Biotechnological Micronutrient Production: Recombinant DNA Technology-Based Vitamin A Synthesis
Vitamin A is an essential micronutrient and has important functions such as vision, growth, reproduction embryogenesis, cellular differentiation, and proliferation, immune function and epithelial protector in the organism. Biotechnological production of vitamins is increasing due to their advantages...
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| Format: | Article |
| Language: | English |
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Hasan Eleroğlu
2022-08-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/5069 |
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| author | Asli Giray Sibel Pekdemir |
| author_facet | Asli Giray Sibel Pekdemir |
| author_sort | Asli Giray |
| collection | DOAJ |
| description | Vitamin A is an essential micronutrient and has important functions such as vision, growth, reproduction embryogenesis, cellular differentiation, and proliferation, immune function and epithelial protector in the organism. Biotechnological production of vitamins is increasing due to their advantages and significant advances. The vitreoscilla hemoglobin (VHb) gene is extremely effective in binding oxygen and conducting it under hypoxic conditions. In this study, the production of vitamin A in E. herbicola (wild type) and its recombinant strains was investigated in LB medium and M9 medium (containing high concentrations (1%) of different carbon sources). The maximum production of vitamin A of the recombinant strain with the hemoglobin gene (vgb+) was observed in including glucose and sucrose M9 medium and their total product levels in vgb+ recombinant strain were 0.14 µg/ml and 0.1 µg/ml, respectively. The vitamin A production in the M9 medium with glucose and sucrose were 2-fold and 1.4- fold higher than that of the wild strain, respectively. The extracellular product level (0.07 µg/ml) in LB was 7-fold higher than wild strain at 48 h. These results reveal that the expression of VHb in E. herbicola in the both LB and M9 medium (containing 1% glucose and 1% sucrose, specially) increase the vitamin A production. |
| format | Article |
| id | doaj-art-bad818dfa4e2472380ce9adbf26606a5 |
| institution | OA Journals |
| issn | 2148-127X |
| language | English |
| publishDate | 2022-08-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-bad818dfa4e2472380ce9adbf26606a52025-08-20T02:19:21ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2022-08-011081420142510.24925/turjaf.v10i8.1420-1425.50692554Biotechnological Micronutrient Production: Recombinant DNA Technology-Based Vitamin A SynthesisAsli Giray0Sibel Pekdemir1Department of Genetics and Bioengineering, Faculty of Engineering, Alanya Alaaddin Keykubat University, 07425 AntalyaDepartment of Chemistry, Bingöl University, 12000 BingölVitamin A is an essential micronutrient and has important functions such as vision, growth, reproduction embryogenesis, cellular differentiation, and proliferation, immune function and epithelial protector in the organism. Biotechnological production of vitamins is increasing due to their advantages and significant advances. The vitreoscilla hemoglobin (VHb) gene is extremely effective in binding oxygen and conducting it under hypoxic conditions. In this study, the production of vitamin A in E. herbicola (wild type) and its recombinant strains was investigated in LB medium and M9 medium (containing high concentrations (1%) of different carbon sources). The maximum production of vitamin A of the recombinant strain with the hemoglobin gene (vgb+) was observed in including glucose and sucrose M9 medium and their total product levels in vgb+ recombinant strain were 0.14 µg/ml and 0.1 µg/ml, respectively. The vitamin A production in the M9 medium with glucose and sucrose were 2-fold and 1.4- fold higher than that of the wild strain, respectively. The extracellular product level (0.07 µg/ml) in LB was 7-fold higher than wild strain at 48 h. These results reveal that the expression of VHb in E. herbicola in the both LB and M9 medium (containing 1% glucose and 1% sucrose, specially) increase the vitamin A production.http://www.agrifoodscience.com/index.php/TURJAF/article/view/5069biotechnological productionvitamin avitreoscilla hemoglobin (vhb)erwinia herbicolamicronutrient |
| spellingShingle | Asli Giray Sibel Pekdemir Biotechnological Micronutrient Production: Recombinant DNA Technology-Based Vitamin A Synthesis Turkish Journal of Agriculture: Food Science and Technology biotechnological production vitamin a vitreoscilla hemoglobin (vhb) erwinia herbicola micronutrient |
| title | Biotechnological Micronutrient Production: Recombinant DNA Technology-Based Vitamin A Synthesis |
| title_full | Biotechnological Micronutrient Production: Recombinant DNA Technology-Based Vitamin A Synthesis |
| title_fullStr | Biotechnological Micronutrient Production: Recombinant DNA Technology-Based Vitamin A Synthesis |
| title_full_unstemmed | Biotechnological Micronutrient Production: Recombinant DNA Technology-Based Vitamin A Synthesis |
| title_short | Biotechnological Micronutrient Production: Recombinant DNA Technology-Based Vitamin A Synthesis |
| title_sort | biotechnological micronutrient production recombinant dna technology based vitamin a synthesis |
| topic | biotechnological production vitamin a vitreoscilla hemoglobin (vhb) erwinia herbicola micronutrient |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/5069 |
| work_keys_str_mv | AT asligiray biotechnologicalmicronutrientproductionrecombinantdnatechnologybasedvitaminasynthesis AT sibelpekdemir biotechnologicalmicronutrientproductionrecombinantdnatechnologybasedvitaminasynthesis |