Emerging trends in prime editing for precision genome editing
Abstract Prime editing is an advanced genome editing technology that enables precise genetic modifications without inducing double-strand breaks or requiring donor DNA templates. Prime editing has rapidly become a versatile tool, supporting a wide range of genetic modifications, including point muta...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2025-07-01
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| Series: | Experimental and Molecular Medicine |
| Online Access: | https://doi.org/10.1038/s12276-025-01463-8 |
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| _version_ | 1849767496731590656 |
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| author | Jaesuk Lee Jiyeon Kweon Yongsub Kim |
| author_facet | Jaesuk Lee Jiyeon Kweon Yongsub Kim |
| author_sort | Jaesuk Lee |
| collection | DOAJ |
| description | Abstract Prime editing is an advanced genome editing technology that enables precise genetic modifications without inducing double-strand breaks or requiring donor DNA templates. Prime editing has rapidly become a versatile tool, supporting a wide range of genetic modifications, including point mutations, insertions and deletions. Here we examine the evolution of prime editing technologies, detailing advancements from the initial prime editing systems to recent innovations that enhance editing efficiency. Through structural modifications and improved delivery methods, prime editing has expanded its applicability across eukaryotic systems. By enabling access to previously challenging mutations, prime editing opens new avenues for therapeutic development and precision genetic research, with efficiency, specificity and accessibility expected to shape its future impact in genome engineering. |
| format | Article |
| id | doaj-art-bb2052b55d9e4ebdb0ab7bec6b46b627 |
| institution | DOAJ |
| issn | 2092-6413 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Experimental and Molecular Medicine |
| spelling | doaj-art-bb2052b55d9e4ebdb0ab7bec6b46b6272025-08-20T03:04:10ZengNature Publishing GroupExperimental and Molecular Medicine2092-64132025-07-015771381139110.1038/s12276-025-01463-8Emerging trends in prime editing for precision genome editingJaesuk Lee0Jiyeon Kweon1Yongsub Kim2nSAGE Inc.Department of Cell and Genetic Engineering, BK21 Project, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of MedicineDepartment of Cell and Genetic Engineering, BK21 Project, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of MedicineAbstract Prime editing is an advanced genome editing technology that enables precise genetic modifications without inducing double-strand breaks or requiring donor DNA templates. Prime editing has rapidly become a versatile tool, supporting a wide range of genetic modifications, including point mutations, insertions and deletions. Here we examine the evolution of prime editing technologies, detailing advancements from the initial prime editing systems to recent innovations that enhance editing efficiency. Through structural modifications and improved delivery methods, prime editing has expanded its applicability across eukaryotic systems. By enabling access to previously challenging mutations, prime editing opens new avenues for therapeutic development and precision genetic research, with efficiency, specificity and accessibility expected to shape its future impact in genome engineering.https://doi.org/10.1038/s12276-025-01463-8 |
| spellingShingle | Jaesuk Lee Jiyeon Kweon Yongsub Kim Emerging trends in prime editing for precision genome editing Experimental and Molecular Medicine |
| title | Emerging trends in prime editing for precision genome editing |
| title_full | Emerging trends in prime editing for precision genome editing |
| title_fullStr | Emerging trends in prime editing for precision genome editing |
| title_full_unstemmed | Emerging trends in prime editing for precision genome editing |
| title_short | Emerging trends in prime editing for precision genome editing |
| title_sort | emerging trends in prime editing for precision genome editing |
| url | https://doi.org/10.1038/s12276-025-01463-8 |
| work_keys_str_mv | AT jaesuklee emergingtrendsinprimeeditingforprecisiongenomeediting AT jiyeonkweon emergingtrendsinprimeeditingforprecisiongenomeediting AT yongsubkim emergingtrendsinprimeeditingforprecisiongenomeediting |