Plasma d-asparagine and the d/l-serine ratio reflect chronic kidney diseases in children regardless of physique
Abstract Biomarkers that accurately reflect renal function are essential in management of chronic kidney diseases (CKD). However, in children, age/physique and medication often alter established renal biomarkers. We studied whether amino acid enantiomers in body fluids correlate with renal function...
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| Format: | Article |
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Springer
2024-06-01
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| Series: | Amino Acids |
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| Online Access: | https://doi.org/10.1007/s00726-024-03400-x |
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| author | Toshimasa Morishita Naoto Nishizaki Sakiko Taniguchi Shinsuke Sakai Tomonori Kimura Masashi Mita Mayu Nakagawa Amane Endo Yoshiyuki Ohtomo Masato Yasui Toshiaki Shimizu Jumpei Sasabe |
| author_facet | Toshimasa Morishita Naoto Nishizaki Sakiko Taniguchi Shinsuke Sakai Tomonori Kimura Masashi Mita Mayu Nakagawa Amane Endo Yoshiyuki Ohtomo Masato Yasui Toshiaki Shimizu Jumpei Sasabe |
| author_sort | Toshimasa Morishita |
| collection | DOAJ |
| description | Abstract Biomarkers that accurately reflect renal function are essential in management of chronic kidney diseases (CKD). However, in children, age/physique and medication often alter established renal biomarkers. We studied whether amino acid enantiomers in body fluids correlate with renal function and whether they are influenced by physique or steroid medication during development. We conducted a prospective study of children 2 to 18 years old with and without CKD. We analyzed associations of serine/asparagine enantiomers in body fluids with major biochemical parameters as well as physique. To study consequences of kidney dysfunction and steroids on serine/asparagine enantiomers, we generated juvenile mice with uninephrectomy, ischemic reperfusion injury, or dexamethasone treatment. We obtained samples from 27 children, of which 12 had CKD due to congenital (n = 7) and perinatal (n = 5) causes. Plasma d-asparagine and the d/l-serine ratio had robust, positive linear associations with serum creatinine and cystatin C, and detected CKD with high sensitivity and specificity, uninfluenced by body size or biochemical parameters. In the animal study, kidney dysfunction increased plasma d-asparagine and the d/l-serine ratio, but dexamethasone treatment did not. Thus, plasma d-asparagine and the d/l-serine ratio can be useful markers for renal function in children. |
| format | Article |
| id | doaj-art-bb2ca5d937b040b1bc2043c0847a2b41 |
| institution | OA Journals |
| issn | 1438-2199 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Springer |
| record_format | Article |
| series | Amino Acids |
| spelling | doaj-art-bb2ca5d937b040b1bc2043c0847a2b412025-08-20T01:57:16ZengSpringerAmino Acids1438-21992024-06-0156111210.1007/s00726-024-03400-xPlasma d-asparagine and the d/l-serine ratio reflect chronic kidney diseases in children regardless of physiqueToshimasa Morishita0Naoto Nishizaki1Sakiko Taniguchi2Shinsuke Sakai3Tomonori Kimura4Masashi Mita5Mayu Nakagawa6Amane Endo7Yoshiyuki Ohtomo8Masato Yasui9Toshiaki Shimizu10Jumpei Sasabe11Department of Pharmacology, Keio University School of MedicineDepartment of Pediatrics, Juntendo University Urayasu HospitalDepartment of Pharmacology, Keio University School of MedicineDepartment of Nephrology, Osaka University School of MedicineDepartment of Nephrology, Osaka University School of MedicineKAGAMI IncDepartment of Pediatrics and Adolescent Medicine, Juntendo University Graduate School of MedicineDepartment of Pediatrics and Adolescent Medicine, Juntendo University Graduate School of MedicineDepartment of Pediatrics, Juntendo University Nerima HospitalDepartment of Pharmacology, Keio University School of MedicineDepartment of Pediatrics and Adolescent Medicine, Juntendo University Graduate School of MedicineDepartment of Pharmacology, Keio University School of MedicineAbstract Biomarkers that accurately reflect renal function are essential in management of chronic kidney diseases (CKD). However, in children, age/physique and medication often alter established renal biomarkers. We studied whether amino acid enantiomers in body fluids correlate with renal function and whether they are influenced by physique or steroid medication during development. We conducted a prospective study of children 2 to 18 years old with and without CKD. We analyzed associations of serine/asparagine enantiomers in body fluids with major biochemical parameters as well as physique. To study consequences of kidney dysfunction and steroids on serine/asparagine enantiomers, we generated juvenile mice with uninephrectomy, ischemic reperfusion injury, or dexamethasone treatment. We obtained samples from 27 children, of which 12 had CKD due to congenital (n = 7) and perinatal (n = 5) causes. Plasma d-asparagine and the d/l-serine ratio had robust, positive linear associations with serum creatinine and cystatin C, and detected CKD with high sensitivity and specificity, uninfluenced by body size or biochemical parameters. In the animal study, kidney dysfunction increased plasma d-asparagine and the d/l-serine ratio, but dexamethasone treatment did not. Thus, plasma d-asparagine and the d/l-serine ratio can be useful markers for renal function in children.https://doi.org/10.1007/s00726-024-03400-xJuvenile kidney functiond-amino acidsBody sizeClinical laboratory test |
| spellingShingle | Toshimasa Morishita Naoto Nishizaki Sakiko Taniguchi Shinsuke Sakai Tomonori Kimura Masashi Mita Mayu Nakagawa Amane Endo Yoshiyuki Ohtomo Masato Yasui Toshiaki Shimizu Jumpei Sasabe Plasma d-asparagine and the d/l-serine ratio reflect chronic kidney diseases in children regardless of physique Amino Acids Juvenile kidney function d-amino acids Body size Clinical laboratory test |
| title | Plasma d-asparagine and the d/l-serine ratio reflect chronic kidney diseases in children regardless of physique |
| title_full | Plasma d-asparagine and the d/l-serine ratio reflect chronic kidney diseases in children regardless of physique |
| title_fullStr | Plasma d-asparagine and the d/l-serine ratio reflect chronic kidney diseases in children regardless of physique |
| title_full_unstemmed | Plasma d-asparagine and the d/l-serine ratio reflect chronic kidney diseases in children regardless of physique |
| title_short | Plasma d-asparagine and the d/l-serine ratio reflect chronic kidney diseases in children regardless of physique |
| title_sort | plasma d asparagine and the d l serine ratio reflect chronic kidney diseases in children regardless of physique |
| topic | Juvenile kidney function d-amino acids Body size Clinical laboratory test |
| url | https://doi.org/10.1007/s00726-024-03400-x |
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