Characterisation and bioactivity of an exopolysaccharide isolated from Lactobacillus rhamnosus LRH30 cultivations
This study presents the first characterisation of the exopolysaccharide (EPS) produced by Lactobacillus rhamnosus LRH30 (L. rhamnosus LRH30) in skim milk-based bioprocesses. EPS yields varied significantly with cultivation mode, reaching 200.8 mg/L in shake flask, 427 mg/L in batch, and 1866 mg/L in...
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Elsevier
2025-06-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893925001951 |
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| author | Helena Mylise Copeland Paul Cannon Susan Maye George MacLeod Dermot Brabazon Christine Loscher Brian Freeland |
| author_facet | Helena Mylise Copeland Paul Cannon Susan Maye George MacLeod Dermot Brabazon Christine Loscher Brian Freeland |
| author_sort | Helena Mylise Copeland |
| collection | DOAJ |
| description | This study presents the first characterisation of the exopolysaccharide (EPS) produced by Lactobacillus rhamnosus LRH30 (L. rhamnosus LRH30) in skim milk-based bioprocesses. EPS yields varied significantly with cultivation mode, reaching 200.8 mg/L in shake flask, 427 mg/L in batch, and 1866 mg/L in fed-batch fermentation. When dissolved in milk, the EPS exhibited shear-thinning behaviour, indicating its suitability for texture enhancement in dairy applications. Morphological analysis via scanning electron microscopy (SEM) revealed a flaky, spherical microstructure. Fourier-transform infrared spectroscopy (FTIR) showed characteristic polysaccharide peaks, with similarities to commercial dextran. Monosaccharide composition analysis by high-performance liquid chromatography (HPLC) identified glucose and rhamnose as the dominant constituents. Bioactivity assays demonstrated dose-dependent immunomodulatory effects: EPS at 50 µg/mL significantly increased IL-10 and decreased TNF-α in LPS-stimulated macrophages. Additionally, IL-6 was reduced at 5 µg/mL, while IL-1β increased at 50 µg/mL. The EPS also exhibited moderate antioxidant activity, with a maximum radical scavenging capacity of 39.2 %. These findings highlight the potential of the EPS from L. rhamnosus LRH30 EPS as a novel, multifunctional ingredient for use in dairy-based functional foods. |
| format | Article |
| id | doaj-art-baa4c84c2abc460f985f92c83a5b7c60 |
| institution | DOAJ |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-baa4c84c2abc460f985f92c83a5b7c602025-08-20T03:20:58ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-06-011010085710.1016/j.carpta.2025.100857Characterisation and bioactivity of an exopolysaccharide isolated from Lactobacillus rhamnosus LRH30 cultivationsHelena Mylise Copeland0Paul Cannon1Susan Maye2George MacLeod3Dermot Brabazon4Christine Loscher5Brian Freeland6School of Biotechnology, Dublin City University, D9 Dublin, Ireland; I-Form, Advanced Manufacturing Research Centre, Dublin City University, D9 Dublin, Ireland; Corresponding author.School of Physics, Dublin City University, D9 Dublin, IrelandDairygold Co-Operative Society Limited, Clonmel Road, Co. Cork, P67 DD36, Mitchelstown, IrelandDairygold Co-Operative Society Limited, Clonmel Road, Co. Cork, P67 DD36, Mitchelstown, IrelandI-Form, Advanced Manufacturing Research Centre, Dublin City University, D9 Dublin, IrelandSchool of Biotechnology, Dublin City University, D9 Dublin, IrelandSchool of Biotechnology, Dublin City University, D9 Dublin, IrelandThis study presents the first characterisation of the exopolysaccharide (EPS) produced by Lactobacillus rhamnosus LRH30 (L. rhamnosus LRH30) in skim milk-based bioprocesses. EPS yields varied significantly with cultivation mode, reaching 200.8 mg/L in shake flask, 427 mg/L in batch, and 1866 mg/L in fed-batch fermentation. When dissolved in milk, the EPS exhibited shear-thinning behaviour, indicating its suitability for texture enhancement in dairy applications. Morphological analysis via scanning electron microscopy (SEM) revealed a flaky, spherical microstructure. Fourier-transform infrared spectroscopy (FTIR) showed characteristic polysaccharide peaks, with similarities to commercial dextran. Monosaccharide composition analysis by high-performance liquid chromatography (HPLC) identified glucose and rhamnose as the dominant constituents. Bioactivity assays demonstrated dose-dependent immunomodulatory effects: EPS at 50 µg/mL significantly increased IL-10 and decreased TNF-α in LPS-stimulated macrophages. Additionally, IL-6 was reduced at 5 µg/mL, while IL-1β increased at 50 µg/mL. The EPS also exhibited moderate antioxidant activity, with a maximum radical scavenging capacity of 39.2 %. These findings highlight the potential of the EPS from L. rhamnosus LRH30 EPS as a novel, multifunctional ingredient for use in dairy-based functional foods.http://www.sciencedirect.com/science/article/pii/S2666893925001951ExopolysaccharidemLactobacillusFunctional foodImmunomodulationCharacterisationDairy |
| spellingShingle | Helena Mylise Copeland Paul Cannon Susan Maye George MacLeod Dermot Brabazon Christine Loscher Brian Freeland Characterisation and bioactivity of an exopolysaccharide isolated from Lactobacillus rhamnosus LRH30 cultivations Carbohydrate Polymer Technologies and Applications Exopolysaccharidem Lactobacillus Functional food Immunomodulation Characterisation Dairy |
| title | Characterisation and bioactivity of an exopolysaccharide isolated from Lactobacillus rhamnosus LRH30 cultivations |
| title_full | Characterisation and bioactivity of an exopolysaccharide isolated from Lactobacillus rhamnosus LRH30 cultivations |
| title_fullStr | Characterisation and bioactivity of an exopolysaccharide isolated from Lactobacillus rhamnosus LRH30 cultivations |
| title_full_unstemmed | Characterisation and bioactivity of an exopolysaccharide isolated from Lactobacillus rhamnosus LRH30 cultivations |
| title_short | Characterisation and bioactivity of an exopolysaccharide isolated from Lactobacillus rhamnosus LRH30 cultivations |
| title_sort | characterisation and bioactivity of an exopolysaccharide isolated from lactobacillus rhamnosus lrh30 cultivations |
| topic | Exopolysaccharidem Lactobacillus Functional food Immunomodulation Characterisation Dairy |
| url | http://www.sciencedirect.com/science/article/pii/S2666893925001951 |
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