Expression and Potential Role of MMP-9 in Intrauterine Adhesion
Objective. Intrauterine adhesions affect menstruation and fertility, and endometrial fibrosis is the final manifestation of IUA. MMP-9 is closely related to fibrosis. The purpose of the study was to assess the role of MMP-9 in intrauterine adhesion (IUA) in rats and patients. Methods. 40 rats and 24...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2021/6676510 |
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| author | Congqing Li Wenyan Wang Shiying Sun Youjiang Xu Ziang Fang Lin Cong |
| author_facet | Congqing Li Wenyan Wang Shiying Sun Youjiang Xu Ziang Fang Lin Cong |
| author_sort | Congqing Li |
| collection | DOAJ |
| description | Objective. Intrauterine adhesions affect menstruation and fertility, and endometrial fibrosis is the final manifestation of IUA. MMP-9 is closely related to fibrosis. The purpose of the study was to assess the role of MMP-9 in intrauterine adhesion (IUA) in rats and patients. Methods. 40 rats and 24 women were enrolled in this study. 40 rats were randomly divided into 3 groups: IUA group (n=20), sham group (n=10), and control group (n=10). Rat IUA models were established by intrauterine mechanical and chemical injured. In this study, 12 patients of intrauterine adhesions were detected and underwent TCRA (transcervical resection of adhesion) surgery, and endometrial tissue specimens were obtained during operation. One month later, an office hysteroscopy procedure was performed, and endometrial tissue specimens were obtained during operation again (postoperative group). A group of 12 normal age-matched control individuals served as controls underwent hysteroscopy and endometrial sampling. We used immunohistochemistry to detect MMP-9 expressions in rats and human endometrial tissues and to detect MMP-9 protein levels by Western blotting. In addition, we detected mRNA expression levels with qRT-PCR. Results. The expression of MMP-9 in the IUA rats was reduced compared with that in the sham group and Ctrl group (P<0.05), and the expression of MMP-9 was also reduced in the IUA patients compared with that in the Ctrl group (P<0.05). The mRNA levels of MMP-9 in the endometrium reflected similar results (P<0.05). The MMP-9 clearly increased even in the endometrium after TCRA surgery (P<0.05). Conclusion. Our study suggests that MMP-9 may play an important role in IUA. In the future, more in-depth research should be conducted on MMP-9. |
| format | Article |
| id | doaj-art-bac056cd5bc84abe8ae5bb816a084392 |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-bac056cd5bc84abe8ae5bb816a0843922025-08-20T03:22:53ZengWileyMediators of Inflammation0962-93511466-18612021-01-01202110.1155/2021/66765106676510Expression and Potential Role of MMP-9 in Intrauterine AdhesionCongqing Li0Wenyan Wang1Shiying Sun2Youjiang Xu3Ziang Fang4Lin Cong5Department of Obstetrics and Gynecology, The Second Hospital of Anhui Medical University, No. 678 Furong Road, Hefei, Anhui 230601, ChinaDepartment of Obstetrics and Gynecology, The Second Hospital of Anhui Medical University, No. 678 Furong Road, Hefei, Anhui 230601, ChinaDepartment of Obstetrics and Gynecology, The Second Hospital of Anhui Medical University, No. 678 Furong Road, Hefei, Anhui 230601, ChinaDepartment of Obstetrics and Gynecology, The Second Hospital of Anhui Medical University, No. 678 Furong Road, Hefei, Anhui 230601, ChinaDepartment of Obstetrics and Gynecology, The Second Hospital of Anhui Medical University, No. 678 Furong Road, Hefei, Anhui 230601, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei 230022, ChinaObjective. Intrauterine adhesions affect menstruation and fertility, and endometrial fibrosis is the final manifestation of IUA. MMP-9 is closely related to fibrosis. The purpose of the study was to assess the role of MMP-9 in intrauterine adhesion (IUA) in rats and patients. Methods. 40 rats and 24 women were enrolled in this study. 40 rats were randomly divided into 3 groups: IUA group (n=20), sham group (n=10), and control group (n=10). Rat IUA models were established by intrauterine mechanical and chemical injured. In this study, 12 patients of intrauterine adhesions were detected and underwent TCRA (transcervical resection of adhesion) surgery, and endometrial tissue specimens were obtained during operation. One month later, an office hysteroscopy procedure was performed, and endometrial tissue specimens were obtained during operation again (postoperative group). A group of 12 normal age-matched control individuals served as controls underwent hysteroscopy and endometrial sampling. We used immunohistochemistry to detect MMP-9 expressions in rats and human endometrial tissues and to detect MMP-9 protein levels by Western blotting. In addition, we detected mRNA expression levels with qRT-PCR. Results. The expression of MMP-9 in the IUA rats was reduced compared with that in the sham group and Ctrl group (P<0.05), and the expression of MMP-9 was also reduced in the IUA patients compared with that in the Ctrl group (P<0.05). The mRNA levels of MMP-9 in the endometrium reflected similar results (P<0.05). The MMP-9 clearly increased even in the endometrium after TCRA surgery (P<0.05). Conclusion. Our study suggests that MMP-9 may play an important role in IUA. In the future, more in-depth research should be conducted on MMP-9.http://dx.doi.org/10.1155/2021/6676510 |
| spellingShingle | Congqing Li Wenyan Wang Shiying Sun Youjiang Xu Ziang Fang Lin Cong Expression and Potential Role of MMP-9 in Intrauterine Adhesion Mediators of Inflammation |
| title | Expression and Potential Role of MMP-9 in Intrauterine Adhesion |
| title_full | Expression and Potential Role of MMP-9 in Intrauterine Adhesion |
| title_fullStr | Expression and Potential Role of MMP-9 in Intrauterine Adhesion |
| title_full_unstemmed | Expression and Potential Role of MMP-9 in Intrauterine Adhesion |
| title_short | Expression and Potential Role of MMP-9 in Intrauterine Adhesion |
| title_sort | expression and potential role of mmp 9 in intrauterine adhesion |
| url | http://dx.doi.org/10.1155/2021/6676510 |
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