Genetic association between glucocorticoid receptor gene Bcl1 rs41423247 and rs6198 polymorphisms and risk of obesity in Egyptian children

Abstract Obesity represents a major global public-health problem during childhood and adolescence. The genetic contribution to obesity and its consequences is well-established. Variation in glucocorticoid (GC)-sensitivity can be partly explained by polymorphisms in GC receptor (GR) gene where NR3C1;...

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Main Authors: Nanees A. Salem, Angi A. Alwakeel, Mayada Abdel-Latif, Shaimaa R. Hendawy, Mai S. Korkor
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-94447-7
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author Nanees A. Salem
Angi A. Alwakeel
Mayada Abdel-Latif
Shaimaa R. Hendawy
Mai S. Korkor
author_facet Nanees A. Salem
Angi A. Alwakeel
Mayada Abdel-Latif
Shaimaa R. Hendawy
Mai S. Korkor
author_sort Nanees A. Salem
collection DOAJ
description Abstract Obesity represents a major global public-health problem during childhood and adolescence. The genetic contribution to obesity and its consequences is well-established. Variation in glucocorticoid (GC)-sensitivity can be partly explained by polymorphisms in GC receptor (GR) gene where NR3C1; Bcl1 rs41423247 and NR3C1 rs6198 single nucleotide polymorphisms (SNPs) have been linked to higher and lower GC sensitivity, respectively. We aimed to explore the potential association between the GR gene SNPs and risk of obesity in a cohort of Egyptian children. We included 100 pre-pubertal children; 60 obese children and 40 age-and sex-matched normal-weight controls. Bcl1 rs41423247 SNP was genotyped using PCR-restriction fragment length polymorphism technique and NR3C1 rs6198 SNP was genotyped using Real-time-PCR.In Bcl1 rs41423247, obese children had more frequent CG, GG genotypes and G allele compared to healthy controls (P = 0.039, 0.019 and 0.007 respectively). Moreover, insulin resistance was significantly higher in combined CG + GG group compared to CC group. On the contrary, no significant differences were found in genotypes, alleles frequencies or insulin resistance between obese and non-obese children in NR3C1 rs6198. GR Bcl1 rs41423247 gene polymorphism may play a role in genetic susceptibility to obesity that can be a future targeted therapy for obesity.
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spelling doaj-art-bb52259bf3d545f2bacd34573aa4243e2025-08-20T02:11:46ZengNature PortfolioScientific Reports2045-23222025-04-0115111010.1038/s41598-025-94447-7Genetic association between glucocorticoid receptor gene Bcl1 rs41423247 and rs6198 polymorphisms and risk of obesity in Egyptian childrenNanees A. Salem0Angi A. Alwakeel1Mayada Abdel-Latif2Shaimaa R. Hendawy3Mai S. Korkor4Pediatric Department, Faculty of Medicine, Mansoura UniversityPediatric Department, Faculty of Medicine, Mansoura UniversityPediatric Department, Faculty of Medicine, Mansoura UniversityClinical Pathology department, Faculty of Medicine, Mansoura UniversityPediatric Department, Faculty of Medicine, Mansoura UniversityAbstract Obesity represents a major global public-health problem during childhood and adolescence. The genetic contribution to obesity and its consequences is well-established. Variation in glucocorticoid (GC)-sensitivity can be partly explained by polymorphisms in GC receptor (GR) gene where NR3C1; Bcl1 rs41423247 and NR3C1 rs6198 single nucleotide polymorphisms (SNPs) have been linked to higher and lower GC sensitivity, respectively. We aimed to explore the potential association between the GR gene SNPs and risk of obesity in a cohort of Egyptian children. We included 100 pre-pubertal children; 60 obese children and 40 age-and sex-matched normal-weight controls. Bcl1 rs41423247 SNP was genotyped using PCR-restriction fragment length polymorphism technique and NR3C1 rs6198 SNP was genotyped using Real-time-PCR.In Bcl1 rs41423247, obese children had more frequent CG, GG genotypes and G allele compared to healthy controls (P = 0.039, 0.019 and 0.007 respectively). Moreover, insulin resistance was significantly higher in combined CG + GG group compared to CC group. On the contrary, no significant differences were found in genotypes, alleles frequencies or insulin resistance between obese and non-obese children in NR3C1 rs6198. GR Bcl1 rs41423247 gene polymorphism may play a role in genetic susceptibility to obesity that can be a future targeted therapy for obesity.https://doi.org/10.1038/s41598-025-94447-7GlucocorticoidBcl1 gene polymorphismObesityChildren
spellingShingle Nanees A. Salem
Angi A. Alwakeel
Mayada Abdel-Latif
Shaimaa R. Hendawy
Mai S. Korkor
Genetic association between glucocorticoid receptor gene Bcl1 rs41423247 and rs6198 polymorphisms and risk of obesity in Egyptian children
Scientific Reports
Glucocorticoid
Bcl1 gene polymorphism
Obesity
Children
title Genetic association between glucocorticoid receptor gene Bcl1 rs41423247 and rs6198 polymorphisms and risk of obesity in Egyptian children
title_full Genetic association between glucocorticoid receptor gene Bcl1 rs41423247 and rs6198 polymorphisms and risk of obesity in Egyptian children
title_fullStr Genetic association between glucocorticoid receptor gene Bcl1 rs41423247 and rs6198 polymorphisms and risk of obesity in Egyptian children
title_full_unstemmed Genetic association between glucocorticoid receptor gene Bcl1 rs41423247 and rs6198 polymorphisms and risk of obesity in Egyptian children
title_short Genetic association between glucocorticoid receptor gene Bcl1 rs41423247 and rs6198 polymorphisms and risk of obesity in Egyptian children
title_sort genetic association between glucocorticoid receptor gene bcl1 rs41423247 and rs6198 polymorphisms and risk of obesity in egyptian children
topic Glucocorticoid
Bcl1 gene polymorphism
Obesity
Children
url https://doi.org/10.1038/s41598-025-94447-7
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