Changes Related to Age in Natural and Acquired Systemic Self-IgG Responses in Malaria
Background. Absence of acquired protective immunity in endemic areas children leads to higher susceptibility to severe malaria. To investigate the involvement of regulatory process related to self-reactivity, we evaluated potent changes in auto-antibody reactivity profiles in children and older subj...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Interdisciplinary Perspectives on Infectious Diseases |
| Online Access: | http://dx.doi.org/10.1155/2011/462767 |
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| author | Romuald Dassé Didier Lefranc Sylvain Dubucquoi Patricia Dussart Virginie Dutoit-Lefevre Boualem Sendid François Sombo Mambo Patrick Vermersch Lionel Prin |
| author_facet | Romuald Dassé Didier Lefranc Sylvain Dubucquoi Patricia Dussart Virginie Dutoit-Lefevre Boualem Sendid François Sombo Mambo Patrick Vermersch Lionel Prin |
| author_sort | Romuald Dassé |
| collection | DOAJ |
| description | Background. Absence of acquired protective immunity in endemic areas children leads to higher susceptibility to severe malaria. To investigate the involvement of regulatory process related to self-reactivity, we evaluated potent changes in auto-antibody reactivity profiles in children and older subjects living in malaria-endemic zones comparatively to none-exposed healthy controls. Methods. Analysis of IgG self-reactive footprints was performed using Western blotting against healthy brain antigens. Plasmas of 102 malaria exposed individuals (MEIs) from endemic zone, with or without cerebral malaria (CM) were compared to plasmas from non-endemic controls (NECs). Using linear discriminant and principal component analysis, immune footprints were compared by counting the number, the presence or absence of reactive bands. We identified the most discriminant bands with respect to age and clinical status. Results. A higher number of bands were recognized by IgG auto-antibodies in MEI than in NEC. Characteristic changes in systemic self-IgG-reactive repertoire were found with antigenic bands that discriminate Plasmodium falciparum infections with or without CM according to age. 8 antigenic bands distributed in MEI compared with NEC were identified while 6 other antigenic bands were distributed within MEI according to the age and clinical status. Such distortion might be due to evolutionary processes leading to pathogenic/protective events. |
| format | Article |
| id | doaj-art-baea9c66da8645d689078c6bf0cd2a4c |
| institution | Kabale University |
| issn | 1687-708X 1687-7098 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Interdisciplinary Perspectives on Infectious Diseases |
| spelling | doaj-art-baea9c66da8645d689078c6bf0cd2a4c2025-02-03T05:52:59ZengWileyInterdisciplinary Perspectives on Infectious Diseases1687-708X1687-70982011-01-01201110.1155/2011/462767462767Changes Related to Age in Natural and Acquired Systemic Self-IgG Responses in MalariaRomuald Dassé0Didier Lefranc1Sylvain Dubucquoi2Patricia Dussart3Virginie Dutoit-Lefevre4Boualem Sendid5François Sombo Mambo6Patrick Vermersch7Lionel Prin8Laboratoire d’Immunologie EA 2686, IMPRT-IFR 114, Faculté de Médecine Pôle Recherche, Université Lille 2, 1 Place de Verdun, 59045 Lille Cedex, FranceLaboratoire d’Immunologie EA 2686, IMPRT-IFR 114, Faculté de Médecine Pôle Recherche, Université Lille 2, 1 Place de Verdun, 59045 Lille Cedex, FranceLaboratoire d’Immunologie EA 2686, IMPRT-IFR 114, Faculté de Médecine Pôle Recherche, Université Lille 2, 1 Place de Verdun, 59045 Lille Cedex, FranceLaboratoire d’Immunologie EA 2686, IMPRT-IFR 114, Faculté de Médecine Pôle Recherche, Université Lille 2, 1 Place de Verdun, 59045 Lille Cedex, FranceLaboratoire d’Immunologie EA 2686, IMPRT-IFR 114, Faculté de Médecine Pôle Recherche, Université Lille 2, 1 Place de Verdun, 59045 Lille Cedex, FranceLaboratoire de Parasitologie et de Mycologie, Institute de Biologie et Pathologie, CHRU de Lille 59037 Lille, FranceLaboratoire d’Immunologie et Hématologie du CHU-Cocody, Abidjan, Cote D’IvoireService de Neurologie D, Hôpital Roger Salengro, 59037 Lille Cedex, FranceLaboratoire d’Immunologie EA 2686, IMPRT-IFR 114, Faculté de Médecine Pôle Recherche, Université Lille 2, 1 Place de Verdun, 59045 Lille Cedex, FranceBackground. Absence of acquired protective immunity in endemic areas children leads to higher susceptibility to severe malaria. To investigate the involvement of regulatory process related to self-reactivity, we evaluated potent changes in auto-antibody reactivity profiles in children and older subjects living in malaria-endemic zones comparatively to none-exposed healthy controls. Methods. Analysis of IgG self-reactive footprints was performed using Western blotting against healthy brain antigens. Plasmas of 102 malaria exposed individuals (MEIs) from endemic zone, with or without cerebral malaria (CM) were compared to plasmas from non-endemic controls (NECs). Using linear discriminant and principal component analysis, immune footprints were compared by counting the number, the presence or absence of reactive bands. We identified the most discriminant bands with respect to age and clinical status. Results. A higher number of bands were recognized by IgG auto-antibodies in MEI than in NEC. Characteristic changes in systemic self-IgG-reactive repertoire were found with antigenic bands that discriminate Plasmodium falciparum infections with or without CM according to age. 8 antigenic bands distributed in MEI compared with NEC were identified while 6 other antigenic bands were distributed within MEI according to the age and clinical status. Such distortion might be due to evolutionary processes leading to pathogenic/protective events.http://dx.doi.org/10.1155/2011/462767 |
| spellingShingle | Romuald Dassé Didier Lefranc Sylvain Dubucquoi Patricia Dussart Virginie Dutoit-Lefevre Boualem Sendid François Sombo Mambo Patrick Vermersch Lionel Prin Changes Related to Age in Natural and Acquired Systemic Self-IgG Responses in Malaria Interdisciplinary Perspectives on Infectious Diseases |
| title | Changes Related to Age in Natural and Acquired Systemic Self-IgG Responses in Malaria |
| title_full | Changes Related to Age in Natural and Acquired Systemic Self-IgG Responses in Malaria |
| title_fullStr | Changes Related to Age in Natural and Acquired Systemic Self-IgG Responses in Malaria |
| title_full_unstemmed | Changes Related to Age in Natural and Acquired Systemic Self-IgG Responses in Malaria |
| title_short | Changes Related to Age in Natural and Acquired Systemic Self-IgG Responses in Malaria |
| title_sort | changes related to age in natural and acquired systemic self igg responses in malaria |
| url | http://dx.doi.org/10.1155/2011/462767 |
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