A Laboratory Evaluation for the Potential of Entomopathogenic Fungi against Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae)

The red flour beetle is a very important primary insect pest of wheat worldwide. The experiments were performed to check the virulence of Beauveria bassiana and Isaria fumosorosea by insect dipping method on the adults and 2nd instar of Tribolium castaneum. Conidia were taken from 15 days old fungi...

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Main Authors: Muhammad Akmal, Shoaib Freed, Muhammad Bilal, Muhammad Naeem Malik
Format: Article
Language:English
Published: Hasan Eleroğlu 2020-06-01
Series:Turkish Journal of Agriculture: Food Science and Technology
Subjects:
Online Access:http://www.agrifoodscience.com/index.php/TURJAF/article/view/1460
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author Muhammad Akmal
Shoaib Freed
Muhammad Bilal
Muhammad Naeem Malik
author_facet Muhammad Akmal
Shoaib Freed
Muhammad Bilal
Muhammad Naeem Malik
author_sort Muhammad Akmal
collection DOAJ
description The red flour beetle is a very important primary insect pest of wheat worldwide. The experiments were performed to check the virulence of Beauveria bassiana and Isaria fumosorosea by insect dipping method on the adults and 2nd instar of Tribolium castaneum. Conidia were taken from 15 days old fungi and subsequently four concentrations i.e., 2×108, 3×108, 4×108 and 5×108 spores/ml of both fungi were prepared in 0.05% Tween 80 solution. Minimum 12.5% and maximum 32.5% mortality of adult insects was recorded on 7th day after the treatment at 2×108 and 5×108 spores/ml concentrations of B. bassiana, respectively while on larval stages, minimum 2.5% on 5th day and maximum 80.0% mortality was observed on 7th day post treatment of B. bassiana, respectively. On the other side, minimum 7.5 and maximum 22.5 mortality percentage was noted on 7th day post application of I. fumosorosea, respectively while on immatures minimum 5% on 6th day and highest 70% mortality was noted on 7th day post infection with 2×108 and 5×108 spores/ml of I. fumosorosea, correspondingly. This study showed the effectiveness of insect pathogenic fungi against the important stored grain insect pest and proved to be a positive management strategy.
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publisher Hasan Eleroğlu
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series Turkish Journal of Agriculture: Food Science and Technology
spelling doaj-art-bb287938f336475fab91332a91dd5ff52025-08-20T03:04:38ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2020-06-01861232123510.24925/turjaf.v8i6.1232-1235.14601653A Laboratory Evaluation for the Potential of Entomopathogenic Fungi against Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae)Muhammad Akmal0Shoaib Freed1Muhammad Bilal2Muhammad Naeem Malik3Laboratory of Insect Microbiology and Biotechnology, Department of Entomology, Faculty of Agricultural Sciences and TechnologyLaboratory of Insect Microbiology and Biotechnology, Department of Entomology, Faculty of Agricultural Sciences and TechnologyLaboratory of Insect Microbiology and Biotechnology, Department of Entomology, Faculty of Agricultural Sciences and TechnologyLaboratory of Insect Microbiology and Biotechnology, Department of Entomology, Faculty of Agricultural Sciences and TechnologyThe red flour beetle is a very important primary insect pest of wheat worldwide. The experiments were performed to check the virulence of Beauveria bassiana and Isaria fumosorosea by insect dipping method on the adults and 2nd instar of Tribolium castaneum. Conidia were taken from 15 days old fungi and subsequently four concentrations i.e., 2×108, 3×108, 4×108 and 5×108 spores/ml of both fungi were prepared in 0.05% Tween 80 solution. Minimum 12.5% and maximum 32.5% mortality of adult insects was recorded on 7th day after the treatment at 2×108 and 5×108 spores/ml concentrations of B. bassiana, respectively while on larval stages, minimum 2.5% on 5th day and maximum 80.0% mortality was observed on 7th day post treatment of B. bassiana, respectively. On the other side, minimum 7.5 and maximum 22.5 mortality percentage was noted on 7th day post application of I. fumosorosea, respectively while on immatures minimum 5% on 6th day and highest 70% mortality was noted on 7th day post infection with 2×108 and 5×108 spores/ml of I. fumosorosea, correspondingly. This study showed the effectiveness of insect pathogenic fungi against the important stored grain insect pest and proved to be a positive management strategy.http://www.agrifoodscience.com/index.php/TURJAF/article/view/1460insect pathogenic fungired flour beetlebioassayimmersion methodbeauveria bassiana
spellingShingle Muhammad Akmal
Shoaib Freed
Muhammad Bilal
Muhammad Naeem Malik
A Laboratory Evaluation for the Potential of Entomopathogenic Fungi against Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae)
Turkish Journal of Agriculture: Food Science and Technology
insect pathogenic fungi
red flour beetle
bioassay
immersion method
beauveria bassiana
title A Laboratory Evaluation for the Potential of Entomopathogenic Fungi against Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae)
title_full A Laboratory Evaluation for the Potential of Entomopathogenic Fungi against Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae)
title_fullStr A Laboratory Evaluation for the Potential of Entomopathogenic Fungi against Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae)
title_full_unstemmed A Laboratory Evaluation for the Potential of Entomopathogenic Fungi against Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae)
title_short A Laboratory Evaluation for the Potential of Entomopathogenic Fungi against Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae)
title_sort laboratory evaluation for the potential of entomopathogenic fungi against tribolium castaneum herbst coleoptera tenebrionidae
topic insect pathogenic fungi
red flour beetle
bioassay
immersion method
beauveria bassiana
url http://www.agrifoodscience.com/index.php/TURJAF/article/view/1460
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