Quantitative analysis of ethylene precursor ACC in plant samples by liquid chromatography-tandem mass spectrometry
Abstract Background Ethylene (C2H4) is a gaseous phytohormone that regulates various plant physiological processes and mediates the responses of the plants to various environmental stresses. 1-Aminocyclopropane-1-carboxylic acid (ACC) is the direct precursor of the phytohormone ethylene, and is also...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-07-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-025-06943-7 |
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| Summary: | Abstract Background Ethylene (C2H4) is a gaseous phytohormone that regulates various plant physiological processes and mediates the responses of the plants to various environmental stresses. 1-Aminocyclopropane-1-carboxylic acid (ACC) is the direct precursor of the phytohormone ethylene, and is also considered as a plant growth regulator. Accurate quantification of ACC is critically important in investigating its function. However, it remains challenging to accurately quantify ACC in plant tissues because it is a small, electroneutral molecule with very low concentrations. Methods An easy, cost-saving, and highly efficient quantitative method for ACC in plant tissues was set up by liquid-liquid micro-extraction (LLME) purification with green solvent ethyl-acetate, and the precise control of the mobile phase entering into the mass spectrometer combined with the ultra-high performance liquid chromatography electrospray ionization-triple quadrupole mass spectrometer (UHPLC-ESI-MS/MS). Results The contents of ACC in 10 mg of different fresh fruits were detected. The established method had limit of detection (LOD) (2.5 pg), matrix effect (ME) (92.6%), good precision (3.54%), recovery rate (95.82%), and a good linear relationship within the range of 0.5 to 1500 ng.mL−1 (R2 = 0.9998). Conclusion We have developed an easy, sensitive, and cost-saving quantitative method for ACC in plant tissues without derivatization which is useful for the precise quantification of ACC in plant tissues. |
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| ISSN: | 1471-2229 |