A technique to increase protein yield in a rabbit reticulocyte lysate translation system
Rabbit reticulocyte lysate (RRL) is a mammalian cell-free system for protein production. However, one of the limitations of this system is its low protein yield. Inclusion of recombinant virus proteins and specific viral structures on target mRNA could enhance protein production in RRL. Here we show...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2014-01-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/000114125 |
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| author | Maria Anastasina Ilya Terenin Sarah J Butcher Denis E Kainov |
| author_facet | Maria Anastasina Ilya Terenin Sarah J Butcher Denis E Kainov |
| author_sort | Maria Anastasina |
| collection | DOAJ |
| description | Rabbit reticulocyte lysate (RRL) is a mammalian cell-free system for protein production. However, one of the limitations of this system is its low protein yield. Inclusion of recombinant virus proteins and specific viral structures on target mRNA could enhance protein production in RRL. Here we show that simultaneous addition of influenza A virus NS1 protein and inclusion of the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) in the target mRNA facilitate translation initiation and increase protein yield over 10-fold, improving the translation capacity of RRL. |
| format | Article |
| id | doaj-art-bb009252dbf840c8b4395757c8d89231 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-bb009252dbf840c8b4395757c8d892312025-08-20T02:25:55ZengTaylor & Francis GroupBioTechniques0736-62051940-98182014-01-01561363910.2144/000114125A technique to increase protein yield in a rabbit reticulocyte lysate translation systemMaria Anastasina0Ilya Terenin1Sarah J Butcher2Denis E Kainov31Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Finland2Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russian Federation3Institute of Biotechnology, University of Helsinki, Finland1Institute for Molecular Medicine Finland (FIMM), University of Helsinki, FinlandRabbit reticulocyte lysate (RRL) is a mammalian cell-free system for protein production. However, one of the limitations of this system is its low protein yield. Inclusion of recombinant virus proteins and specific viral structures on target mRNA could enhance protein production in RRL. Here we show that simultaneous addition of influenza A virus NS1 protein and inclusion of the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) in the target mRNA facilitate translation initiation and increase protein yield over 10-fold, improving the translation capacity of RRL.https://www.future-science.com/doi/10.2144/000114125cell-free translationrabbit reticulocyte lysateinfluenza A virusnon-structural proteinEMCV IRES |
| spellingShingle | Maria Anastasina Ilya Terenin Sarah J Butcher Denis E Kainov A technique to increase protein yield in a rabbit reticulocyte lysate translation system BioTechniques cell-free translation rabbit reticulocyte lysate influenza A virus non-structural protein EMCV IRES |
| title | A technique to increase protein yield in a rabbit reticulocyte lysate translation system |
| title_full | A technique to increase protein yield in a rabbit reticulocyte lysate translation system |
| title_fullStr | A technique to increase protein yield in a rabbit reticulocyte lysate translation system |
| title_full_unstemmed | A technique to increase protein yield in a rabbit reticulocyte lysate translation system |
| title_short | A technique to increase protein yield in a rabbit reticulocyte lysate translation system |
| title_sort | technique to increase protein yield in a rabbit reticulocyte lysate translation system |
| topic | cell-free translation rabbit reticulocyte lysate influenza A virus non-structural protein EMCV IRES |
| url | https://www.future-science.com/doi/10.2144/000114125 |
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