Production, Characterization, and Antimicrobial Activity of Mycocin Produced by Debaryomyces hansenii DSMZ70238
The present study was conducted to estimate the antimicrobial activity and the potential biological control of the killer toxin produced by D. hansenii DSMZ70238 against several pathogenic microorganisms. In this study, the effects of NaCl, pH, and temperature, killer toxin production, and antimicro...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/2605382 |
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| author | Safaa A. S. Al-Qaysi Halah Al-Haideri Zaid Akram Thabit Wijdan Hameed Abd Al-Razzaq Al-Kubaisy Jamal Abd Al-Rahman Ibrahim |
| author_facet | Safaa A. S. Al-Qaysi Halah Al-Haideri Zaid Akram Thabit Wijdan Hameed Abd Al-Razzaq Al-Kubaisy Jamal Abd Al-Rahman Ibrahim |
| author_sort | Safaa A. S. Al-Qaysi |
| collection | DOAJ |
| description | The present study was conducted to estimate the antimicrobial activity and the potential biological control of the killer toxin produced by D. hansenii DSMZ70238 against several pathogenic microorganisms. In this study, the effects of NaCl, pH, and temperature, killer toxin production, and antimicrobial activity were studied. The results showed that the optimum inhibitory effect of killer toxin was at 8% NaCl, and the diameters of clear zones were 20, 22, 22, 21, 14, and 13 mm for Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes, Candida albicans, and Candida neoformans, respectively. The largest inhibition zones were observed at pH 4.5 with inhibition zone of 16, 18, 17, 18, 11, and 12 mm for the same microorganisms. The results also showed that 25°C is the optimal temperature for toxin killing activity against all targeted microorganisms. In addition, the activity of killer toxin significantly inhibited the growth of fungal mycelia for all target pathogenic fungi and the percentages of inhibition were 47.77, 48.88, 52.22, and 61.11% for Trichophyton rubrum, Alternaria alternata, Trichophyton concentricum, and Curvularia lunata, respectively. The results showed the highest growth rate of D. hansenii DSMZ70238 under condition of 8% NaCl concentration, pH 4.5, and 25°C for 72 h. |
| format | Article |
| id | doaj-art-bab5ddd704474cf4a8c0917f3fa6ed59 |
| institution | OA Journals |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-bab5ddd704474cf4a8c0917f3fa6ed592025-08-20T02:18:50ZengWileyInternational Journal of Microbiology1687-918X1687-91982017-01-01201710.1155/2017/26053822605382Production, Characterization, and Antimicrobial Activity of Mycocin Produced by Debaryomyces hansenii DSMZ70238Safaa A. S. Al-Qaysi0Halah Al-Haideri1Zaid Akram Thabit2Wijdan Hameed Abd Al-Razzaq Al-Kubaisy3Jamal Abd Al-Rahman Ibrahim4Department of Biology, College of Science for Women, University of Baghdad, Baghdad, IraqDepartment of Biology, College of Science for Women, University of Baghdad, Baghdad, IraqEnvironmental Biotechnology Department, Biotechnology Research Center, Al Nahrain University, Baghdad, IraqDepartment of Biology, College of Education for Pure Science, Al-Anbar University, Ramadi, IraqDepartment of Biology, College of Education for Pure Science, Al-Anbar University, Ramadi, IraqThe present study was conducted to estimate the antimicrobial activity and the potential biological control of the killer toxin produced by D. hansenii DSMZ70238 against several pathogenic microorganisms. In this study, the effects of NaCl, pH, and temperature, killer toxin production, and antimicrobial activity were studied. The results showed that the optimum inhibitory effect of killer toxin was at 8% NaCl, and the diameters of clear zones were 20, 22, 22, 21, 14, and 13 mm for Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes, Candida albicans, and Candida neoformans, respectively. The largest inhibition zones were observed at pH 4.5 with inhibition zone of 16, 18, 17, 18, 11, and 12 mm for the same microorganisms. The results also showed that 25°C is the optimal temperature for toxin killing activity against all targeted microorganisms. In addition, the activity of killer toxin significantly inhibited the growth of fungal mycelia for all target pathogenic fungi and the percentages of inhibition were 47.77, 48.88, 52.22, and 61.11% for Trichophyton rubrum, Alternaria alternata, Trichophyton concentricum, and Curvularia lunata, respectively. The results showed the highest growth rate of D. hansenii DSMZ70238 under condition of 8% NaCl concentration, pH 4.5, and 25°C for 72 h.http://dx.doi.org/10.1155/2017/2605382 |
| spellingShingle | Safaa A. S. Al-Qaysi Halah Al-Haideri Zaid Akram Thabit Wijdan Hameed Abd Al-Razzaq Al-Kubaisy Jamal Abd Al-Rahman Ibrahim Production, Characterization, and Antimicrobial Activity of Mycocin Produced by Debaryomyces hansenii DSMZ70238 International Journal of Microbiology |
| title | Production, Characterization, and Antimicrobial Activity of Mycocin Produced by Debaryomyces hansenii DSMZ70238 |
| title_full | Production, Characterization, and Antimicrobial Activity of Mycocin Produced by Debaryomyces hansenii DSMZ70238 |
| title_fullStr | Production, Characterization, and Antimicrobial Activity of Mycocin Produced by Debaryomyces hansenii DSMZ70238 |
| title_full_unstemmed | Production, Characterization, and Antimicrobial Activity of Mycocin Produced by Debaryomyces hansenii DSMZ70238 |
| title_short | Production, Characterization, and Antimicrobial Activity of Mycocin Produced by Debaryomyces hansenii DSMZ70238 |
| title_sort | production characterization and antimicrobial activity of mycocin produced by debaryomyces hansenii dsmz70238 |
| url | http://dx.doi.org/10.1155/2017/2605382 |
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