Immunohistochemical Differentiation between Western and East Asian Types of CagA-Positive Helicobacter pylori in Gastric Biopsy Samples
Background. Cag A-positive Helicobacter pylori isolated from human gastric mucosa is categorized as a Western or East Asian allele-type based on whether the cagA gene encodes an EPIYA-C or EPIYA-D motif. We aimed to differentiate between the 2 types of H. pylori by immunohistochemistry (IHC) using f...
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Wiley
2022-01-01
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| Series: | Canadian Journal of Gastroenterology and Hepatology |
| Online Access: | http://dx.doi.org/10.1155/2022/1371089 |
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| author | Daisuke Kobayashi Keisuke Uchida Asuka Furukawa Takashi Ito Luis Masuo Maruta Heinrich Bender Kohnert Seidler Aloisio Felipe-Silva Masaki Sekine Noboru Ando Yuka Toyama Yusuke Chino Keiko Miura Kurara Yamamoto Takumi Akashi Yoshinobu Eishi Kenichi Ohashi |
| author_facet | Daisuke Kobayashi Keisuke Uchida Asuka Furukawa Takashi Ito Luis Masuo Maruta Heinrich Bender Kohnert Seidler Aloisio Felipe-Silva Masaki Sekine Noboru Ando Yuka Toyama Yusuke Chino Keiko Miura Kurara Yamamoto Takumi Akashi Yoshinobu Eishi Kenichi Ohashi |
| author_sort | Daisuke Kobayashi |
| collection | DOAJ |
| description | Background. Cag A-positive Helicobacter pylori isolated from human gastric mucosa is categorized as a Western or East Asian allele-type based on whether the cagA gene encodes an EPIYA-C or EPIYA-D motif. We aimed to differentiate between the 2 types of H. pylori by immunohistochemistry (IHC) using formalin-fixed paraffin-embedded (FFPE) gastric biopsy samples. Materials and Methods. We developed 2 monoclonal antibodies (mAbs) that detect either the EPIYA-C or EPIYA-D motif of the H. pylori CagA protein by IHC using FFPE tissues. FFPE tissue sections from 30 Japanese and 39 Brazilian gastric biopsy samples with H. pylori infection confirmed by Giemsa staining (moderate/severe in the Sydney classification system) were examined by IHC with the novel mAbs followed by polymerase chain reaction (PCR) for EPIYA-C or EPIYA-D using DNA extracted from adjacent tissue sections. Results. Differentiation among Western and East Asian types and CagA-negative H. pylori was successful in most (97%) samples by IHC with the novel mAbs and commercially available mAbs that react with a species-specific lipopolysaccharide or a common CagA motif of H. pylori. The detection status of EPIYA-C/D motifs by IHC with the novel mAbs was consistent with the PCR results in 61 (88%) of 69 samples: EPIYA-C(+)/D(−) in zero Japanese and 26 Brazilian samples, EPIYA-C(−)/D(+) in 26 Japanese and 1 Brazilian sample, and EPIYA-C(−)/D(−) in 1 Japanese and 7 Brazilian samples. The detection sensitivity and specificity of IHC with each novel mAb compared with the PCR results were, respectively, 84% and 97% for EPIYA-C, and 97% and 95% for EPIYA-D. Conclusions. The novel mAbs specific to each EPIYA-C or EPIYA-D motif differentiated between Western and East Asian types of CagA-positive H. pylori by IHC using FFPE tissues. Applying these novel mAbs to large numbers of archived pathology samples will contribute to elucidating the association of these allele types with gastric cancer. |
| format | Article |
| id | doaj-art-baf9909d3f184b289852676df740800d |
| institution | OA Journals |
| issn | 2291-2797 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
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| series | Canadian Journal of Gastroenterology and Hepatology |
| spelling | doaj-art-baf9909d3f184b289852676df740800d2025-08-20T02:18:42ZengWileyCanadian Journal of Gastroenterology and Hepatology2291-27972022-01-01202210.1155/2022/1371089Immunohistochemical Differentiation between Western and East Asian Types of CagA-Positive Helicobacter pylori in Gastric Biopsy SamplesDaisuke Kobayashi0Keisuke Uchida1Asuka Furukawa2Takashi Ito3Luis Masuo Maruta4Heinrich Bender Kohnert Seidler5Aloisio Felipe-Silva6Masaki Sekine7Noboru Ando8Yuka Toyama9Yusuke Chino10Keiko Miura11Kurara Yamamoto12Takumi Akashi13Yoshinobu Eishi14Kenichi Ohashi15Department of Human PathologyDivision of Surgical PathologyDepartment of Human PathologyDepartment of Human PathologyUniversidade de Sao Paulo (USP)Laboratório BrasilienseUniversidade de Sao Paulo (USP)Division of Surgical PathologyDivision of Surgical PathologyDivision of Surgical PathologyDivision of Surgical PathologyDivision of Surgical PathologyDepartment of Human PathologyDivision of Surgical PathologyDepartment of Human PathologyDepartment of Human PathologyBackground. Cag A-positive Helicobacter pylori isolated from human gastric mucosa is categorized as a Western or East Asian allele-type based on whether the cagA gene encodes an EPIYA-C or EPIYA-D motif. We aimed to differentiate between the 2 types of H. pylori by immunohistochemistry (IHC) using formalin-fixed paraffin-embedded (FFPE) gastric biopsy samples. Materials and Methods. We developed 2 monoclonal antibodies (mAbs) that detect either the EPIYA-C or EPIYA-D motif of the H. pylori CagA protein by IHC using FFPE tissues. FFPE tissue sections from 30 Japanese and 39 Brazilian gastric biopsy samples with H. pylori infection confirmed by Giemsa staining (moderate/severe in the Sydney classification system) were examined by IHC with the novel mAbs followed by polymerase chain reaction (PCR) for EPIYA-C or EPIYA-D using DNA extracted from adjacent tissue sections. Results. Differentiation among Western and East Asian types and CagA-negative H. pylori was successful in most (97%) samples by IHC with the novel mAbs and commercially available mAbs that react with a species-specific lipopolysaccharide or a common CagA motif of H. pylori. The detection status of EPIYA-C/D motifs by IHC with the novel mAbs was consistent with the PCR results in 61 (88%) of 69 samples: EPIYA-C(+)/D(−) in zero Japanese and 26 Brazilian samples, EPIYA-C(−)/D(+) in 26 Japanese and 1 Brazilian sample, and EPIYA-C(−)/D(−) in 1 Japanese and 7 Brazilian samples. The detection sensitivity and specificity of IHC with each novel mAb compared with the PCR results were, respectively, 84% and 97% for EPIYA-C, and 97% and 95% for EPIYA-D. Conclusions. The novel mAbs specific to each EPIYA-C or EPIYA-D motif differentiated between Western and East Asian types of CagA-positive H. pylori by IHC using FFPE tissues. Applying these novel mAbs to large numbers of archived pathology samples will contribute to elucidating the association of these allele types with gastric cancer.http://dx.doi.org/10.1155/2022/1371089 |
| spellingShingle | Daisuke Kobayashi Keisuke Uchida Asuka Furukawa Takashi Ito Luis Masuo Maruta Heinrich Bender Kohnert Seidler Aloisio Felipe-Silva Masaki Sekine Noboru Ando Yuka Toyama Yusuke Chino Keiko Miura Kurara Yamamoto Takumi Akashi Yoshinobu Eishi Kenichi Ohashi Immunohistochemical Differentiation between Western and East Asian Types of CagA-Positive Helicobacter pylori in Gastric Biopsy Samples Canadian Journal of Gastroenterology and Hepatology |
| title | Immunohistochemical Differentiation between Western and East Asian Types of CagA-Positive Helicobacter pylori in Gastric Biopsy Samples |
| title_full | Immunohistochemical Differentiation between Western and East Asian Types of CagA-Positive Helicobacter pylori in Gastric Biopsy Samples |
| title_fullStr | Immunohistochemical Differentiation between Western and East Asian Types of CagA-Positive Helicobacter pylori in Gastric Biopsy Samples |
| title_full_unstemmed | Immunohistochemical Differentiation between Western and East Asian Types of CagA-Positive Helicobacter pylori in Gastric Biopsy Samples |
| title_short | Immunohistochemical Differentiation between Western and East Asian Types of CagA-Positive Helicobacter pylori in Gastric Biopsy Samples |
| title_sort | immunohistochemical differentiation between western and east asian types of caga positive helicobacter pylori in gastric biopsy samples |
| url | http://dx.doi.org/10.1155/2022/1371089 |
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