Reconstruction of Single-Neuron Projectomes in Mice
Reconstructing single-neuron projectomes is essential for mapping the mesoscopic connectome and eventually for understanding brain-wide connectivity and diverse brain functions. The combination of sparse labeling techniques and large-scale and high-resolution optical imaging technologies has been re...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2025-05-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5300&type=0 |
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| Summary: | Reconstructing single-neuron projectomes is essential for mapping the mesoscopic connectome and eventually for understanding brain-wide connectivity and diverse brain functions. The combination of sparse labeling techniques and large-scale and high-resolution optical imaging technologies has been revolutionizing the brain-wide reconstruction of single-neuron morphologies, as exemplified by the dataset for over 10,100 single-neuron projectomes of hippocampal neurons. Here, we illustrate a comprehensive protocol for large-scale single-neuron reconstruction in the mouse brain. This includes key steps and examples in imaging data preprocessing, neurite tracing, and registration into a template brain. These procedures enable efficient and accurate large-scale morphological reconstruction of single neurons in the mouse brain. |
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| ISSN: | 2331-8325 |