Circadian phase inversion causes insulin resistance in a rat model of night work and jet lag
Abstract Shift workers have an increased risk to develop type 2 diabetes. We aimed to investigate the underlying mechanisms and the role of the timing of food intake by subjecting rats to an acute phase inversion of the light/dark (L/D) cycle. In the first experiment, with food available ad libitum,...
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Nature Portfolio
2025-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-91485-z |
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| author | Anhui Wang Ewout Foppen Valentina S. Rumanova Tess Kool Andries Kalsbeek Dirk J. Stenvers |
| author_facet | Anhui Wang Ewout Foppen Valentina S. Rumanova Tess Kool Andries Kalsbeek Dirk J. Stenvers |
| author_sort | Anhui Wang |
| collection | DOAJ |
| description | Abstract Shift workers have an increased risk to develop type 2 diabetes. We aimed to investigate the underlying mechanisms and the role of the timing of food intake by subjecting rats to an acute phase inversion of the light/dark (L/D) cycle. In the first experiment, with food available ad libitum, male Wistar rats were implanted with jugular vein catheters and intravenous glucose tolerance tests were performed at either ZT2 or ZT14. Three days after the 12 h phase shift, these glucose tolerance tests were repeated. In the second experiment, rats were housed in metabolic cages for the continuous measurement of multiple behavioral and metabolic parameters after the 12 h phase shift, food was available ad libitum or restricted to the light or dark period. The daily rhythm of glucose tolerance, and the peak and trough corticosterone levels, adapted within three days after exposure to the inverted L/D cycle. However, phase inversion caused insulin resistance at the onset of the active phase. Under ad libitum feeding conditions, the daily rhythms of locomotor activity and energy expenditure adapted faster to the inverted L/D cycle compared to the other behavioral rhythms measured. Food restriction to the dark period facilitated behavioral adaptation to the new circadian phase. |
| format | Article |
| id | doaj-art-baafd47cc08746fa9de097c28e94e1ff |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-baafd47cc08746fa9de097c28e94e1ff2025-08-20T03:05:25ZengNature PortfolioScientific Reports2045-23222025-03-0115111210.1038/s41598-025-91485-zCircadian phase inversion causes insulin resistance in a rat model of night work and jet lagAnhui Wang0Ewout Foppen1Valentina S. Rumanova2Tess Kool3Andries Kalsbeek4Dirk J. Stenvers5Department of Endocrinology and Metabolism, Amsterdam UMC, University of AmsterdamLaboratory of Endocrinology, Department of Laboratory Medicine, Amsterdam UMC, University of AmsterdamDepartment of Endocrinology and Metabolism, Amsterdam UMC, University of AmsterdamDepartment of Endocrinology and Metabolism, Amsterdam UMC, University of AmsterdamDepartment of Endocrinology and Metabolism, Amsterdam UMC, University of AmsterdamDepartment of Endocrinology and Metabolism, Amsterdam UMC, University of AmsterdamAbstract Shift workers have an increased risk to develop type 2 diabetes. We aimed to investigate the underlying mechanisms and the role of the timing of food intake by subjecting rats to an acute phase inversion of the light/dark (L/D) cycle. In the first experiment, with food available ad libitum, male Wistar rats were implanted with jugular vein catheters and intravenous glucose tolerance tests were performed at either ZT2 or ZT14. Three days after the 12 h phase shift, these glucose tolerance tests were repeated. In the second experiment, rats were housed in metabolic cages for the continuous measurement of multiple behavioral and metabolic parameters after the 12 h phase shift, food was available ad libitum or restricted to the light or dark period. The daily rhythm of glucose tolerance, and the peak and trough corticosterone levels, adapted within three days after exposure to the inverted L/D cycle. However, phase inversion caused insulin resistance at the onset of the active phase. Under ad libitum feeding conditions, the daily rhythms of locomotor activity and energy expenditure adapted faster to the inverted L/D cycle compared to the other behavioral rhythms measured. Food restriction to the dark period facilitated behavioral adaptation to the new circadian phase.https://doi.org/10.1038/s41598-025-91485-zCircadian rhythmDaily rhythmsPhase-shiftDesynchronizationShift workMetabolism |
| spellingShingle | Anhui Wang Ewout Foppen Valentina S. Rumanova Tess Kool Andries Kalsbeek Dirk J. Stenvers Circadian phase inversion causes insulin resistance in a rat model of night work and jet lag Scientific Reports Circadian rhythm Daily rhythms Phase-shift Desynchronization Shift work Metabolism |
| title | Circadian phase inversion causes insulin resistance in a rat model of night work and jet lag |
| title_full | Circadian phase inversion causes insulin resistance in a rat model of night work and jet lag |
| title_fullStr | Circadian phase inversion causes insulin resistance in a rat model of night work and jet lag |
| title_full_unstemmed | Circadian phase inversion causes insulin resistance in a rat model of night work and jet lag |
| title_short | Circadian phase inversion causes insulin resistance in a rat model of night work and jet lag |
| title_sort | circadian phase inversion causes insulin resistance in a rat model of night work and jet lag |
| topic | Circadian rhythm Daily rhythms Phase-shift Desynchronization Shift work Metabolism |
| url | https://doi.org/10.1038/s41598-025-91485-z |
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