Current cutting-edge omics techniques on musculoskeletal tissues and diseases
Abstract Musculoskeletal disorders, including osteoarthritis, rheumatoid arthritis, osteoporosis, bone fracture, intervertebral disc degeneration, tendinopathy, and myopathy, are prevalent conditions that profoundly impact quality of life and place substantial economic burdens on healthcare systems....
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2025-06-01
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| Series: | Bone Research |
| Online Access: | https://doi.org/10.1038/s41413-025-00442-z |
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| author | Xiaofei Li Liang Fang Renpeng Zhou Lutian Yao Sade W. Clayton Samantha Muscat Dakota R. Kamm Cuicui Wang Chuan-Ju Liu Ling Qin Robert J. Tower Courtney M. Karner Farshid Guilak Simon Y. Tang Alayna E. Loiselle Gretchen A. Meyer Jie Shen |
| author_facet | Xiaofei Li Liang Fang Renpeng Zhou Lutian Yao Sade W. Clayton Samantha Muscat Dakota R. Kamm Cuicui Wang Chuan-Ju Liu Ling Qin Robert J. Tower Courtney M. Karner Farshid Guilak Simon Y. Tang Alayna E. Loiselle Gretchen A. Meyer Jie Shen |
| author_sort | Xiaofei Li |
| collection | DOAJ |
| description | Abstract Musculoskeletal disorders, including osteoarthritis, rheumatoid arthritis, osteoporosis, bone fracture, intervertebral disc degeneration, tendinopathy, and myopathy, are prevalent conditions that profoundly impact quality of life and place substantial economic burdens on healthcare systems. Traditional bulk transcriptomics, genomics, proteomics, and metabolomics have played a pivotal role in uncovering disease-associated alterations at the population level. However, these approaches are inherently limited in their ability to resolve cellular heterogeneity or to capture the spatial organization of cells within tissues, thus hindering a comprehensive understanding of the complex cellular and molecular mechanisms underlying these diseases. To address these limitations, advanced single-cell and spatial omics techniques have emerged in recent years, offering unparalleled resolution for investigating cellular diversity, tissue microenvironments, and biomolecular interactions within musculoskeletal tissues. These cutting-edge techniques enable the detailed mapping of the molecular landscapes in diseased tissues, providing transformative insights into pathophysiological processes at both the single-cell and spatial levels. This review presents a comprehensive overview of the latest omics technologies as applied to musculoskeletal research, with a particular focus on their potential to revolutionize our understanding of disease mechanisms. Additionally, we explore the power of multi-omics integration in identifying novel therapeutic targets and highlight key challenges that must be overcome to successfully translate these advancements into clinical applications. |
| format | Article |
| id | doaj-art-ba9c2de01d384675bb96e19ae14a29e1 |
| institution | DOAJ |
| issn | 2095-6231 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Bone Research |
| spelling | doaj-art-ba9c2de01d384675bb96e19ae14a29e12025-08-20T02:39:45ZengNature Publishing GroupBone Research2095-62312025-06-0113113010.1038/s41413-025-00442-zCurrent cutting-edge omics techniques on musculoskeletal tissues and diseasesXiaofei Li0Liang Fang1Renpeng Zhou2Lutian Yao3Sade W. Clayton4Samantha Muscat5Dakota R. Kamm6Cuicui Wang7Chuan-Ju Liu8Ling Qin9Robert J. Tower10Courtney M. Karner11Farshid Guilak12Simon Y. Tang13Alayna E. Loiselle14Gretchen A. Meyer15Jie Shen16Department of Orthopaedic Surgery, Washington UniversityDepartment of Orthopaedic Surgery, Washington UniversityDepartment of Orthopaedics and Rehabilitation, Yale UniversityDepartment of Orthopaedic Surgery, University of PennsylvaniaDepartment of Orthopaedic Surgery, Washington UniversityDepartment of Pathology & Laboratory Medicine, University of Rochester Medical CenterProgram in Physical Therapy, Washington UniversityDepartment of Orthopaedic Surgery, Washington UniversityDepartment of Orthopaedics and Rehabilitation, Yale UniversityDepartment of Orthopaedic Surgery, University of PennsylvaniaDepartment of Surgery, UT Southwestern Medical CenterDepartment of Internal Medicine, UT Southwestern Medical CenterDepartment of Orthopaedic Surgery, Washington UniversityDepartment of Orthopaedic Surgery, Washington UniversityDepartment of Pathology & Laboratory Medicine, University of Rochester Medical CenterDepartment of Orthopaedic Surgery, Washington UniversityDepartment of Orthopaedic Surgery, Washington UniversityAbstract Musculoskeletal disorders, including osteoarthritis, rheumatoid arthritis, osteoporosis, bone fracture, intervertebral disc degeneration, tendinopathy, and myopathy, are prevalent conditions that profoundly impact quality of life and place substantial economic burdens on healthcare systems. Traditional bulk transcriptomics, genomics, proteomics, and metabolomics have played a pivotal role in uncovering disease-associated alterations at the population level. However, these approaches are inherently limited in their ability to resolve cellular heterogeneity or to capture the spatial organization of cells within tissues, thus hindering a comprehensive understanding of the complex cellular and molecular mechanisms underlying these diseases. To address these limitations, advanced single-cell and spatial omics techniques have emerged in recent years, offering unparalleled resolution for investigating cellular diversity, tissue microenvironments, and biomolecular interactions within musculoskeletal tissues. These cutting-edge techniques enable the detailed mapping of the molecular landscapes in diseased tissues, providing transformative insights into pathophysiological processes at both the single-cell and spatial levels. This review presents a comprehensive overview of the latest omics technologies as applied to musculoskeletal research, with a particular focus on their potential to revolutionize our understanding of disease mechanisms. Additionally, we explore the power of multi-omics integration in identifying novel therapeutic targets and highlight key challenges that must be overcome to successfully translate these advancements into clinical applications.https://doi.org/10.1038/s41413-025-00442-z |
| spellingShingle | Xiaofei Li Liang Fang Renpeng Zhou Lutian Yao Sade W. Clayton Samantha Muscat Dakota R. Kamm Cuicui Wang Chuan-Ju Liu Ling Qin Robert J. Tower Courtney M. Karner Farshid Guilak Simon Y. Tang Alayna E. Loiselle Gretchen A. Meyer Jie Shen Current cutting-edge omics techniques on musculoskeletal tissues and diseases Bone Research |
| title | Current cutting-edge omics techniques on musculoskeletal tissues and diseases |
| title_full | Current cutting-edge omics techniques on musculoskeletal tissues and diseases |
| title_fullStr | Current cutting-edge omics techniques on musculoskeletal tissues and diseases |
| title_full_unstemmed | Current cutting-edge omics techniques on musculoskeletal tissues and diseases |
| title_short | Current cutting-edge omics techniques on musculoskeletal tissues and diseases |
| title_sort | current cutting edge omics techniques on musculoskeletal tissues and diseases |
| url | https://doi.org/10.1038/s41413-025-00442-z |
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