T-DNAreader: fast and precise identification of T-DNA insertion sites in plant genomes using RNA sequencing data

Abstract Agrobacterium-mediated plant transformation, which enables the delivery of DNA using transfer DNA (T-DNA) binary vectors, is an essential technique in plant research. T-DNAs randomly integrate into the host genome, and multiple T-DNAs can sometimes integrate during a single transformation,...

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Bibliographic Details
Main Authors: Hongwoo Lee, Pil Joon Seo
Format: Article
Language:English
Published: BMC 2025-07-01
Series:Genome Biology
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Online Access:https://doi.org/10.1186/s13059-025-03655-x
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