Effects of Short-Term Feeding with Diets Containing Insect Meal on the Gut Microbiota of African Catfish Hybrids
The impact of short-term feeding of three distinct diets containing insect meals on the intestinal microbiota of African catfish hybrid (<i>Clarias gariepinus × Heterobranchus longifilis</i>) juveniles was examined. The animals received experimental diets containing 30% insect meals deri...
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MDPI AG
2025-05-01
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| author | Balázs Libisch Zsuzsanna J. Sándor Tibor Keresztény Chioma Lilian Ozoaduche Péter P. Papp Katalin Posta Janka Biró Viktor Stojkov Vojislav Banjac Nóra Adányi Mária Berki Éva Lengyel-Kónya Rita Tömösközi-Farkas Ferenc Olasz |
| author_facet | Balázs Libisch Zsuzsanna J. Sándor Tibor Keresztény Chioma Lilian Ozoaduche Péter P. Papp Katalin Posta Janka Biró Viktor Stojkov Vojislav Banjac Nóra Adányi Mária Berki Éva Lengyel-Kónya Rita Tömösközi-Farkas Ferenc Olasz |
| author_sort | Balázs Libisch |
| collection | DOAJ |
| description | The impact of short-term feeding of three distinct diets containing insect meals on the intestinal microbiota of African catfish hybrid (<i>Clarias gariepinus × Heterobranchus longifilis</i>) juveniles was examined. The animals received experimental diets containing 30% insect meals derived from black soldier-fly larvae (BSL) (<i>Hermetia illucens</i>), yellow mealworm (<i>Tenebrio molitor</i>) or blue bottle-fly larvae (<i>Calliphora vicina</i>) for 18 days. The relative abundance of the <i>Bacillaceae</i>, the <i>Planococcaceae</i> and other bacteria significantly increased (<i>p</i> < 0.05) in the intestinal microbiota of the BSL group and also in the pooled group of the three catfish groups that received insect meals. Several strains of the <i>Bacillales</i> cultured from BSL feed had higher (<i>p</i> < 0.05) abundance in the intestinal microbiota of the BSL group compared to those of the control group. Among these <i>Bacillales</i> strains, a single <i>fosB</i> antibiotic resistance gene was identified. In the gut resistomes of both the BSL and the control catfish groups, the <i>tetA(P)</i>, <i>tetB(P)</i> and <i>lnu(C)</i> antibiotic-resistance determinants were detected, while <i>fosB</i> was detected only in the BSL group. Overall, the study showed that a short-term shift to diets containing insect meals can induce significant (q < 0.05) changes in the gut microbiota of the African catfish without the development of reduced α-diversity and without the overgrowth of bacteria pathogenic to fish. |
| format | Article |
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| institution | OA Journals |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-05-01 |
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| series | Animals |
| spelling | doaj-art-bade44bb4d0f4da594ead8cd16a4a3712025-08-20T02:24:43ZengMDPI AGAnimals2076-26152025-05-01159133810.3390/ani15091338Effects of Short-Term Feeding with Diets Containing Insect Meal on the Gut Microbiota of African Catfish HybridsBalázs Libisch0Zsuzsanna J. Sándor1Tibor Keresztény2Chioma Lilian Ozoaduche3Péter P. Papp4Katalin Posta5Janka Biró6Viktor Stojkov7Vojislav Banjac8Nóra Adányi9Mária Berki10Éva Lengyel-Kónya11Rita Tömösközi-Farkas12Ferenc Olasz13Agribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, HungaryResearch Center for Fisheries and Aquaculture, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, 5540 Szarvas, HungaryAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, HungaryAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, HungaryAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, HungaryAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, HungaryResearch Center for Fisheries and Aquaculture, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, 5540 Szarvas, HungaryInstitute of Food Technology, University of Novi Sad, 21000 Novi Sad, SerbiaInstitute of Food Technology, University of Novi Sad, 21000 Novi Sad, SerbiaInstitute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, 1118 Budapest, HungaryInstitute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, 1118 Budapest, HungaryInstitute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, 1118 Budapest, HungaryInstitute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, 1118 Budapest, HungaryAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, HungaryThe impact of short-term feeding of three distinct diets containing insect meals on the intestinal microbiota of African catfish hybrid (<i>Clarias gariepinus × Heterobranchus longifilis</i>) juveniles was examined. The animals received experimental diets containing 30% insect meals derived from black soldier-fly larvae (BSL) (<i>Hermetia illucens</i>), yellow mealworm (<i>Tenebrio molitor</i>) or blue bottle-fly larvae (<i>Calliphora vicina</i>) for 18 days. The relative abundance of the <i>Bacillaceae</i>, the <i>Planococcaceae</i> and other bacteria significantly increased (<i>p</i> < 0.05) in the intestinal microbiota of the BSL group and also in the pooled group of the three catfish groups that received insect meals. Several strains of the <i>Bacillales</i> cultured from BSL feed had higher (<i>p</i> < 0.05) abundance in the intestinal microbiota of the BSL group compared to those of the control group. Among these <i>Bacillales</i> strains, a single <i>fosB</i> antibiotic resistance gene was identified. In the gut resistomes of both the BSL and the control catfish groups, the <i>tetA(P)</i>, <i>tetB(P)</i> and <i>lnu(C)</i> antibiotic-resistance determinants were detected, while <i>fosB</i> was detected only in the BSL group. Overall, the study showed that a short-term shift to diets containing insect meals can induce significant (q < 0.05) changes in the gut microbiota of the African catfish without the development of reduced α-diversity and without the overgrowth of bacteria pathogenic to fish.https://www.mdpi.com/2076-2615/15/9/1338African catfishinsect mealintestinal microbiota<i>Bacillus</i>chitinaseantibiotic resistance |
| spellingShingle | Balázs Libisch Zsuzsanna J. Sándor Tibor Keresztény Chioma Lilian Ozoaduche Péter P. Papp Katalin Posta Janka Biró Viktor Stojkov Vojislav Banjac Nóra Adányi Mária Berki Éva Lengyel-Kónya Rita Tömösközi-Farkas Ferenc Olasz Effects of Short-Term Feeding with Diets Containing Insect Meal on the Gut Microbiota of African Catfish Hybrids Animals African catfish insect meal intestinal microbiota <i>Bacillus</i> chitinase antibiotic resistance |
| title | Effects of Short-Term Feeding with Diets Containing Insect Meal on the Gut Microbiota of African Catfish Hybrids |
| title_full | Effects of Short-Term Feeding with Diets Containing Insect Meal on the Gut Microbiota of African Catfish Hybrids |
| title_fullStr | Effects of Short-Term Feeding with Diets Containing Insect Meal on the Gut Microbiota of African Catfish Hybrids |
| title_full_unstemmed | Effects of Short-Term Feeding with Diets Containing Insect Meal on the Gut Microbiota of African Catfish Hybrids |
| title_short | Effects of Short-Term Feeding with Diets Containing Insect Meal on the Gut Microbiota of African Catfish Hybrids |
| title_sort | effects of short term feeding with diets containing insect meal on the gut microbiota of african catfish hybrids |
| topic | African catfish insect meal intestinal microbiota <i>Bacillus</i> chitinase antibiotic resistance |
| url | https://www.mdpi.com/2076-2615/15/9/1338 |
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