Elongation factor Tu promotes the onset of periodontitis through mediating bacteria adhesion
Abstract Periodontitis, a leading cause of adult tooth loss and linked to various systemic diseases, is promoted by subgingival plaque biofilms, with Streptococci as early colonizers responsible for surface adhesion. Current studies of Streptococci adhesion have focused on bacteria surface adhesins...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-03-01
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| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-025-00680-3 |
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| _version_ | 1849390330251575296 |
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| author | Leyi Xiao Yingying Pu Yu Cui Chen Chen Qi Xiao Yulan Wang Yan Wei Mengge Feng Tiange Zhang Shanyi Yang Jingxuan Zhou Yueqi Ni Jinglun Zhang Hebin Liao Jingwen Wu Yufeng Zhang |
| author_facet | Leyi Xiao Yingying Pu Yu Cui Chen Chen Qi Xiao Yulan Wang Yan Wei Mengge Feng Tiange Zhang Shanyi Yang Jingxuan Zhou Yueqi Ni Jinglun Zhang Hebin Liao Jingwen Wu Yufeng Zhang |
| author_sort | Leyi Xiao |
| collection | DOAJ |
| description | Abstract Periodontitis, a leading cause of adult tooth loss and linked to various systemic diseases, is promoted by subgingival plaque biofilms, with Streptococci as early colonizers responsible for surface adhesion. Current studies of Streptococci adhesion have focused on bacteria surface adhesins with acquired protein membranes on the tooth surface, yet no critical proteins with implications for the overall early adhesion of subgingival plaque have been reported. Here, we identified that the “Barrel-like adhesion domain” of streptococcal EF-Tu facilitates cell-surface attachment, promotes biofilm formation, and contributes to the development of periodontitis. In the adherent state, EF-Tu is transported from the cytoplasm to the cell surface through membrane vesicles. Furthermore, we first found that simeprevir, an FDA-approved drug, binds to the “Barrel-like adhesion domain” of EF-Tu and effectively inhibits the protein’s surface adhesion and secretory pathways. Simeprevir showed the ability to inhibit dental plaque formation and provided prevention and treatments for periodontitis. |
| format | Article |
| id | doaj-art-ba8723ef9eae477abff596a617092eb1 |
| institution | Kabale University |
| issn | 2055-5008 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj-art-ba8723ef9eae477abff596a617092eb12025-08-20T03:41:41ZengNature Portfolionpj Biofilms and Microbiomes2055-50082025-03-0111111510.1038/s41522-025-00680-3Elongation factor Tu promotes the onset of periodontitis through mediating bacteria adhesionLeyi Xiao0Yingying Pu1Yu Cui2Chen Chen3Qi Xiao4Yulan Wang5Yan Wei6Mengge Feng7Tiange Zhang8Shanyi Yang9Jingxuan Zhou10Yueqi Ni11Jinglun Zhang12Hebin Liao13Jingwen Wu14Yufeng Zhang15State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan UniversityMedical Research Institute School of Medicine Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan UniversityAbstract Periodontitis, a leading cause of adult tooth loss and linked to various systemic diseases, is promoted by subgingival plaque biofilms, with Streptococci as early colonizers responsible for surface adhesion. Current studies of Streptococci adhesion have focused on bacteria surface adhesins with acquired protein membranes on the tooth surface, yet no critical proteins with implications for the overall early adhesion of subgingival plaque have been reported. Here, we identified that the “Barrel-like adhesion domain” of streptococcal EF-Tu facilitates cell-surface attachment, promotes biofilm formation, and contributes to the development of periodontitis. In the adherent state, EF-Tu is transported from the cytoplasm to the cell surface through membrane vesicles. Furthermore, we first found that simeprevir, an FDA-approved drug, binds to the “Barrel-like adhesion domain” of EF-Tu and effectively inhibits the protein’s surface adhesion and secretory pathways. Simeprevir showed the ability to inhibit dental plaque formation and provided prevention and treatments for periodontitis.https://doi.org/10.1038/s41522-025-00680-3 |
| spellingShingle | Leyi Xiao Yingying Pu Yu Cui Chen Chen Qi Xiao Yulan Wang Yan Wei Mengge Feng Tiange Zhang Shanyi Yang Jingxuan Zhou Yueqi Ni Jinglun Zhang Hebin Liao Jingwen Wu Yufeng Zhang Elongation factor Tu promotes the onset of periodontitis through mediating bacteria adhesion npj Biofilms and Microbiomes |
| title | Elongation factor Tu promotes the onset of periodontitis through mediating bacteria adhesion |
| title_full | Elongation factor Tu promotes the onset of periodontitis through mediating bacteria adhesion |
| title_fullStr | Elongation factor Tu promotes the onset of periodontitis through mediating bacteria adhesion |
| title_full_unstemmed | Elongation factor Tu promotes the onset of periodontitis through mediating bacteria adhesion |
| title_short | Elongation factor Tu promotes the onset of periodontitis through mediating bacteria adhesion |
| title_sort | elongation factor tu promotes the onset of periodontitis through mediating bacteria adhesion |
| url | https://doi.org/10.1038/s41522-025-00680-3 |
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