Blood plasma lipid profile in glial tumors
Introduction. In glial tumors, lipid metabolism becomes abnormal. Analysis of lipid metabolism components can be an important characteristic of molecular and genetic profile of gliomas.Aim. To determine the correlation between plasma lipidome profile and immunohistochemical characteristics of glial...
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ABV-press
2024-10-01
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| Series: | Успехи молекулярной онкологии |
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| Online Access: | https://umo.abvpress.ru/jour/article/view/713 |
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| author | L. M. Obukhova E. V. Balavina T. A. Veselova I. A. Medyanik A. S. Grishin V. F. Lazukin M. M. Kontorshchikov |
| author_facet | L. M. Obukhova E. V. Balavina T. A. Veselova I. A. Medyanik A. S. Grishin V. F. Lazukin M. M. Kontorshchikov |
| author_sort | L. M. Obukhova |
| collection | DOAJ |
| description | Introduction. In glial tumors, lipid metabolism becomes abnormal. Analysis of lipid metabolism components can be an important characteristic of molecular and genetic profile of gliomas.Aim. To determine the correlation between plasma lipidome profile and immunohistochemical characteristics of glial tumors and to evaluate clinical significance of blood lipid spectrum analysis in preoperative assessment of molecular profile of gliomas.Materials and methods. Immunohistochemical measurement of O-6-methylguanine-DNA-methyl transferase (MGMT), Ki-67, p53, IDH1 tumor markers was performed using the corresponding antibody clones. Composition of plasma lipids was assessed using thin layer chromatography.Results. Even at the early stages of gliomagenesis, significant differences in cholesterol ethers, lysophosphatidylcholines, phosphatidylcholine (PC)/ lysophosphatidylcholine (LPC) ratio, neutral lipids (NL)/phospholipids (PL) in the blood were observed. Significant correlations between Ki-67, MGMT tumor markers and the above-mentioned lipidome parameters were found. The PC/LPC, NL/PL ratios in the blood of the patients from the groups with higher (above 10 %) and lower (below 10 %) Ki-67 mitotic indexes compared to healthy individuals were significantly lower. Therefore, the values of lipidome parameters allow to indirectly assess proliferative activity of gliomas which can be used for preoperative diagnosis of these tumors. No significant differences in the plasma PC/LPC and NL/PL ratios were found between the groups with MGMT promoter methylation and without it. No indirect predictor criteria for MGMT were found.Conclusion. It is impossible to determine decreased epigenetic activity of corresponding transcripts and preoperative prognosis for alkylating agent therapy based on the parameters of plasma lipid metabolism. |
| format | Article |
| id | doaj-art-ba94cadb00ac4c00979c09ee88eebf62 |
| institution | Kabale University |
| issn | 2313-805X 2413-3787 |
| language | Russian |
| publishDate | 2024-10-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Успехи молекулярной онкологии |
| spelling | doaj-art-ba94cadb00ac4c00979c09ee88eebf622025-08-20T04:00:15ZrusABV-pressУспехи молекулярной онкологии2313-805X2413-37872024-10-0111311412510.17650/2313-805X-2024-11-3-114-125344Blood plasma lipid profile in glial tumorsL. M. Obukhova0E. V. Balavina1T. A. Veselova2I. A. Medyanik3A. S. Grishin4V. F. Lazukin5M. M. Kontorshchikov6Privolzhsky Research Medical University, Ministry of Health of RussiaNational Research Lobachevsky State University of Nizhny NovgorodNational Research Lobachevsky State University of Nizhny NovgorodPrivolzhsky Research Medical University, Ministry of Health of RussiaPrivolzhsky Research Medical University, Ministry of Health of RussiaPrivolzhsky Research Medical University, Ministry of Health of RussiaPrivolzhsky Research Medical University, Ministry of Health of RussiaIntroduction. In glial tumors, lipid metabolism becomes abnormal. Analysis of lipid metabolism components can be an important characteristic of molecular and genetic profile of gliomas.Aim. To determine the correlation between plasma lipidome profile and immunohistochemical characteristics of glial tumors and to evaluate clinical significance of blood lipid spectrum analysis in preoperative assessment of molecular profile of gliomas.Materials and methods. Immunohistochemical measurement of O-6-methylguanine-DNA-methyl transferase (MGMT), Ki-67, p53, IDH1 tumor markers was performed using the corresponding antibody clones. Composition of plasma lipids was assessed using thin layer chromatography.Results. Even at the early stages of gliomagenesis, significant differences in cholesterol ethers, lysophosphatidylcholines, phosphatidylcholine (PC)/ lysophosphatidylcholine (LPC) ratio, neutral lipids (NL)/phospholipids (PL) in the blood were observed. Significant correlations between Ki-67, MGMT tumor markers and the above-mentioned lipidome parameters were found. The PC/LPC, NL/PL ratios in the blood of the patients from the groups with higher (above 10 %) and lower (below 10 %) Ki-67 mitotic indexes compared to healthy individuals were significantly lower. Therefore, the values of lipidome parameters allow to indirectly assess proliferative activity of gliomas which can be used for preoperative diagnosis of these tumors. No significant differences in the plasma PC/LPC and NL/PL ratios were found between the groups with MGMT promoter methylation and without it. No indirect predictor criteria for MGMT were found.Conclusion. It is impossible to determine decreased epigenetic activity of corresponding transcripts and preoperative prognosis for alkylating agent therapy based on the parameters of plasma lipid metabolism.https://umo.abvpress.ru/jour/article/view/713blood plasmalipidcholesterol esterphosphatidylcholine/lysophosphatidylcholine ratioand neutral lipids/phospholipids ratiomolecular genetic marker of gliomaidh1mgmtki-67p53glioma |
| spellingShingle | L. M. Obukhova E. V. Balavina T. A. Veselova I. A. Medyanik A. S. Grishin V. F. Lazukin M. M. Kontorshchikov Blood plasma lipid profile in glial tumors Успехи молекулярной онкологии blood plasma lipid cholesterol ester phosphatidylcholine/lysophosphatidylcholine ratio and neutral lipids/phospholipids ratio molecular genetic marker of glioma idh1 mgmt ki-67 p53 glioma |
| title | Blood plasma lipid profile in glial tumors |
| title_full | Blood plasma lipid profile in glial tumors |
| title_fullStr | Blood plasma lipid profile in glial tumors |
| title_full_unstemmed | Blood plasma lipid profile in glial tumors |
| title_short | Blood plasma lipid profile in glial tumors |
| title_sort | blood plasma lipid profile in glial tumors |
| topic | blood plasma lipid cholesterol ester phosphatidylcholine/lysophosphatidylcholine ratio and neutral lipids/phospholipids ratio molecular genetic marker of glioma idh1 mgmt ki-67 p53 glioma |
| url | https://umo.abvpress.ru/jour/article/view/713 |
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