Aspergillus awamori MH2 as a novel maltobionic acid producer: production optimization and application
Abstract Background Producing bio-based chemicals using a straightforward and ecologically responsible biotechnological method is intriguing. Maltobionic acid (MBA) is an aldobionic acid obtained from maltose oxidation and is an industrially applied compound. Having antioxidative and antimicrobial,...
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BMC
2025-08-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-025-02804-y |
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| author | Soad M. Yehia Yousseria M. Shetaia Ghoson M. Daba Faten A. Mostafa Ali A. Ali Hassaan El-Menoufy |
| author_facet | Soad M. Yehia Yousseria M. Shetaia Ghoson M. Daba Faten A. Mostafa Ali A. Ali Hassaan El-Menoufy |
| author_sort | Soad M. Yehia |
| collection | DOAJ |
| description | Abstract Background Producing bio-based chemicals using a straightforward and ecologically responsible biotechnological method is intriguing. Maltobionic acid (MBA) is an aldobionic acid obtained from maltose oxidation and is an industrially applied compound. Having antioxidative and antimicrobial, non-toxic, highly water soluble, moisturizing, metal chelating, mildly sour and slightly sweet characteristics. Results This study succeeded in utilizing Aspergillus awamori as a novel MBA producer. MBA production was improved through two-step statistical factorial designs. Plackett-Burman Design (PBD) investigating the qualitative interaction between eleven factors (maltose, KNO3, NaCl, KH2PO4, K2HPO4, MgSO4, FeSO4.7H2O, initial pH, temperature, incubation time and rpm) on MBA production causing 1.37- fold increase. Central Composite design (CCD) analyzing the quantitative interaction between the most MBA production affecting factors (temperature, rpm, KH2PO4, and incubation period) gave a 1.64- improvement in MBA production compared with un-optimized medium. The addition of agro-industrial wastes (AIW) (corn cobs and artichoke leaves) to optimized medium g/l (maltose, 5; KNO3, 1; KH2PO4, 1.0; MgSO4, 0.5; FeSO4.7(H2O), 0.02; pH, 5.00 at 37.5 °C for 9.5 days at 125 rpm) caused 2.41- and 1.97- fold increase in MBA production, respectively in comparison with the initial production conditions. MBA produced by A. awamori MH2 exerted an anti-oxidant activity with a ratio of 86% using DPPH scavenging assay. |
| format | Article |
| id | doaj-art-ba8f77f774474bfa9bf35c3a32d69a02 |
| institution | Kabale University |
| issn | 1475-2859 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj-art-ba8f77f774474bfa9bf35c3a32d69a022025-08-20T03:46:37ZengBMCMicrobial Cell Factories1475-28592025-08-0124111010.1186/s12934-025-02804-yAspergillus awamori MH2 as a novel maltobionic acid producer: production optimization and applicationSoad M. Yehia0Yousseria M. Shetaia1Ghoson M. Daba2Faten A. Mostafa3Ali A. Ali4Hassaan El-Menoufy5Department of Microbiology, Faculty of Science, Ain Shams UniversityDepartment of Microbiology, Faculty of Science, Ain Shams UniversityChemistry of Natural and Microbial products Department, National Research CentreChemistry of Natural and Microbial products Department, National Research CentreFood Sciences Department, Faculty of Agriculture, Ain Shams UniversityChemistry of Natural and Microbial products Department, National Research CentreAbstract Background Producing bio-based chemicals using a straightforward and ecologically responsible biotechnological method is intriguing. Maltobionic acid (MBA) is an aldobionic acid obtained from maltose oxidation and is an industrially applied compound. Having antioxidative and antimicrobial, non-toxic, highly water soluble, moisturizing, metal chelating, mildly sour and slightly sweet characteristics. Results This study succeeded in utilizing Aspergillus awamori as a novel MBA producer. MBA production was improved through two-step statistical factorial designs. Plackett-Burman Design (PBD) investigating the qualitative interaction between eleven factors (maltose, KNO3, NaCl, KH2PO4, K2HPO4, MgSO4, FeSO4.7H2O, initial pH, temperature, incubation time and rpm) on MBA production causing 1.37- fold increase. Central Composite design (CCD) analyzing the quantitative interaction between the most MBA production affecting factors (temperature, rpm, KH2PO4, and incubation period) gave a 1.64- improvement in MBA production compared with un-optimized medium. The addition of agro-industrial wastes (AIW) (corn cobs and artichoke leaves) to optimized medium g/l (maltose, 5; KNO3, 1; KH2PO4, 1.0; MgSO4, 0.5; FeSO4.7(H2O), 0.02; pH, 5.00 at 37.5 °C for 9.5 days at 125 rpm) caused 2.41- and 1.97- fold increase in MBA production, respectively in comparison with the initial production conditions. MBA produced by A. awamori MH2 exerted an anti-oxidant activity with a ratio of 86% using DPPH scavenging assay.https://doi.org/10.1186/s12934-025-02804-yMaltobionic acidAspergillus awamoriPlackett-Burman designCentral composite designAgro-industrial wastes |
| spellingShingle | Soad M. Yehia Yousseria M. Shetaia Ghoson M. Daba Faten A. Mostafa Ali A. Ali Hassaan El-Menoufy Aspergillus awamori MH2 as a novel maltobionic acid producer: production optimization and application Microbial Cell Factories Maltobionic acid Aspergillus awamori Plackett-Burman design Central composite design Agro-industrial wastes |
| title | Aspergillus awamori MH2 as a novel maltobionic acid producer: production optimization and application |
| title_full | Aspergillus awamori MH2 as a novel maltobionic acid producer: production optimization and application |
| title_fullStr | Aspergillus awamori MH2 as a novel maltobionic acid producer: production optimization and application |
| title_full_unstemmed | Aspergillus awamori MH2 as a novel maltobionic acid producer: production optimization and application |
| title_short | Aspergillus awamori MH2 as a novel maltobionic acid producer: production optimization and application |
| title_sort | aspergillus awamori mh2 as a novel maltobionic acid producer production optimization and application |
| topic | Maltobionic acid Aspergillus awamori Plackett-Burman design Central composite design Agro-industrial wastes |
| url | https://doi.org/10.1186/s12934-025-02804-y |
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