Salmonella enterica Serovars Typhi and Paratyphi A are avirulent in newborn and infant mice even when expressing virulence plasmid genes of Salmonella Typhimurium
Background: Salmonella enterica serovars Typhi and Paratyphi A are human host-restricted pathogens. Therefore, there is no small susceptible animal host that can be used to assess the virulence and safety of vaccine strains derived from these Salmonella serovars. However, infant mice have been use...
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The Journal of Infection in Developing Countries
2010-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/1218 |
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| author | Javier Santander Roy Curtiss III |
| author_facet | Javier Santander Roy Curtiss III |
| author_sort | Javier Santander |
| collection | DOAJ |
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Background: Salmonella enterica serovars Typhi and Paratyphi A are human host-restricted pathogens. Therefore, there is no small susceptible animal host that can be used to assess the virulence and safety of vaccine strains derived from these Salmonella serovars. However, infant mice have been used to evaluate virulence and colonization by another human host-restricted pathogen, Vibrio cholerae.
Methodology: The possibility that infant mice host could be adapted for Salmonella led us to investigate the susceptibility of newborn and infant mice to oral infection with S. Typhi and S. Paratyphi A. Salmonella enterica serovar Typhimurium causes enteric fever in adult mice and this system has been used as a model for human typhoid. The pSTV virulence plasmid, not present in S. Typhi and S. Paratyphi A, plays an essential role in S. Typhimurium colonization and systemic infection of mice. We also conjugated pSTV into S. Typhi and S. Paratyphi A serovars and evaluated these transconjugants in newborn and infant mice.
Results: We determined that the spv virulence genes from the S. Typhimurium virulence plasmid are expressed in S. Typhi and S. Paratyphi A in a RpoS dependent fashion. Also, we determined that S. Typhi and S. Paratyphi A with and without pSTV transiently colonize newborn and infant mice tissues.
Conclusion: Newborn and infant mice infected with S. Typhi and S. Paratyphi A do not succumb to the infection and that carriage of the S. Typhimurium virulence plasmid, pSTV, did not influence these results.
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| format | Article |
| id | doaj-art-baebc18e06a4480d92dcb39cf9b4783a |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2010-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-baebc18e06a4480d92dcb39cf9b4783a2025-08-20T02:27:09ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802010-10-0141110.3855/jidc.1218Salmonella enterica Serovars Typhi and Paratyphi A are avirulent in newborn and infant mice even when expressing virulence plasmid genes of Salmonella TyphimuriumJavier Santander0Roy Curtiss III1The Biodesign Institute, Centre for Infectious Diseases and Vaccinology. Arizona State University, Tempe, ArizonaThe Biodesign Institute, Centre for Infectious Diseases and Vaccinology. Arizona State University, Tempe, Arizona Background: Salmonella enterica serovars Typhi and Paratyphi A are human host-restricted pathogens. Therefore, there is no small susceptible animal host that can be used to assess the virulence and safety of vaccine strains derived from these Salmonella serovars. However, infant mice have been used to evaluate virulence and colonization by another human host-restricted pathogen, Vibrio cholerae. Methodology: The possibility that infant mice host could be adapted for Salmonella led us to investigate the susceptibility of newborn and infant mice to oral infection with S. Typhi and S. Paratyphi A. Salmonella enterica serovar Typhimurium causes enteric fever in adult mice and this system has been used as a model for human typhoid. The pSTV virulence plasmid, not present in S. Typhi and S. Paratyphi A, plays an essential role in S. Typhimurium colonization and systemic infection of mice. We also conjugated pSTV into S. Typhi and S. Paratyphi A serovars and evaluated these transconjugants in newborn and infant mice. Results: We determined that the spv virulence genes from the S. Typhimurium virulence plasmid are expressed in S. Typhi and S. Paratyphi A in a RpoS dependent fashion. Also, we determined that S. Typhi and S. Paratyphi A with and without pSTV transiently colonize newborn and infant mice tissues. Conclusion: Newborn and infant mice infected with S. Typhi and S. Paratyphi A do not succumb to the infection and that carriage of the S. Typhimurium virulence plasmid, pSTV, did not influence these results. https://jidc.org/index.php/journal/article/view/1218Salmonella TyphiSalmonella Paratyphi Anewborn miceinfant micevirulence plasmid |
| spellingShingle | Javier Santander Roy Curtiss III Salmonella enterica Serovars Typhi and Paratyphi A are avirulent in newborn and infant mice even when expressing virulence plasmid genes of Salmonella Typhimurium Journal of Infection in Developing Countries Salmonella Typhi Salmonella Paratyphi A newborn mice infant mice virulence plasmid |
| title | Salmonella enterica Serovars Typhi and Paratyphi A are avirulent in newborn and infant mice even when expressing virulence plasmid genes of Salmonella Typhimurium |
| title_full | Salmonella enterica Serovars Typhi and Paratyphi A are avirulent in newborn and infant mice even when expressing virulence plasmid genes of Salmonella Typhimurium |
| title_fullStr | Salmonella enterica Serovars Typhi and Paratyphi A are avirulent in newborn and infant mice even when expressing virulence plasmid genes of Salmonella Typhimurium |
| title_full_unstemmed | Salmonella enterica Serovars Typhi and Paratyphi A are avirulent in newborn and infant mice even when expressing virulence plasmid genes of Salmonella Typhimurium |
| title_short | Salmonella enterica Serovars Typhi and Paratyphi A are avirulent in newborn and infant mice even when expressing virulence plasmid genes of Salmonella Typhimurium |
| title_sort | salmonella enterica serovars typhi and paratyphi a are avirulent in newborn and infant mice even when expressing virulence plasmid genes of salmonella typhimurium |
| topic | Salmonella Typhi Salmonella Paratyphi A newborn mice infant mice virulence plasmid |
| url | https://jidc.org/index.php/journal/article/view/1218 |
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