometry method for determining the kariotype of tea somatic clones isolated in vitro (camellia sinensis (l.) O. Kuntze) Новые технологии camellia sinensis (l.) o. kuntze flow cytometry method somaclonal variation in vitro tea somatic clones karyotype genome size |
| title | Flow cytometry method for determining the kariotype of tea somatic clones isolated in vitro (camellia sinensis (l.) O. Kuntze) |
| title_full | Flow cytometry method for determining the kariotype of tea somatic clones isolated in vitro (camellia sinensis (l.) O. Kuntze) |
| title_fullStr | Flow cytometry method for determining the kariotype of tea somatic clones isolated in vitro (camellia sinensis (l.) O. Kuntze) |
| title_full_unstemmed | Flow cytometry method for determining the kariotype of tea somatic clones isolated in vitro (camellia sinensis (l.) O. Kuntze) |
| title_short | Flow cytometry method for determining the kariotype of tea somatic clones isolated in vitro (camellia sinensis (l.) O. Kuntze) |
| title_sort | flow cytometry method for determining the kariotype of tea somatic clones isolated in vitro camellia sinensis l o kuntze |
| topic | camellia sinensis (l.) o. kuntze flow cytometry method somaclonal variation in vitro tea somatic clones karyotype genome size |
| url | https://newtechology.mkgtu.ru/jour/article/view/324 |
| work_keys_str_mv | AT mvgvasaliya flowcytometrymethodfordeterminingthekariotypeofteasomaticclonesisolatedinvitrocamelliasinensislokuntze AT lssamarina flowcytometrymethodfordeterminingthekariotypeofteasomaticclonesisolatedinvitrocamelliasinensislokuntze |