Enraizamiento in vitro y la influencia de cuatro tipos de sustrato en la aclimatación de Queñoa Polylepis rugulosa Bitter

Queñoais a forest species of great importance and its propagation in a conventional way is a limitation when it is desired to produce it on a large scale, for which the micropropagation technique was used, with the objective of evaluating the effect of the combination of auxin and c...

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Main Authors: Carmen Rosa Mollohuanca Ccuno, Edwin Bustamante Salas, Luis Fernando Mayta Anco, ROXANA MARGARITA BARDALES ALVAREZ
Format: Article
Language:English
Published: Universidad Nacional de Tumbes 2022-03-01
Series:Manglar
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Online Access:https://revistas.untumbes.edu.pe/index.php/manglar/article/view/297
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author Carmen Rosa Mollohuanca Ccuno
Edwin Bustamante Salas
Luis Fernando Mayta Anco
ROXANA MARGARITA BARDALES ALVAREZ
author_facet Carmen Rosa Mollohuanca Ccuno
Edwin Bustamante Salas
Luis Fernando Mayta Anco
ROXANA MARGARITA BARDALES ALVAREZ
author_sort Carmen Rosa Mollohuanca Ccuno
collection DOAJ
description Queñoais a forest species of great importance and its propagation in a conventional way is a limitation when it is desired to produce it on a large scale, for which the micropropagation technique was used, with the objective of evaluating the effect of the combination of auxin and cytokinin in the rooting of Polylepis rugulosaBitter buds, establishing 12 treatments with different concentrations of IBA and BAP, likewise during acclimatization, the influence of four types of substrates based on ground, peat and cow manure in different proportions was tested, where seedlings weretransplanted rooted, which were subjected to greenhouse conditions. The best response in the rooting percentage was 98%, this result was obtained with the concentration of 0.5 μM AIB + 0.05 μM BAP, which presented 1.9 roots and an average root length of 7mm. A survival percentage of 87.5% was reached during acclimatization; cow manure-basedtreatments showed higher values regarding the variable’sseedling height, number of leaves and root length. Therefore, the result will make it possible to mass produce plants and initiate conservation and recovery programs.
format Article
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institution Kabale University
issn 1816-7667
2414-1046
language English
publishDate 2022-03-01
publisher Universidad Nacional de Tumbes
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series Manglar
spelling doaj-art-badef7e35cf844cb96607454d397e4632025-01-03T01:14:57ZengUniversidad Nacional de TumbesManglar1816-76672414-10462022-03-0119110.17268/manglar.2022.003Enraizamiento in vitro y la influencia de cuatro tipos de sustrato en la aclimatación de Queñoa Polylepis rugulosa BitterCarmen Rosa Mollohuanca Ccuno0https://orcid.org/0000-0002-9105-8501Edwin Bustamante Salas1https://orcid.org/0000-0002-3958-7860Luis Fernando Mayta Anco2https://orcid.org/0000-0001-8359-4876ROXANA MARGARITA BARDALES ALVAREZ3https://orcid.org/0000-0002-5137-4067Laboratorio de Biotecnología Vegetal, Sociedad Minera Cerro Verde, Av. de Las Tradiciones S/N, Congata, Uchumayo, Arequipa, PerúLaboratorio de Biotecnología Vegetal, Sociedad Minera Cerro Verde, Av. de Las Tradiciones S/N, Congata, Uchumayo, Arequipa, PerúLaboratorio de Biotecnología Vegetal, Sociedad Minera Cerro Verde, Av. de Las Tradiciones S/N, Congata, Uchumayo, Arequipa, PerúFacultad de Agronomía, Universidad Nacional de San Agustín de Arequipa, Urb. Aurora S/N, Arequipa, PerúQueñoais a forest species of great importance and its propagation in a conventional way is a limitation when it is desired to produce it on a large scale, for which the micropropagation technique was used, with the objective of evaluating the effect of the combination of auxin and cytokinin in the rooting of Polylepis rugulosaBitter buds, establishing 12 treatments with different concentrations of IBA and BAP, likewise during acclimatization, the influence of four types of substrates based on ground, peat and cow manure in different proportions was tested, where seedlings weretransplanted rooted, which were subjected to greenhouse conditions. The best response in the rooting percentage was 98%, this result was obtained with the concentration of 0.5 μM AIB + 0.05 μM BAP, which presented 1.9 roots and an average root length of 7mm. A survival percentage of 87.5% was reached during acclimatization; cow manure-basedtreatments showed higher values regarding the variable’sseedling height, number of leaves and root length. Therefore, the result will make it possible to mass produce plants and initiate conservation and recovery programs.https://revistas.untumbes.edu.pe/index.php/manglar/article/view/297polypepis rugulosabitter; indole-3-butyric acid; 6-benzylaminopurine; in vitro rooting; acclimatization.
spellingShingle Carmen Rosa Mollohuanca Ccuno
Edwin Bustamante Salas
Luis Fernando Mayta Anco
ROXANA MARGARITA BARDALES ALVAREZ
Enraizamiento in vitro y la influencia de cuatro tipos de sustrato en la aclimatación de Queñoa Polylepis rugulosa Bitter
Manglar
polypepis rugulosabitter; indole-3-butyric acid; 6-benzylaminopurine; in vitro rooting; acclimatization.
title Enraizamiento in vitro y la influencia de cuatro tipos de sustrato en la aclimatación de Queñoa Polylepis rugulosa Bitter
title_full Enraizamiento in vitro y la influencia de cuatro tipos de sustrato en la aclimatación de Queñoa Polylepis rugulosa Bitter
title_fullStr Enraizamiento in vitro y la influencia de cuatro tipos de sustrato en la aclimatación de Queñoa Polylepis rugulosa Bitter
title_full_unstemmed Enraizamiento in vitro y la influencia de cuatro tipos de sustrato en la aclimatación de Queñoa Polylepis rugulosa Bitter
title_short Enraizamiento in vitro y la influencia de cuatro tipos de sustrato en la aclimatación de Queñoa Polylepis rugulosa Bitter
title_sort enraizamiento in vitro y la influencia de cuatro tipos de sustrato en la aclimatacion de quenoa polylepis rugulosa bitter
topic polypepis rugulosabitter; indole-3-butyric acid; 6-benzylaminopurine; in vitro rooting; acclimatization.
url https://revistas.untumbes.edu.pe/index.php/manglar/article/view/297
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