Screening of antagonistic bacteria against fungal phytopathogens

Water samples, bottom sediments and biofilms of lake were investigated to the microbial diversity. One hundred twenty-two isolated microorganisms were identified by cultural, morphological and biochemical characteristics. Bacterial strains were screened to evaluate their ability to produce amylase,...

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Main Authors: Nina BOGDAN, Valerina SLANINA, Tamara SÎRBU, Ludmila BALAN, Robert Marian RUGINESCU
Format: Article
Language:English
Published: Galati University Press 2025-03-01
Series:ACROSS
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Online Access:https://www.gup.ugal.ro/ugaljournals/index.php/across/article/view/7462
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author Nina BOGDAN
Valerina SLANINA
Tamara SÎRBU
Ludmila BALAN
Robert Marian RUGINESCU
author_facet Nina BOGDAN
Valerina SLANINA
Tamara SÎRBU
Ludmila BALAN
Robert Marian RUGINESCU
author_sort Nina BOGDAN
collection DOAJ
description Water samples, bottom sediments and biofilms of lake were investigated to the microbial diversity. One hundred twenty-two isolated microorganisms were identified by cultural, morphological and biochemical characteristics. Bacterial strains were screened to evaluate their ability to produce amylase, catalase, lipase and cellulase enzymes. Aquatic bacterial strains are active producers of valuable exoenzymes with antimicrobial properties. Isolates demonstrated developed enzymatic complex and antifungal activity against phytopathogens - Alternaria alternata, Botrytis cinerea, Fusarium solani, Fusarium oxysporum. Most active and stable to enzymatic and antifungal activities strains were identified as: Bacillus velezensis (Bacillus amyloliquefaciens) and Micrococcus yunnanensis (Micrococcus luteus) by PCR-based molecular analysis. Due to results of antagonistic activity in this study aquatic bacteria are promising for application in agriculture sector.
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spelling doaj-art-bad8151dee824491bb0fa809d6bf232f2025-08-20T02:16:06ZengGalati University PressACROSS2602-14632025-03-0162Screening of antagonistic bacteria against fungal phytopathogensNina BOGDAN0Valerina SLANINA1Tamara SÎRBU2Ludmila BALAN3Robert Marian RUGINESCU4Institute of Microbiology and Biotechnology (Moldova)Institute of Microbiology and Biotechnology (Moldova)Institute of Microbiology and Biotechnology (Moldova)Institute of Microbiology and Biotechnology (Moldova)Institute of Biology Bucharest, Romanian Academy (Romania) Water samples, bottom sediments and biofilms of lake were investigated to the microbial diversity. One hundred twenty-two isolated microorganisms were identified by cultural, morphological and biochemical characteristics. Bacterial strains were screened to evaluate their ability to produce amylase, catalase, lipase and cellulase enzymes. Aquatic bacterial strains are active producers of valuable exoenzymes with antimicrobial properties. Isolates demonstrated developed enzymatic complex and antifungal activity against phytopathogens - Alternaria alternata, Botrytis cinerea, Fusarium solani, Fusarium oxysporum. Most active and stable to enzymatic and antifungal activities strains were identified as: Bacillus velezensis (Bacillus amyloliquefaciens) and Micrococcus yunnanensis (Micrococcus luteus) by PCR-based molecular analysis. Due to results of antagonistic activity in this study aquatic bacteria are promising for application in agriculture sector. https://www.gup.ugal.ro/ugaljournals/index.php/across/article/view/7462bacteriaphytopathogensenzyme
spellingShingle Nina BOGDAN
Valerina SLANINA
Tamara SÎRBU
Ludmila BALAN
Robert Marian RUGINESCU
Screening of antagonistic bacteria against fungal phytopathogens
ACROSS
bacteria
phytopathogens
enzyme
title Screening of antagonistic bacteria against fungal phytopathogens
title_full Screening of antagonistic bacteria against fungal phytopathogens
title_fullStr Screening of antagonistic bacteria against fungal phytopathogens
title_full_unstemmed Screening of antagonistic bacteria against fungal phytopathogens
title_short Screening of antagonistic bacteria against fungal phytopathogens
title_sort screening of antagonistic bacteria against fungal phytopathogens
topic bacteria
phytopathogens
enzyme
url https://www.gup.ugal.ro/ugaljournals/index.php/across/article/view/7462
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AT ludmilabalan screeningofantagonisticbacteriaagainstfungalphytopathogens
AT robertmarianruginescu screeningofantagonisticbacteriaagainstfungalphytopathogens