Prevalence of <i>Vibrio</i> spp. in Seafood from German Supermarkets and Fish Markets

This study investigates the prevalence of <i>Vibrio</i> spp. in seafood from supermarkets and fish markets in Berlin, Germany. A total of 306 seafood samples, including shrimp and mussels, were bought from supermarkets between March 2023 and January 2024. Samples were analysed using the...

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Main Authors: Christopher Zeidler, Vanessa Szott, Thomas Alter, Stephan Huehn-Lindenbein, Susanne Fleischmann
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Foods
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Online Access:https://www.mdpi.com/2304-8158/13/24/3987
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author Christopher Zeidler
Vanessa Szott
Thomas Alter
Stephan Huehn-Lindenbein
Susanne Fleischmann
author_facet Christopher Zeidler
Vanessa Szott
Thomas Alter
Stephan Huehn-Lindenbein
Susanne Fleischmann
author_sort Christopher Zeidler
collection DOAJ
description This study investigates the prevalence of <i>Vibrio</i> spp. in seafood from supermarkets and fish markets in Berlin, Germany. A total of 306 seafood samples, including shrimp and mussels, were bought from supermarkets between March 2023 and January 2024. Samples were analysed using the ISO standard method and multiplex PCR to identify <i>V. parahaemolyticus</i>, <i>V. alginolyticus, V. cholerae</i> and <i>V. vulnificus</i>. The results indicated an overall <i>Vibrio</i> spp. prevalence of 56%. Among the positive samples, the most prevalent species found was <i>V. parahaemolyticus</i> (58%), followed by <i>V. alginolyticus</i> (42%), <i>V. cholerae</i> non-O1/non-O139 (25%), and <i>V. vulnificus</i> (4%). Samples obtained from supermarkets exhibited a lower prevalence (50%) than those received from fish markets (91%). Virulence genes such as <i>ctxA</i>, <i>tdh</i>, or <i>trh</i> were not detected in the respective <i>Vibrio</i> species. Nevertheless, the high prevalence underscores the need and urgency of continuous seafood surveillance.
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spelling doaj-art-bad23d8edada47c5848d20e3d9e52bb22025-08-20T02:00:46ZengMDPI AGFoods2304-81582024-12-011324398710.3390/foods13243987Prevalence of <i>Vibrio</i> spp. in Seafood from German Supermarkets and Fish MarketsChristopher Zeidler0Vanessa Szott1Thomas Alter2Stephan Huehn-Lindenbein3Susanne Fleischmann4School of Veterinary Medicine Center for Veterinary Public Health, Institute of Food Safety and Hygiene, Freie Universität Berlin, Königsweg 69, 14163 Berlin, GermanySchool of Veterinary Medicine Center for Veterinary Public Health, Institute of Food Safety and Hygiene, Freie Universität Berlin, Königsweg 69, 14163 Berlin, GermanySchool of Veterinary Medicine Center for Veterinary Public Health, Institute of Food Safety and Hygiene, Freie Universität Berlin, Königsweg 69, 14163 Berlin, GermanyFaculty of Life Sciences and Technology, University of Applied Sciences, Berliner Hochschule für Technik, Luxemburger Str. 10, 13353 Berlin, GermanySchool of Veterinary Medicine Center for Veterinary Public Health, Institute of Food Safety and Hygiene, Freie Universität Berlin, Königsweg 69, 14163 Berlin, GermanyThis study investigates the prevalence of <i>Vibrio</i> spp. in seafood from supermarkets and fish markets in Berlin, Germany. A total of 306 seafood samples, including shrimp and mussels, were bought from supermarkets between March 2023 and January 2024. Samples were analysed using the ISO standard method and multiplex PCR to identify <i>V. parahaemolyticus</i>, <i>V. alginolyticus, V. cholerae</i> and <i>V. vulnificus</i>. The results indicated an overall <i>Vibrio</i> spp. prevalence of 56%. Among the positive samples, the most prevalent species found was <i>V. parahaemolyticus</i> (58%), followed by <i>V. alginolyticus</i> (42%), <i>V. cholerae</i> non-O1/non-O139 (25%), and <i>V. vulnificus</i> (4%). Samples obtained from supermarkets exhibited a lower prevalence (50%) than those received from fish markets (91%). Virulence genes such as <i>ctxA</i>, <i>tdh</i>, or <i>trh</i> were not detected in the respective <i>Vibrio</i> species. Nevertheless, the high prevalence underscores the need and urgency of continuous seafood surveillance.https://www.mdpi.com/2304-8158/13/24/3987human pathogen <i>Vibrio</i> spp.prevalenceseafoodfood safetysupermarketsfish markets
spellingShingle Christopher Zeidler
Vanessa Szott
Thomas Alter
Stephan Huehn-Lindenbein
Susanne Fleischmann
Prevalence of <i>Vibrio</i> spp. in Seafood from German Supermarkets and Fish Markets
Foods
human pathogen <i>Vibrio</i> spp.
prevalence
seafood
food safety
supermarkets
fish markets
title Prevalence of <i>Vibrio</i> spp. in Seafood from German Supermarkets and Fish Markets
title_full Prevalence of <i>Vibrio</i> spp. in Seafood from German Supermarkets and Fish Markets
title_fullStr Prevalence of <i>Vibrio</i> spp. in Seafood from German Supermarkets and Fish Markets
title_full_unstemmed Prevalence of <i>Vibrio</i> spp. in Seafood from German Supermarkets and Fish Markets
title_short Prevalence of <i>Vibrio</i> spp. in Seafood from German Supermarkets and Fish Markets
title_sort prevalence of i vibrio i spp in seafood from german supermarkets and fish markets
topic human pathogen <i>Vibrio</i> spp.
prevalence
seafood
food safety
supermarkets
fish markets
url https://www.mdpi.com/2304-8158/13/24/3987
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AT vanessaszott prevalenceofivibrioisppinseafoodfromgermansupermarketsandfishmarkets
AT thomasalter prevalenceofivibrioisppinseafoodfromgermansupermarketsandfishmarkets
AT stephanhuehnlindenbein prevalenceofivibrioisppinseafoodfromgermansupermarketsandfishmarkets
AT susannefleischmann prevalenceofivibrioisppinseafoodfromgermansupermarketsandfishmarkets