Selenium compartmentalization in arbuscular mycorrhizal fungi associated with brachiaria grass
ABSTRACT Selenium (Se) is essential for humans and other animals and beneficial to plants. However, high Se concentrations cause toxicity in living organisms. Arbuscular mycorrhizae are a symbiotic interaction between arbuscular mycorrhizal fungi (AMF) and plants that can alleviate metal phytotoxici...
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Universidade Federal de Lavras
2025-05-01
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| Series: | Ciência e Agrotecnologia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542025000100215&lng=en&tlng=en |
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| author | Soraya Bamberg Ananias Arnon Afonso de Souza Cardoso Ediu Carlos da Silva Júnior Cynthia de Oliveira Teotônio Soares de Carvalho Aline Oliveira Silva Luiz Roberto Guimarães Guilherme Marco Aurélio Carbone Carneiro |
| author_facet | Soraya Bamberg Ananias Arnon Afonso de Souza Cardoso Ediu Carlos da Silva Júnior Cynthia de Oliveira Teotônio Soares de Carvalho Aline Oliveira Silva Luiz Roberto Guimarães Guilherme Marco Aurélio Carbone Carneiro |
| author_sort | Soraya Bamberg Ananias |
| collection | DOAJ |
| description | ABSTRACT Selenium (Se) is essential for humans and other animals and beneficial to plants. However, high Se concentrations cause toxicity in living organisms. Arbuscular mycorrhizae are a symbiotic interaction between arbuscular mycorrhizal fungi (AMF) and plants that can alleviate metal phytotoxicity. This study used synchrotron X-ray fluorescence (S-XRF) microanalyses to identify Se compartmentalization in AMF structures as a possible protection mechanism against Se phytotoxicity. Our experiments investigated the Se exposure in brachiaria (Urochloa decumbens) plants under AMF inoculation (pot experiment) and AMF spores (Petri dish experiment). In the pot experiment, the treatments consisted of five doses of Se in soil (0, 2, 4, 6, and 8 mg kg-1) combined with four inoculation conditions (Acaulospora morrowiae, Dentiscutata heterogama, Rhizophagus clarus, and not inoculated). In the Petri dish experiment, seven spores of D. heterogama were grown in an agar medium with the same Se doses described previously. The inoculated plants showed shoot dry matter production 26% higher than the not inoculated under 8 mg kg-1 Se. The R. clarus inoculation decreased the Se concentration in shoots by 37% compared to the other conditions under the highest Se dose. In addition, the S-XRF analysis identified a R. clarus vesicle inside a root fragment, showing a Se concentration ten times higher than in root tissues. The S-XRF analysis identified 35 mg kg-1 Se in spores of D. heterogama cultivated with 8 mg L-1 Se. The results indicated that AMF inoculation can protect brachiaria against Se toxicity due to Se compartmentalization in vesicles and spores. |
| format | Article |
| id | doaj-art-bb0e21ba6d6e4abe8b10fcdd70327b17 |
| institution | OA Journals |
| issn | 1981-1829 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Universidade Federal de Lavras |
| record_format | Article |
| series | Ciência e Agrotecnologia |
| spelling | doaj-art-bb0e21ba6d6e4abe8b10fcdd70327b172025-08-20T01:55:08ZengUniversidade Federal de LavrasCiência e Agrotecnologia1981-18292025-05-014910.1590/1413-7054202549022224Selenium compartmentalization in arbuscular mycorrhizal fungi associated with brachiaria grassSoraya Bamberg Ananiashttps://orcid.org/0000-0003-3515-6104Arnon Afonso de Souza Cardosohttps://orcid.org/0000-0002-4358-2026Ediu Carlos da Silva Júniorhttps://orcid.org/0000-0001-8592-2204Cynthia de Oliveirahttps://orcid.org/0000-0002-8030-4025Teotônio Soares de Carvalhohttps://orcid.org/0000-0001-6631-5177Aline Oliveira Silvahttps://orcid.org/0000-0003-0317-4797Luiz Roberto Guimarães Guilhermehttps://orcid.org/0000-0002-5387-6028Marco Aurélio Carbone Carneirohttps://orcid.org/0000-0003-4349-3071ABSTRACT Selenium (Se) is essential for humans and other animals and beneficial to plants. However, high Se concentrations cause toxicity in living organisms. Arbuscular mycorrhizae are a symbiotic interaction between arbuscular mycorrhizal fungi (AMF) and plants that can alleviate metal phytotoxicity. This study used synchrotron X-ray fluorescence (S-XRF) microanalyses to identify Se compartmentalization in AMF structures as a possible protection mechanism against Se phytotoxicity. Our experiments investigated the Se exposure in brachiaria (Urochloa decumbens) plants under AMF inoculation (pot experiment) and AMF spores (Petri dish experiment). In the pot experiment, the treatments consisted of five doses of Se in soil (0, 2, 4, 6, and 8 mg kg-1) combined with four inoculation conditions (Acaulospora morrowiae, Dentiscutata heterogama, Rhizophagus clarus, and not inoculated). In the Petri dish experiment, seven spores of D. heterogama were grown in an agar medium with the same Se doses described previously. The inoculated plants showed shoot dry matter production 26% higher than the not inoculated under 8 mg kg-1 Se. The R. clarus inoculation decreased the Se concentration in shoots by 37% compared to the other conditions under the highest Se dose. In addition, the S-XRF analysis identified a R. clarus vesicle inside a root fragment, showing a Se concentration ten times higher than in root tissues. The S-XRF analysis identified 35 mg kg-1 Se in spores of D. heterogama cultivated with 8 mg L-1 Se. The results indicated that AMF inoculation can protect brachiaria against Se toxicity due to Se compartmentalization in vesicles and spores.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542025000100215&lng=en&tlng=enUrochloa decumbensarbuscular mycorrhizaeselenatephytotoxicity |
| spellingShingle | Soraya Bamberg Ananias Arnon Afonso de Souza Cardoso Ediu Carlos da Silva Júnior Cynthia de Oliveira Teotônio Soares de Carvalho Aline Oliveira Silva Luiz Roberto Guimarães Guilherme Marco Aurélio Carbone Carneiro Selenium compartmentalization in arbuscular mycorrhizal fungi associated with brachiaria grass Ciência e Agrotecnologia Urochloa decumbens arbuscular mycorrhizae selenate phytotoxicity |
| title | Selenium compartmentalization in arbuscular mycorrhizal fungi associated with brachiaria grass |
| title_full | Selenium compartmentalization in arbuscular mycorrhizal fungi associated with brachiaria grass |
| title_fullStr | Selenium compartmentalization in arbuscular mycorrhizal fungi associated with brachiaria grass |
| title_full_unstemmed | Selenium compartmentalization in arbuscular mycorrhizal fungi associated with brachiaria grass |
| title_short | Selenium compartmentalization in arbuscular mycorrhizal fungi associated with brachiaria grass |
| title_sort | selenium compartmentalization in arbuscular mycorrhizal fungi associated with brachiaria grass |
| topic | Urochloa decumbens arbuscular mycorrhizae selenate phytotoxicity |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542025000100215&lng=en&tlng=en |
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