Quantifying hygroscopic deformation in lignocellulosic tissues: a digital volume correlation tool comparison

Digital Volume Correlation (DVC) was used to study the hygroscopic shrinkage in lignocellulosic tissues. For this, small tissue segments of only a few cells were prepared from the endocarp of Hura crepitans fruits, the sclereid cell layer of Pinus jeffreyi pine cone scales, the sclerenchyma fiber sh...

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Main Authors: Kim Ulrich, Fabian Scheckenbach, Tak Ming Wong, Tom Masselter, Silja Flenner, Anaclara Visconti, Martin Nopens, Andreas Krause, Sergej Kaschuro, Jakob Benedikt Mietner, Thomas Speck, Imke Greving, Berit Zeller-Plumhoff, Linnea Hesse
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-08-01
Series:Frontiers in Plant Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2025.1572745/full
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author Kim Ulrich
Kim Ulrich
Fabian Scheckenbach
Tak Ming Wong
Tak Ming Wong
Tom Masselter
Silja Flenner
Anaclara Visconti
Anaclara Visconti
Anaclara Visconti
Martin Nopens
Andreas Krause
Sergej Kaschuro
Jakob Benedikt Mietner
Thomas Speck
Thomas Speck
Imke Greving
Berit Zeller-Plumhoff
Berit Zeller-Plumhoff
Linnea Hesse
Linnea Hesse
author_facet Kim Ulrich
Kim Ulrich
Fabian Scheckenbach
Tak Ming Wong
Tak Ming Wong
Tom Masselter
Silja Flenner
Anaclara Visconti
Anaclara Visconti
Anaclara Visconti
Martin Nopens
Andreas Krause
Sergej Kaschuro
Jakob Benedikt Mietner
Thomas Speck
Thomas Speck
Imke Greving
Berit Zeller-Plumhoff
Berit Zeller-Plumhoff
Linnea Hesse
Linnea Hesse
author_sort Kim Ulrich
collection DOAJ
description Digital Volume Correlation (DVC) was used to study the hygroscopic shrinkage in lignocellulosic tissues. For this, small tissue segments of only a few cells were prepared from the endocarp of Hura crepitans fruits, the sclereid cell layer of Pinus jeffreyi pine cone scales, the sclerenchyma fiber sheath of peripheral vascular bundles in Marantochloa leucantha and latewood of Pinus sylvestris. The cells were imaged in a wet and dry state using X-ray nano-holotomography. Subsequently, a DVC analysis was conducted using Avizo™, elastix and MBS-3D-OptFlow, to visualize and quantify their hygroscopic shrinkage and to compare the accuracy of the approaches. The results reveal an anisotropic shrinkage behavior (1) along the cell length compared to radial shrinkage and (2) a greater radial than tangential shrinkage within the cell wall. The accuracy of the DVC results was validated and compared using two artificially deformed datasets (linear and sinusoidal) for controls. A (sub-)voxel accuracy for both controls could be demonstrated for each software with the image registration toolkit elastix performing best. In addition, the abundance of structural features in the cell walls leads to an improved DVC accuracy. Overall, DVC proved to be a viable approach to study the hygroscopic deformation of lignocellulosic tissue samples.
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spelling doaj-art-ba97cd58b0d34d2c9e1a8d8ecc5fd5052025-08-20T04:02:51ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-08-011610.3389/fpls.2025.15727451572745Quantifying hygroscopic deformation in lignocellulosic tissues: a digital volume correlation tool comparisonKim Ulrich0Kim Ulrich1Fabian Scheckenbach2Tak Ming Wong3Tak Ming Wong4Tom Masselter5Silja Flenner6Anaclara Visconti7Anaclara Visconti8Anaclara Visconti9Martin Nopens10Andreas Krause11Sergej Kaschuro12Jakob Benedikt Mietner13Thomas Speck14Thomas Speck15Imke Greving16Berit Zeller-Plumhoff17Berit Zeller-Plumhoff18Linnea Hesse19Linnea Hesse20Plant Biomechanics Group @ Botanic Garden, University of Freiburg, Freiburg im Breisgau, GermanyCluster of Excellence livMatS @ FIT—Freiburg Center for Interactive Materials and Bioinspired Technologies, University of Freiburg, Freiburg im Breisgau, GermanyBiomimetics Group, Institute of Wood Sciences, University of Hamburg, Hamburg, GermanyInstitute of Metallic Biomaterials, Helmholtz-Zentrum Hereon, Geesthacht, GermanyInstitute of Materials Physics, Helmholtz-Zentrum Hereon, Geesthacht, GermanyPlant Biomechanics Group @ Botanic Garden, University of Freiburg, Freiburg im Breisgau, GermanyInstitute of Materials Physics, Helmholtz-Zentrum Hereon, Geesthacht, GermanyPlant Biomechanics Group @ Botanic Garden, University of Freiburg, Freiburg im Breisgau, GermanyBiomimetics Group, Institute of Wood Sciences, University of Hamburg, Hamburg, GermanyResearch Area Landscape Functioning, Leibniz Center for Agricultural Landscape Research (ZALF) e.V., Müncheberg, GermanyThünen Institute of Wood Research, Thünen Institute, Hamburg, GermanyThünen Institute of Wood Research, Thünen Institute, Hamburg, GermanyThünen Institute of Wood Research, Thünen Institute, Hamburg, GermanyBiomimetics Group, Institute of Wood Sciences, University of Hamburg, Hamburg, GermanyPlant Biomechanics Group @ Botanic Garden, University of Freiburg, Freiburg im Breisgau, GermanyCluster of Excellence livMatS @ FIT—Freiburg Center for Interactive Materials and Bioinspired Technologies, University of Freiburg, Freiburg im Breisgau, GermanyInstitute of Materials Physics, Helmholtz-Zentrum Hereon, Geesthacht, GermanyInstitute of Metallic Biomaterials, Helmholtz-Zentrum Hereon, Geesthacht, GermanyData-driven Analysis and Design of Materials, Faculty of Mechanical Engineering and Marine Technologies, University of Rostock, Rostock, GermanyCluster of Excellence livMatS @ FIT—Freiburg Center for Interactive Materials and Bioinspired Technologies, University of Freiburg, Freiburg im Breisgau, GermanyBiomimetics Group, Institute of Wood Sciences, University of Hamburg, Hamburg, GermanyDigital Volume Correlation (DVC) was used to study the hygroscopic shrinkage in lignocellulosic tissues. For this, small tissue segments of only a few cells were prepared from the endocarp of Hura crepitans fruits, the sclereid cell layer of Pinus jeffreyi pine cone scales, the sclerenchyma fiber sheath of peripheral vascular bundles in Marantochloa leucantha and latewood of Pinus sylvestris. The cells were imaged in a wet and dry state using X-ray nano-holotomography. Subsequently, a DVC analysis was conducted using Avizo™, elastix and MBS-3D-OptFlow, to visualize and quantify their hygroscopic shrinkage and to compare the accuracy of the approaches. The results reveal an anisotropic shrinkage behavior (1) along the cell length compared to radial shrinkage and (2) a greater radial than tangential shrinkage within the cell wall. The accuracy of the DVC results was validated and compared using two artificially deformed datasets (linear and sinusoidal) for controls. A (sub-)voxel accuracy for both controls could be demonstrated for each software with the image registration toolkit elastix performing best. In addition, the abundance of structural features in the cell walls leads to an improved DVC accuracy. Overall, DVC proved to be a viable approach to study the hygroscopic deformation of lignocellulosic tissue samples.https://www.frontiersin.org/articles/10.3389/fpls.2025.1572745/fullbiomechanicswood sciencelignocelluloseDVChygroscopycomputed tomography
spellingShingle Kim Ulrich
Kim Ulrich
Fabian Scheckenbach
Tak Ming Wong
Tak Ming Wong
Tom Masselter
Silja Flenner
Anaclara Visconti
Anaclara Visconti
Anaclara Visconti
Martin Nopens
Andreas Krause
Sergej Kaschuro
Jakob Benedikt Mietner
Thomas Speck
Thomas Speck
Imke Greving
Berit Zeller-Plumhoff
Berit Zeller-Plumhoff
Linnea Hesse
Linnea Hesse
Quantifying hygroscopic deformation in lignocellulosic tissues: a digital volume correlation tool comparison
Frontiers in Plant Science
biomechanics
wood science
lignocellulose
DVC
hygroscopy
computed tomography
title Quantifying hygroscopic deformation in lignocellulosic tissues: a digital volume correlation tool comparison
title_full Quantifying hygroscopic deformation in lignocellulosic tissues: a digital volume correlation tool comparison
title_fullStr Quantifying hygroscopic deformation in lignocellulosic tissues: a digital volume correlation tool comparison
title_full_unstemmed Quantifying hygroscopic deformation in lignocellulosic tissues: a digital volume correlation tool comparison
title_short Quantifying hygroscopic deformation in lignocellulosic tissues: a digital volume correlation tool comparison
title_sort quantifying hygroscopic deformation in lignocellulosic tissues a digital volume correlation tool comparison
topic biomechanics
wood science
lignocellulose
DVC
hygroscopy
computed tomography
url https://www.frontiersin.org/articles/10.3389/fpls.2025.1572745/full
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