Antibacterial mechanism of Lactiplantibacillus plantarum SHY96 cell-free supernatant against Listeria monocytogenes revealed by metabolomics and potential application on chicken breast meat preservation
The cell-free supernatant of Lactiplantibacillus plantarum (LCFS) is considered a potential natural antimicrobial agent due to its outstanding antimicrobial activity. This study demonstrated that the cell-free supernatant of L. plantarum SHY96 (LCFS96) effectively inhibits the growth and biofilm for...
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Elsevier
2025-01-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524009660 |
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| author | Ming Liang Hongwei Wang Zhaoquan Zhou Yechuan Huang Huayi Suo |
| author_facet | Ming Liang Hongwei Wang Zhaoquan Zhou Yechuan Huang Huayi Suo |
| author_sort | Ming Liang |
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| description | The cell-free supernatant of Lactiplantibacillus plantarum (LCFS) is considered a potential natural antimicrobial agent due to its outstanding antimicrobial activity. This study demonstrated that the cell-free supernatant of L. plantarum SHY96 (LCFS96) effectively inhibits the growth and biofilm formation of L. monocytogenes CMCC(B)54002 (L. monocytogenes_02) by reducing cell metabolic activity and damaging cell structure. Metabolomic analysis revealed that LCFS96 significantly altered 450 intracellular metabolites, affecting key metabolic pathways including linoleic acid metabolism, pyrimidine metabolism, purine metabolism, pantothenic acid and CoA biosynthesis, and the TCA cycle. Additionally, application of LCFS96 significantly reduced L. monocytogenes_02 viable counts by 84.93%, while maintaining the pH, TVB-N and organoleptic properties of chicken meat under refrigeration at 4 °C for 12 days. These findings highlight the antimicrobial mechanism and potential application of LCFS96 in extending the shelf-life of meat products. |
| format | Article |
| id | doaj-art-babba7dbb9f949f8a2c0dc3161156262 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-babba7dbb9f949f8a2c0dc31611562622025-02-12T05:31:52ZengElsevierFood Chemistry: X2590-15752025-01-0125102078Antibacterial mechanism of Lactiplantibacillus plantarum SHY96 cell-free supernatant against Listeria monocytogenes revealed by metabolomics and potential application on chicken breast meat preservationMing Liang0Hongwei Wang1Zhaoquan Zhou2Yechuan Huang3Huayi Suo4College of Food Science, Southwest University, Chongqing 400715, China; Chongqing Agricultural Product Processing Technology Innovation Platform, SouthwestUniversity, Chongqing 400715, ChinaCollege of Food Science, Southwest University, Chongqing 400715, China; Chongqing Agricultural Product Processing Technology Innovation Platform, SouthwestUniversity, Chongqing 400715, ChinaCollege of Food Science, Southwest University, Chongqing 400715, China; Chongqing Agricultural Product Processing Technology Innovation Platform, SouthwestUniversity, Chongqing 400715, ChinaCollege of Bioengineering, Jingchu University of Technology, Jingmen 448000, China; Corresponding author.College of Food Science, Southwest University, Chongqing 400715, China; Chongqing Agricultural Product Processing Technology Innovation Platform, SouthwestUniversity, Chongqing 400715, China; Corresponding author at: College of Food Science, Southwest University, Chongqing 400715, China.The cell-free supernatant of Lactiplantibacillus plantarum (LCFS) is considered a potential natural antimicrobial agent due to its outstanding antimicrobial activity. This study demonstrated that the cell-free supernatant of L. plantarum SHY96 (LCFS96) effectively inhibits the growth and biofilm formation of L. monocytogenes CMCC(B)54002 (L. monocytogenes_02) by reducing cell metabolic activity and damaging cell structure. Metabolomic analysis revealed that LCFS96 significantly altered 450 intracellular metabolites, affecting key metabolic pathways including linoleic acid metabolism, pyrimidine metabolism, purine metabolism, pantothenic acid and CoA biosynthesis, and the TCA cycle. Additionally, application of LCFS96 significantly reduced L. monocytogenes_02 viable counts by 84.93%, while maintaining the pH, TVB-N and organoleptic properties of chicken meat under refrigeration at 4 °C for 12 days. These findings highlight the antimicrobial mechanism and potential application of LCFS96 in extending the shelf-life of meat products.http://www.sciencedirect.com/science/article/pii/S2590157524009660Cell-free supernatantsLactiplantibacillus plantarumListeria monocytogenesMetabolomicsMeat product |
| spellingShingle | Ming Liang Hongwei Wang Zhaoquan Zhou Yechuan Huang Huayi Suo Antibacterial mechanism of Lactiplantibacillus plantarum SHY96 cell-free supernatant against Listeria monocytogenes revealed by metabolomics and potential application on chicken breast meat preservation Food Chemistry: X Cell-free supernatants Lactiplantibacillus plantarum Listeria monocytogenes Metabolomics Meat product |
| title | Antibacterial mechanism of Lactiplantibacillus plantarum SHY96 cell-free supernatant against Listeria monocytogenes revealed by metabolomics and potential application on chicken breast meat preservation |
| title_full | Antibacterial mechanism of Lactiplantibacillus plantarum SHY96 cell-free supernatant against Listeria monocytogenes revealed by metabolomics and potential application on chicken breast meat preservation |
| title_fullStr | Antibacterial mechanism of Lactiplantibacillus plantarum SHY96 cell-free supernatant against Listeria monocytogenes revealed by metabolomics and potential application on chicken breast meat preservation |
| title_full_unstemmed | Antibacterial mechanism of Lactiplantibacillus plantarum SHY96 cell-free supernatant against Listeria monocytogenes revealed by metabolomics and potential application on chicken breast meat preservation |
| title_short | Antibacterial mechanism of Lactiplantibacillus plantarum SHY96 cell-free supernatant against Listeria monocytogenes revealed by metabolomics and potential application on chicken breast meat preservation |
| title_sort | antibacterial mechanism of lactiplantibacillus plantarum shy96 cell free supernatant against listeria monocytogenes revealed by metabolomics and potential application on chicken breast meat preservation |
| topic | Cell-free supernatants Lactiplantibacillus plantarum Listeria monocytogenes Metabolomics Meat product |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524009660 |
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