Analysis of significantly associated regions and candidate genes for dwarf stem trait in Brassica napus based on BSA-sequencing
[Objective] The study aims to reveal the genetic loci and candidate genes associated with the dwarf trait in Brassica napus, laying a foundation for the improvement of plant height and the breeding of lodging resistant B. napus varieties. [Methods] Rapeseed dwarf stem DW1 was used to cross with ta...
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| Format: | Article |
| Language: | zho |
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Science Press
2024-12-01
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| Series: | Xibei zhiwu xuebao |
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| Online Access: | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240326?st=article_issue |
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| author | ZHAO Weiguo TA Na WANG Hao YANG Zelin |
| author_facet | ZHAO Weiguo TA Na WANG Hao YANG Zelin |
| author_sort | ZHAO Weiguo |
| collection | DOAJ |
| description | [Objective] The study aims to reveal the genetic loci and candidate genes associated with the
dwarf trait in Brassica napus, laying a foundation for the improvement of plant height and the breeding of
lodging resistant B. napus varieties. [Methods] Rapeseed dwarf stem DW1 was used to cross with tall
stem lines WH14 and WH20, respectively, and the F1 and F2 generations were used to analyze the genetic
mechanism behind the dwarf trait. Meanwhile, two mixed DNA pools, one from extremely tall and the
other from dwarf stem lines, constructed from F2 segregated population of dwarf stem DW1 and tall stem
WH20 hybrid combination, were sequenced and analyzed base on BSA-seq technology, and the significant
association regions and candidate genes associated with dwarf trait were identified. [Results] (1) The
dwarf trait of B. napus DW1 was a quality trait and was controlled by one pair of incomplete dominant
genes. (2) Twenty-two genomic regions significantly associated with dwarf trait were identified within the
21.78-23.88 Mb region on chromosome A06, with the maximum interval of 46.71 kb and the minimum interval
of 0.64 kb. (3) Potential candidate genes, including BnaA06g27050D, BnaA06g34100D, BnaA06g34810D,
BnaA06g35080D , and BnaA06g36480D , were identified in significantly associated regions on chromosome
A06, which were related to the regulation of auxin and gibberellin signal transduction, respectively.
[Conclusion] The dwarf trait herein is a quality trait controlled by one pair of incomplete genes. One significantly
associated region was identified in the region from 21.78-23.88 Mb on chromosome A06. Five
candidate genes within the significantly associated regions were identified and involved in the regulation of
plant auxin synthesis and gibberellin signal transduction. |
| format | Article |
| id | doaj-art-bb253113ca6a4274b366133d787eb991 |
| institution | Kabale University |
| issn | 1000-4025 |
| language | zho |
| publishDate | 2024-12-01 |
| publisher | Science Press |
| record_format | Article |
| series | Xibei zhiwu xuebao |
| spelling | doaj-art-bb253113ca6a4274b366133d787eb9912025-02-10T12:31:53ZzhoScience PressXibei zhiwu xuebao1000-40252024-12-0144121890189910.7606/j.issn.1000-4025.20240326Analysis of significantly associated regions and candidate genes for dwarf stem trait in Brassica napus based on BSA-sequencingZHAO Weiguo0TA Na1WANG Hao2YANG Zelin3School of Modern Agriculture and Biotechnology, Ankang University, Ankang, Shaanxi 725000, ChinaSchool of Modern Agriculture and Biotechnology, Ankang University, Ankang, Shaanxi 725000, ChinaThe Rapeseed Hybrid Center of Shaanxi Province, Yangling, Shaanxi 712100, ChinaSchool of Modern Agriculture and Biotechnology, Ankang University, Ankang, Shaanxi 725000, China[Objective] The study aims to reveal the genetic loci and candidate genes associated with the dwarf trait in Brassica napus, laying a foundation for the improvement of plant height and the breeding of lodging resistant B. napus varieties. [Methods] Rapeseed dwarf stem DW1 was used to cross with tall stem lines WH14 and WH20, respectively, and the F1 and F2 generations were used to analyze the genetic mechanism behind the dwarf trait. Meanwhile, two mixed DNA pools, one from extremely tall and the other from dwarf stem lines, constructed from F2 segregated population of dwarf stem DW1 and tall stem WH20 hybrid combination, were sequenced and analyzed base on BSA-seq technology, and the significant association regions and candidate genes associated with dwarf trait were identified. [Results] (1) The dwarf trait of B. napus DW1 was a quality trait and was controlled by one pair of incomplete dominant genes. (2) Twenty-two genomic regions significantly associated with dwarf trait were identified within the 21.78-23.88 Mb region on chromosome A06, with the maximum interval of 46.71 kb and the minimum interval of 0.64 kb. (3) Potential candidate genes, including BnaA06g27050D, BnaA06g34100D, BnaA06g34810D, BnaA06g35080D , and BnaA06g36480D , were identified in significantly associated regions on chromosome A06, which were related to the regulation of auxin and gibberellin signal transduction, respectively. [Conclusion] The dwarf trait herein is a quality trait controlled by one pair of incomplete genes. One significantly associated region was identified in the region from 21.78-23.88 Mb on chromosome A06. Five candidate genes within the significantly associated regions were identified and involved in the regulation of plant auxin synthesis and gibberellin signal transduction.http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240326?st=article_issuebrassica napus l.dwarf traitbsa sequencingsignificant correlation regionscandidate genes |
| spellingShingle | ZHAO Weiguo TA Na WANG Hao YANG Zelin Analysis of significantly associated regions and candidate genes for dwarf stem trait in Brassica napus based on BSA-sequencing Xibei zhiwu xuebao brassica napus l. dwarf trait bsa sequencing significant correlation regions candidate genes |
| title | Analysis of significantly associated regions and candidate genes for dwarf stem trait in Brassica napus based on BSA-sequencing |
| title_full | Analysis of significantly associated regions and candidate genes for dwarf stem trait in Brassica napus based on BSA-sequencing |
| title_fullStr | Analysis of significantly associated regions and candidate genes for dwarf stem trait in Brassica napus based on BSA-sequencing |
| title_full_unstemmed | Analysis of significantly associated regions and candidate genes for dwarf stem trait in Brassica napus based on BSA-sequencing |
| title_short | Analysis of significantly associated regions and candidate genes for dwarf stem trait in Brassica napus based on BSA-sequencing |
| title_sort | analysis of significantly associated regions and candidate genes for dwarf stem trait in brassica napus based on bsa sequencing |
| topic | brassica napus l. dwarf trait bsa sequencing significant correlation regions candidate genes |
| url | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240326?st=article_issue |
| work_keys_str_mv | AT zhaoweiguo analysisofsignificantlyassociatedregionsandcandidategenesfordwarfstemtraitinbrassicanapusbasedonbsasequencing AT tana analysisofsignificantlyassociatedregionsandcandidategenesfordwarfstemtraitinbrassicanapusbasedonbsasequencing AT wanghao analysisofsignificantlyassociatedregionsandcandidategenesfordwarfstemtraitinbrassicanapusbasedonbsasequencing AT yangzelin analysisofsignificantlyassociatedregionsandcandidategenesfordwarfstemtraitinbrassicanapusbasedonbsasequencing |