Utilization of surfactant assisted zinc oxide nanodisk against breast cancer cells
The current work displayed the efficacy of the nanodisk shaped zinc oxide nanostructures (NDs-ZnONSs) processed via hydrothermal solution process and studies the cytotoxicity against human breast cancer cells (MCF-7). The NDs-ZnONSs were analyzed by using instruments such as X-ray diffraction patter...
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Experimental Nanoscience |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/17458080.2023.2228494 |
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| author | Abdulsalam Alkhudhayri |
| author_facet | Abdulsalam Alkhudhayri |
| author_sort | Abdulsalam Alkhudhayri |
| collection | DOAJ |
| description | The current work displayed the efficacy of the nanodisk shaped zinc oxide nanostructures (NDs-ZnONSs) processed via hydrothermal solution process and studies the cytotoxicity against human breast cancer cells (MCF-7). The NDs-ZnONSs were analyzed by using instruments such as X-ray diffraction pattern (XRD), Field emission scanning electron microscopy (FESEM), and Fourier transform infra-red spectroscopy (FTIR). The XRD and FESEM shows the crystallinity and morphology of NDs-ZnONSs states the estimated width size of each sheet diameter and length are ∼20-25 nm and 2-3 µm respectively. The cytotoxicity of MCF-7 cells with NDs-ZnONSs at different concentrations (1, 2, 5, 10, 25, 50 and 100 µg/mL) were examined via MTT and NRU assays. The results showed that at initial (1, 2 and 5 µg/ml) concentrations of NDs-ZnONSs didn’t show any cytotoxicity in cancer cells but higher concentrations (25, 50 and 100 µg/mL) of NDs-ZnONSs displayed a remarkable inhibition in cancer cells growth and morphology. Further, the reactive oxygen species (ROS) generation was also examined with NDs-ZnONSs at different concentrations and result showed that it increased with increasing the concentration of NDs-ZnONSs in MCF-7 cells. The expression of apoptotic genes (p53, bax, casp-3) including control via qRT-PCR, reveals that mRNA level of apoptotic genes with NDs-ZnONSs were upregulated. |
| format | Article |
| id | doaj-art-bad559db26cc433886b7d43ce4722488 |
| institution | Kabale University |
| issn | 1745-8080 1745-8099 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
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| series | Journal of Experimental Nanoscience |
| spelling | doaj-art-bad559db26cc433886b7d43ce47224882024-11-25T10:28:58ZengTaylor & Francis GroupJournal of Experimental Nanoscience1745-80801745-80992023-12-0118110.1080/17458080.2023.2228494Utilization of surfactant assisted zinc oxide nanodisk against breast cancer cellsAbdulsalam Alkhudhayri0Department of Biology, College of Science, University of Hafr Al Batin, Hafr Al Batin, Saudi ArabiaThe current work displayed the efficacy of the nanodisk shaped zinc oxide nanostructures (NDs-ZnONSs) processed via hydrothermal solution process and studies the cytotoxicity against human breast cancer cells (MCF-7). The NDs-ZnONSs were analyzed by using instruments such as X-ray diffraction pattern (XRD), Field emission scanning electron microscopy (FESEM), and Fourier transform infra-red spectroscopy (FTIR). The XRD and FESEM shows the crystallinity and morphology of NDs-ZnONSs states the estimated width size of each sheet diameter and length are ∼20-25 nm and 2-3 µm respectively. The cytotoxicity of MCF-7 cells with NDs-ZnONSs at different concentrations (1, 2, 5, 10, 25, 50 and 100 µg/mL) were examined via MTT and NRU assays. The results showed that at initial (1, 2 and 5 µg/ml) concentrations of NDs-ZnONSs didn’t show any cytotoxicity in cancer cells but higher concentrations (25, 50 and 100 µg/mL) of NDs-ZnONSs displayed a remarkable inhibition in cancer cells growth and morphology. Further, the reactive oxygen species (ROS) generation was also examined with NDs-ZnONSs at different concentrations and result showed that it increased with increasing the concentration of NDs-ZnONSs in MCF-7 cells. The expression of apoptotic genes (p53, bax, casp-3) including control via qRT-PCR, reveals that mRNA level of apoptotic genes with NDs-ZnONSs were upregulated.https://www.tandfonline.com/doi/10.1080/17458080.2023.2228494Nano diskzinc oxide nanostructuresX-ray diffraction patternFESEM and FTIR |
| spellingShingle | Abdulsalam Alkhudhayri Utilization of surfactant assisted zinc oxide nanodisk against breast cancer cells Journal of Experimental Nanoscience Nano disk zinc oxide nanostructures X-ray diffraction pattern FESEM and FTIR |
| title | Utilization of surfactant assisted zinc oxide nanodisk against breast cancer cells |
| title_full | Utilization of surfactant assisted zinc oxide nanodisk against breast cancer cells |
| title_fullStr | Utilization of surfactant assisted zinc oxide nanodisk against breast cancer cells |
| title_full_unstemmed | Utilization of surfactant assisted zinc oxide nanodisk against breast cancer cells |
| title_short | Utilization of surfactant assisted zinc oxide nanodisk against breast cancer cells |
| title_sort | utilization of surfactant assisted zinc oxide nanodisk against breast cancer cells |
| topic | Nano disk zinc oxide nanostructures X-ray diffraction pattern FESEM and FTIR |
| url | https://www.tandfonline.com/doi/10.1080/17458080.2023.2228494 |
| work_keys_str_mv | AT abdulsalamalkhudhayri utilizationofsurfactantassistedzincoxidenanodiskagainstbreastcancercells |