HIV-1 subtypes and recombinants in Northern Tanzania: distribution of viral quasispecies.
This study analyzed the distribution and prevalence of HIV-1 subtypes, multiplicity of HIV-1 infection, and frequency of inter-subtype recombination among HIV-1-infected female bar and hotel workers in Moshi, Kilimanjaro Region, Tanzania, from 2004 to 2007. The HIV-1 viral sequences spanning the V1-...
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0047605&type=printable |
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| author | Ireen E Kiwelu Vladimir Novitsky Lauren Margolin Jeannie Baca Rachel Manongi Noel Sam John Shao Mary F McLane Saidi H Kapiga M Essex |
| author_facet | Ireen E Kiwelu Vladimir Novitsky Lauren Margolin Jeannie Baca Rachel Manongi Noel Sam John Shao Mary F McLane Saidi H Kapiga M Essex |
| author_sort | Ireen E Kiwelu |
| collection | DOAJ |
| description | This study analyzed the distribution and prevalence of HIV-1 subtypes, multiplicity of HIV-1 infection, and frequency of inter-subtype recombination among HIV-1-infected female bar and hotel workers in Moshi, Kilimanjaro Region, Tanzania, from 2004 to 2007. The HIV-1 viral sequences spanning the V1-C5 region of HIV-1 env gp120 were analyzed from 50 subjects by single genome amplification and sequencing (SGA/S) technique. A total of 1740 sequences were amplified and sequenced from the HIV-1 proviral DNA template. The median env sequences analyzed per subject per two time points was 38 (IQR 28-50) over one year of HIV infection. In a subset of 14 subjects, a total of 239 sequences were obtained from HIV-1 RNA template at the baseline visit. The most prevalent HIV-1 subtypes were A1 (56%) and C (30%), while HIV-1 subtype D and inter-subtype recombinant viruses were found in 6% and 8% of subjects respectively. Transmission of multiple HIV-1 variants was evident in 27% of the subjects infected with pure HIV-1 subtypes A1, C, or D. The HIV-1 inter-subtype recombinants were found in 8% including HIV-1 C/A, D/A, and complex mosaic recombinants. Multiple viral variants were found in two subjects infected with inter-subtype recombinants. One subject harbored quasispecies of both pure HIV-1 A1 and C/A recombinant. The other subject was infected with two complex mosaic inter-subtype recombinant variants belonging to subtype D. HIV-1 multiple infections and ongoing recombination contribute significantly to the genetic diversity of circulating HIV-1 in Tanzania and have important implications for vaccine design and the development of therapeutic strategies. |
| format | Article |
| id | doaj-art-bb358d143f6a41dab58e6c11e95a6007 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-bb358d143f6a41dab58e6c11e95a60072025-08-20T02:30:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01710e4760510.1371/journal.pone.0047605HIV-1 subtypes and recombinants in Northern Tanzania: distribution of viral quasispecies.Ireen E KiweluVladimir NovitskyLauren MargolinJeannie BacaRachel ManongiNoel SamJohn ShaoMary F McLaneSaidi H KapigaM EssexThis study analyzed the distribution and prevalence of HIV-1 subtypes, multiplicity of HIV-1 infection, and frequency of inter-subtype recombination among HIV-1-infected female bar and hotel workers in Moshi, Kilimanjaro Region, Tanzania, from 2004 to 2007. The HIV-1 viral sequences spanning the V1-C5 region of HIV-1 env gp120 were analyzed from 50 subjects by single genome amplification and sequencing (SGA/S) technique. A total of 1740 sequences were amplified and sequenced from the HIV-1 proviral DNA template. The median env sequences analyzed per subject per two time points was 38 (IQR 28-50) over one year of HIV infection. In a subset of 14 subjects, a total of 239 sequences were obtained from HIV-1 RNA template at the baseline visit. The most prevalent HIV-1 subtypes were A1 (56%) and C (30%), while HIV-1 subtype D and inter-subtype recombinant viruses were found in 6% and 8% of subjects respectively. Transmission of multiple HIV-1 variants was evident in 27% of the subjects infected with pure HIV-1 subtypes A1, C, or D. The HIV-1 inter-subtype recombinants were found in 8% including HIV-1 C/A, D/A, and complex mosaic recombinants. Multiple viral variants were found in two subjects infected with inter-subtype recombinants. One subject harbored quasispecies of both pure HIV-1 A1 and C/A recombinant. The other subject was infected with two complex mosaic inter-subtype recombinant variants belonging to subtype D. HIV-1 multiple infections and ongoing recombination contribute significantly to the genetic diversity of circulating HIV-1 in Tanzania and have important implications for vaccine design and the development of therapeutic strategies.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0047605&type=printable |
| spellingShingle | Ireen E Kiwelu Vladimir Novitsky Lauren Margolin Jeannie Baca Rachel Manongi Noel Sam John Shao Mary F McLane Saidi H Kapiga M Essex HIV-1 subtypes and recombinants in Northern Tanzania: distribution of viral quasispecies. PLoS ONE |
| title | HIV-1 subtypes and recombinants in Northern Tanzania: distribution of viral quasispecies. |
| title_full | HIV-1 subtypes and recombinants in Northern Tanzania: distribution of viral quasispecies. |
| title_fullStr | HIV-1 subtypes and recombinants in Northern Tanzania: distribution of viral quasispecies. |
| title_full_unstemmed | HIV-1 subtypes and recombinants in Northern Tanzania: distribution of viral quasispecies. |
| title_short | HIV-1 subtypes and recombinants in Northern Tanzania: distribution of viral quasispecies. |
| title_sort | hiv 1 subtypes and recombinants in northern tanzania distribution of viral quasispecies |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0047605&type=printable |
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