Double Stokes polarimetric microscopy for chiral fibrillar aggregates
Abstract Second harmonic generation (SHG) microscopy is a powerful tool for imaging collagen and other noncentrosymmetric fibrillar structures in biological tissue. Polarimetric SHG measurements provide ultrastructural information about the fibrillar organization in a focal volume (voxel). We presen...
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| Format: | Article |
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Nature Portfolio
2025-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-86893-0 |
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| author | Viktoras Mazeika Kamdin Mirsanaye Leonardo Uribe Castaño Serguei Krouglov Mehdi Alizadeh Mykolas Maciulis Lukas Kontenis Vitalijus Karabanovas Virginijus Barzda |
| author_facet | Viktoras Mazeika Kamdin Mirsanaye Leonardo Uribe Castaño Serguei Krouglov Mehdi Alizadeh Mykolas Maciulis Lukas Kontenis Vitalijus Karabanovas Virginijus Barzda |
| author_sort | Viktoras Mazeika |
| collection | DOAJ |
| description | Abstract Second harmonic generation (SHG) microscopy is a powerful tool for imaging collagen and other noncentrosymmetric fibrillar structures in biological tissue. Polarimetric SHG measurements provide ultrastructural information about the fibrillar organization in a focal volume (voxel). We present a reduced nonlinear polarimetry method named double Stokes polarimetry (DSP) for quick characterization of chiral $$C_6$$ C 6 symmetry fibers without data fitting that simplifies and speeds up the polarimetric analysis. The method is based on double Stokes-Mueller polarimetry and uses linear and circular incident and outgoing polarization states. The analytical expressions of DSP polarimetric parameters are defined in terms of conventional SHG Stokes vector components. A complex chiral susceptibility (CCS) model is assumed to derive expressions of ultrastructural parameters consisting of the magnitude and phase of molecular complex-valued chiral susceptibility ratio, real-valued achiral ratio, and fiber orientation in a voxel. The ultrastructural parameters are expressed in terms of directly measurable DSP polarimetric parameters. DSP is validated with rat tail tendons sectioned at different orientations. DSP can be applied to investigate the origin of chiral complex-valued susceptibility of collagen, to study modifications of collagen in cancerous tissue, and to map ultrastructural parameters of large areas for whole-slide histopathology. |
| format | Article |
| id | doaj-art-baec0cf86b7f44eca66a0e0b0a86a0da |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-baec0cf86b7f44eca66a0e0b0a86a0da2025-02-09T12:29:02ZengNature PortfolioScientific Reports2045-23222025-02-0115111410.1038/s41598-025-86893-0Double Stokes polarimetric microscopy for chiral fibrillar aggregatesViktoras Mazeika0Kamdin Mirsanaye1Leonardo Uribe Castaño2Serguei Krouglov3Mehdi Alizadeh4Mykolas Maciulis5Lukas Kontenis6Vitalijus Karabanovas7Virginijus Barzda8Institute of Biosciences, Life Sciences Center, Vilnius UniversityDepartment of Chemical and Physical Sciences, University of Toronto MississaugaDepartment of Chemical and Physical Sciences, University of Toronto MississaugaDepartment of Chemical and Physical Sciences, University of Toronto MississaugaLaser Research Centre, Faculty of Physics, Vilnius UniversityLaser Research Centre, Faculty of Physics, Vilnius UniversityLaser Research Centre, Faculty of Physics, Vilnius UniversityLaser Research Centre, Faculty of Physics, Vilnius UniversityLaser Research Centre, Faculty of Physics, Vilnius UniversityAbstract Second harmonic generation (SHG) microscopy is a powerful tool for imaging collagen and other noncentrosymmetric fibrillar structures in biological tissue. Polarimetric SHG measurements provide ultrastructural information about the fibrillar organization in a focal volume (voxel). We present a reduced nonlinear polarimetry method named double Stokes polarimetry (DSP) for quick characterization of chiral $$C_6$$ C 6 symmetry fibers without data fitting that simplifies and speeds up the polarimetric analysis. The method is based on double Stokes-Mueller polarimetry and uses linear and circular incident and outgoing polarization states. The analytical expressions of DSP polarimetric parameters are defined in terms of conventional SHG Stokes vector components. A complex chiral susceptibility (CCS) model is assumed to derive expressions of ultrastructural parameters consisting of the magnitude and phase of molecular complex-valued chiral susceptibility ratio, real-valued achiral ratio, and fiber orientation in a voxel. The ultrastructural parameters are expressed in terms of directly measurable DSP polarimetric parameters. DSP is validated with rat tail tendons sectioned at different orientations. DSP can be applied to investigate the origin of chiral complex-valued susceptibility of collagen, to study modifications of collagen in cancerous tissue, and to map ultrastructural parameters of large areas for whole-slide histopathology.https://doi.org/10.1038/s41598-025-86893-0 |
| spellingShingle | Viktoras Mazeika Kamdin Mirsanaye Leonardo Uribe Castaño Serguei Krouglov Mehdi Alizadeh Mykolas Maciulis Lukas Kontenis Vitalijus Karabanovas Virginijus Barzda Double Stokes polarimetric microscopy for chiral fibrillar aggregates Scientific Reports |
| title | Double Stokes polarimetric microscopy for chiral fibrillar aggregates |
| title_full | Double Stokes polarimetric microscopy for chiral fibrillar aggregates |
| title_fullStr | Double Stokes polarimetric microscopy for chiral fibrillar aggregates |
| title_full_unstemmed | Double Stokes polarimetric microscopy for chiral fibrillar aggregates |
| title_short | Double Stokes polarimetric microscopy for chiral fibrillar aggregates |
| title_sort | double stokes polarimetric microscopy for chiral fibrillar aggregates |
| url | https://doi.org/10.1038/s41598-025-86893-0 |
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