In Vitro Inhibitory Effect of Clove Essential Oil and Its Two Active Principles on Tooth Decalcification by Apple Juice
The dental erosion or decalcification of enamel is a significant clinical problem. Apple acidic beverages are thought to increase the potential for dental erosion. The purpose of this in vitro study was to investigate the effect of clove essential oil (CEO) and its active principles on tooth decalci...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2012/759618 |
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| author | Charu M. Marya Gunjan Satija Avinash J. Ruchi Nagpal Rohtash Kapoor Aijaz Ahmad |
| author_facet | Charu M. Marya Gunjan Satija Avinash J. Ruchi Nagpal Rohtash Kapoor Aijaz Ahmad |
| author_sort | Charu M. Marya |
| collection | DOAJ |
| description | The dental erosion or decalcification of enamel is a significant clinical problem. Apple acidic beverages are thought to increase the potential for dental erosion. The purpose of this in vitro study was to investigate the effect of clove essential oil (CEO) and its active principles on tooth decalcification of apple juices. On GC-MS analysis, CEO showed a high content of eugenol (58.29%) and eugenyl acetate (19.10%). Teeth specimens were randomly divided into 5 treatment groups: control, CEO, eugenol, eugenyl-acetate, and fluoride. The specimens were exposed for 24 h and were analyzed for calcium contents using Inductively Coupled Plasma with Optical Emission Spectroscopy (ICP-OES). Data were analyzed using student t-test (𝑃<0.05). CEO, eugenol, and eugenyl-acetate significantly decreased the decalcification of tooth by the apple juice to only 17, 24, and 21 mgL−1, respectively. Hemolytic activity on human erythrocytes was studied to exclude the possibility of further associated cytotoxicity. It was observed that the CEO and its two lead molecules inhibit the decalcification and/or promote the remineralization caused by the apple juices. The effect of the test compounds appears to be distinct like that of fluoride treatment. CEO may, therefore, serve to be a promising adjunct to fluoride in the treatment of root caries during minimally invasive therapy. |
| format | Article |
| id | doaj-art-bb2390ba69114aacaab0bc64dcc56ecb |
| institution | OA Journals |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-bb2390ba69114aacaab0bc64dcc56ecb2025-08-20T02:19:21ZengWileyInternational Journal of Dentistry1687-87281687-87362012-01-01201210.1155/2012/759618759618In Vitro Inhibitory Effect of Clove Essential Oil and Its Two Active Principles on Tooth Decalcification by Apple JuiceCharu M. Marya0Gunjan Satija1Avinash J.2Ruchi Nagpal3Rohtash Kapoor4Aijaz Ahmad5Department of Public Health Dentistry, Sudha Rustagi College of Dental Sciences and Research, Faridabad, Haryana, IndiaDepartment of Public Health Dentistry, Sudha Rustagi College of Dental Sciences and Research, Faridabad, Haryana, IndiaDepartment of Public Health Dentistry, Sudha Rustagi College of Dental Sciences and Research, Faridabad, Haryana, IndiaDepartment of Public Health Dentistry, Sudha Rustagi College of Dental Sciences and Research, Faridabad, Haryana, IndiaDepartment of Prosthodontics, Maharaja Ganga Singh Dental and Research College, Sri Ganga Nagar, Rajasthan, IndiaDepartment of Biosciences, Jamia Millia Islamia, New Delhi, IndiaThe dental erosion or decalcification of enamel is a significant clinical problem. Apple acidic beverages are thought to increase the potential for dental erosion. The purpose of this in vitro study was to investigate the effect of clove essential oil (CEO) and its active principles on tooth decalcification of apple juices. On GC-MS analysis, CEO showed a high content of eugenol (58.29%) and eugenyl acetate (19.10%). Teeth specimens were randomly divided into 5 treatment groups: control, CEO, eugenol, eugenyl-acetate, and fluoride. The specimens were exposed for 24 h and were analyzed for calcium contents using Inductively Coupled Plasma with Optical Emission Spectroscopy (ICP-OES). Data were analyzed using student t-test (𝑃<0.05). CEO, eugenol, and eugenyl-acetate significantly decreased the decalcification of tooth by the apple juice to only 17, 24, and 21 mgL−1, respectively. Hemolytic activity on human erythrocytes was studied to exclude the possibility of further associated cytotoxicity. It was observed that the CEO and its two lead molecules inhibit the decalcification and/or promote the remineralization caused by the apple juices. The effect of the test compounds appears to be distinct like that of fluoride treatment. CEO may, therefore, serve to be a promising adjunct to fluoride in the treatment of root caries during minimally invasive therapy.http://dx.doi.org/10.1155/2012/759618 |
| spellingShingle | Charu M. Marya Gunjan Satija Avinash J. Ruchi Nagpal Rohtash Kapoor Aijaz Ahmad In Vitro Inhibitory Effect of Clove Essential Oil and Its Two Active Principles on Tooth Decalcification by Apple Juice International Journal of Dentistry |
| title | In Vitro Inhibitory Effect of Clove Essential Oil and Its Two Active Principles on Tooth Decalcification by Apple Juice |
| title_full | In Vitro Inhibitory Effect of Clove Essential Oil and Its Two Active Principles on Tooth Decalcification by Apple Juice |
| title_fullStr | In Vitro Inhibitory Effect of Clove Essential Oil and Its Two Active Principles on Tooth Decalcification by Apple Juice |
| title_full_unstemmed | In Vitro Inhibitory Effect of Clove Essential Oil and Its Two Active Principles on Tooth Decalcification by Apple Juice |
| title_short | In Vitro Inhibitory Effect of Clove Essential Oil and Its Two Active Principles on Tooth Decalcification by Apple Juice |
| title_sort | in vitro inhibitory effect of clove essential oil and its two active principles on tooth decalcification by apple juice |
| url | http://dx.doi.org/10.1155/2012/759618 |
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