Arbuscular mycorrhizal fungi and biochar synergistically enhance Cd(II) phytoremediation in Koelreuteria bipinnata
Cadmium (Cd(II)) contamination is a global environmental issue. While synergistic interactions between arbuscular mycorrhizal fungi (AMF) and biochar may enhance Cd(II) phytoremediation in plants, the combined effects on Koelreuteria bipinnata and their underlying mechanisms remain unknown. To addre...
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Elsevier
2025-09-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325012278 |
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| author | Kang Liu Hongyan Chen Naili Zhang Lijia Dong Aiping Wu Xinping Wang Yanhong Wang |
| author_facet | Kang Liu Hongyan Chen Naili Zhang Lijia Dong Aiping Wu Xinping Wang Yanhong Wang |
| author_sort | Kang Liu |
| collection | DOAJ |
| description | Cadmium (Cd(II)) contamination is a global environmental issue. While synergistic interactions between arbuscular mycorrhizal fungi (AMF) and biochar may enhance Cd(II) phytoremediation in plants, the combined effects on Koelreuteria bipinnata and their underlying mechanisms remain unknown. To address this, we conducted a pot experiment assessing AMF inoculation (sterilized AMF, single or mixed inoculations of Rhizophagus irregularis and Diversispora versiformis) and rice-husk biochar amendment (0 % or 3 % substrate addition) on plant growth, soil properties, and Cd(II) uptake under varying soil Cd(II) concentrations (0, 50 and 150 mg kg−1). The results showed that Cd(II) exposure significantly reduced mycorrhizal colonization and biomass production, while increasing Cd(II) accumulation in soils and plant tissues. Individual AMF or biochar application enhanced plant growth and reduced Cd(II) uptake with distinct advantages: AMF alone excelled in boosting photosynthetic capability, biomass yield, and glomalin-related soil protein (GRSP) secretion; biochar alone more effectively promoted phosphorus absorption and decreased soil Cd(II) bioavailability. Critically, the dual-inoculation of R. irregularis and D. versiformis combined with biochar generated optimal remediation outcomes, achieving a 51 % reduction in shoot Cd(II) uptake, a 76 % decrease in soil total Cd(II) concentration, and a 74 % reduction in diethylenetriaminepentaacetic acid (DTPA)-extractable Cd(II) concentration. These synergistic effects were driven by enhanced spore density and GRSP secretion. Our findings demonstrate that combined R. irregularis-D. versiformis inoculation with biochar amendment provides an effective phytoremediation strategy for Cd(II)-contaminated soils using K. bipinnata. |
| format | Article |
| id | doaj-art-ba9fdfe55e4545649c4fc842768ee28d |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-ba9fdfe55e4545649c4fc842768ee28d2025-08-24T05:11:17ZengElsevierEcotoxicology and Environmental Safety0147-65132025-09-0130311888210.1016/j.ecoenv.2025.118882Arbuscular mycorrhizal fungi and biochar synergistically enhance Cd(II) phytoremediation in Koelreuteria bipinnataKang Liu0Hongyan Chen1Naili Zhang2Lijia Dong3Aiping Wu4Xinping Wang5Yanhong Wang6National Key Laboratory for Development and Utilization of Forest Food Resources, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, ChinaNational Key Laboratory for Development and Utilization of Forest Food Resources, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, ChinaState Key Laboratory of Efficient Production of Forest Resources and the Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, ChinaSchool of Life and Environmental Sciences, Shaoxing University, Shaoxing 312000, ChinaEcology Department, College of Environment and Ecology, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Hunan Agricultural University, Changsha 410128, ChinaNational Key Laboratory for Development and Utilization of Forest Food Resources, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, China; Corresponding author.National Key Laboratory for Development and Utilization of Forest Food Resources, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, China; Tianmushan Forest Ecosystem Orientation Observation and Research Station of Zhejiang Province, Hangzhou 311311, China; Corresponding author at: National Key Laboratory for Development and Utilization of Forest Food Resources, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, China.Cadmium (Cd(II)) contamination is a global environmental issue. While synergistic interactions between arbuscular mycorrhizal fungi (AMF) and biochar may enhance Cd(II) phytoremediation in plants, the combined effects on Koelreuteria bipinnata and their underlying mechanisms remain unknown. To address this, we conducted a pot experiment assessing AMF inoculation (sterilized AMF, single or mixed inoculations of Rhizophagus irregularis and Diversispora versiformis) and rice-husk biochar amendment (0 % or 3 % substrate addition) on plant growth, soil properties, and Cd(II) uptake under varying soil Cd(II) concentrations (0, 50 and 150 mg kg−1). The results showed that Cd(II) exposure significantly reduced mycorrhizal colonization and biomass production, while increasing Cd(II) accumulation in soils and plant tissues. Individual AMF or biochar application enhanced plant growth and reduced Cd(II) uptake with distinct advantages: AMF alone excelled in boosting photosynthetic capability, biomass yield, and glomalin-related soil protein (GRSP) secretion; biochar alone more effectively promoted phosphorus absorption and decreased soil Cd(II) bioavailability. Critically, the dual-inoculation of R. irregularis and D. versiformis combined with biochar generated optimal remediation outcomes, achieving a 51 % reduction in shoot Cd(II) uptake, a 76 % decrease in soil total Cd(II) concentration, and a 74 % reduction in diethylenetriaminepentaacetic acid (DTPA)-extractable Cd(II) concentration. These synergistic effects were driven by enhanced spore density and GRSP secretion. Our findings demonstrate that combined R. irregularis-D. versiformis inoculation with biochar amendment provides an effective phytoremediation strategy for Cd(II)-contaminated soils using K. bipinnata.http://www.sciencedirect.com/science/article/pii/S0147651325012278Cadmium pollutionArbuscular mycorrhizal fungiBiocharPhytoremediationKoelreuteria bipinnata |
| spellingShingle | Kang Liu Hongyan Chen Naili Zhang Lijia Dong Aiping Wu Xinping Wang Yanhong Wang Arbuscular mycorrhizal fungi and biochar synergistically enhance Cd(II) phytoremediation in Koelreuteria bipinnata Ecotoxicology and Environmental Safety Cadmium pollution Arbuscular mycorrhizal fungi Biochar Phytoremediation Koelreuteria bipinnata |
| title | Arbuscular mycorrhizal fungi and biochar synergistically enhance Cd(II) phytoremediation in Koelreuteria bipinnata |
| title_full | Arbuscular mycorrhizal fungi and biochar synergistically enhance Cd(II) phytoremediation in Koelreuteria bipinnata |
| title_fullStr | Arbuscular mycorrhizal fungi and biochar synergistically enhance Cd(II) phytoremediation in Koelreuteria bipinnata |
| title_full_unstemmed | Arbuscular mycorrhizal fungi and biochar synergistically enhance Cd(II) phytoremediation in Koelreuteria bipinnata |
| title_short | Arbuscular mycorrhizal fungi and biochar synergistically enhance Cd(II) phytoremediation in Koelreuteria bipinnata |
| title_sort | arbuscular mycorrhizal fungi and biochar synergistically enhance cd ii phytoremediation in koelreuteria bipinnata |
| topic | Cadmium pollution Arbuscular mycorrhizal fungi Biochar Phytoremediation Koelreuteria bipinnata |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325012278 |
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