Application of the Insect Cell-Baculovirus Expression Vector System in Adeno-Associated Viral Production
Insect Cell-Baculovirus Expression Vector System (IC-BEVS) is an efficient protein expression platform, which is famous for its high-level expression of complex protein in insect cells. The system is based on baculoviruses such as <i>Autographa californica</i> multiple nucleopolyhedrovir...
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2024-11-01
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| author | Fei Wang Jiawen Sun Wenyan Guo Yang Wu |
| author_facet | Fei Wang Jiawen Sun Wenyan Guo Yang Wu |
| author_sort | Fei Wang |
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| description | Insect Cell-Baculovirus Expression Vector System (IC-BEVS) is an efficient protein expression platform, which is famous for its high-level expression of complex protein in insect cells. The system is based on baculoviruses such as <i>Autographa californica</i> multiple nucleopolyhedrovirus (AcMNPV), and the expression efficiency of the target proteins has been significantly improved by optimizing the viral vectors and cell lines. In recent years, IC-BEVS have shown great potential for Adeno-Associated Virus (AAV) production, particularly excelling in AAV structural protein expression and recombinant AAV production. The system not only improves the yield and purity of AAV, but also shortens the production cycle, providing an efficient and reliable tool for gene therapy. However, the system also has some challenges, including protein modification differences, limitations in expression levels, and production costs. This paper reviews the development of the insect baculovirus expression system, its application in AAV production, and its prospects in gene therapy, aiming to provide a systematic reference and outlook for research in related fields. |
| format | Article |
| id | doaj-art-bb3fcaf7e73249828e7055c25f39f13e |
| institution | OA Journals |
| issn | 2076-3417 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-bb3fcaf7e73249828e7055c25f39f13e2025-08-20T01:55:34ZengMDPI AGApplied Sciences2076-34172024-11-0114231094810.3390/app142310948Application of the Insect Cell-Baculovirus Expression Vector System in Adeno-Associated Viral ProductionFei Wang0Jiawen Sun1Wenyan Guo2Yang Wu3College of Life Science and Chemistry, Hunan University of Technology, Zhuzhou 412007, ChinaCollege of Life Science and Chemistry, Hunan University of Technology, Zhuzhou 412007, ChinaCollege of Life Science and Chemistry, Hunan University of Technology, Zhuzhou 412007, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Center for Magnetic Resonance, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, ChinaInsect Cell-Baculovirus Expression Vector System (IC-BEVS) is an efficient protein expression platform, which is famous for its high-level expression of complex protein in insect cells. The system is based on baculoviruses such as <i>Autographa californica</i> multiple nucleopolyhedrovirus (AcMNPV), and the expression efficiency of the target proteins has been significantly improved by optimizing the viral vectors and cell lines. In recent years, IC-BEVS have shown great potential for Adeno-Associated Virus (AAV) production, particularly excelling in AAV structural protein expression and recombinant AAV production. The system not only improves the yield and purity of AAV, but also shortens the production cycle, providing an efficient and reliable tool for gene therapy. However, the system also has some challenges, including protein modification differences, limitations in expression levels, and production costs. This paper reviews the development of the insect baculovirus expression system, its application in AAV production, and its prospects in gene therapy, aiming to provide a systematic reference and outlook for research in related fields.https://www.mdpi.com/2076-3417/14/23/10948Baculovirus Expression Vector System (BEVS)insect cellsAdeno-Associated Virus (AAV)gene therapylarge-scale production |
| spellingShingle | Fei Wang Jiawen Sun Wenyan Guo Yang Wu Application of the Insect Cell-Baculovirus Expression Vector System in Adeno-Associated Viral Production Applied Sciences Baculovirus Expression Vector System (BEVS) insect cells Adeno-Associated Virus (AAV) gene therapy large-scale production |
| title | Application of the Insect Cell-Baculovirus Expression Vector System in Adeno-Associated Viral Production |
| title_full | Application of the Insect Cell-Baculovirus Expression Vector System in Adeno-Associated Viral Production |
| title_fullStr | Application of the Insect Cell-Baculovirus Expression Vector System in Adeno-Associated Viral Production |
| title_full_unstemmed | Application of the Insect Cell-Baculovirus Expression Vector System in Adeno-Associated Viral Production |
| title_short | Application of the Insect Cell-Baculovirus Expression Vector System in Adeno-Associated Viral Production |
| title_sort | application of the insect cell baculovirus expression vector system in adeno associated viral production |
| topic | Baculovirus Expression Vector System (BEVS) insect cells Adeno-Associated Virus (AAV) gene therapy large-scale production |
| url | https://www.mdpi.com/2076-3417/14/23/10948 |
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