Protective Effect of Sika Deer (Cervus nippon) Velvet Antler Extract against Cisplatin-Induced Kidney and Liver Injury in a Prostate Cancer PC-3 Cell Xenograft Model
We previously discovered the antioxidant and antiprostate cancer effects of antler extract (AE), but whether it inhibits cisplatin- (Cis-) induced toxicity has not been investigated. In this study, the effect of AE on Cis-induced side effects in the kidney and liver using 3-(4,5-dimethyl-2-thiazolyl...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/6705156 |
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| author | Yujiao Tang Meiqi Fan Young-Jin Choi Eun-Ju Choi Sang-Ho Moon Trishna Debnath Yonghai Yu Il Nam Lee Eun-Kyung Kim |
| author_facet | Yujiao Tang Meiqi Fan Young-Jin Choi Eun-Ju Choi Sang-Ho Moon Trishna Debnath Yonghai Yu Il Nam Lee Eun-Kyung Kim |
| author_sort | Yujiao Tang |
| collection | DOAJ |
| description | We previously discovered the antioxidant and antiprostate cancer effects of antler extract (AE), but whether it inhibits cisplatin- (Cis-) induced toxicity has not been investigated. In this study, the effect of AE on Cis-induced side effects in the kidney and liver using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide-based cytotoxicity and cell cycle assays in prostate cancer PC-3 cells in vitro is investigated. Furthermore, we used a xenograft mouse model of the same cells to examine the in vivo effects and mechanisms of action. Cis and Cis + AE treatment attenuated prostate cancer cell growth by inducing apoptosis in vitro. Cis + AE stimulated cleaved caspases 3, 7, and 9 and polyadenosine diphosphate ribose polymerase expression. Cis + AE treatment for 1 week significantly increased the superoxide dismutase and catalase antioxidant activity while thiobarbituric acid reactive substances decreased. The histopathological damage and tumor necrosis factor-α, interleukin- (IL-) 1β and IL-6, cyclooxygenase-2, and inducible nitric oxide synthase expression in the kidney and liver tissue decreased. Therefore, AE likely possesses antiprostate cancer activity and inhibits Cis toxicity. |
| format | Article |
| id | doaj-art-ba71b5846a5a46ed9feb47cd20f9b1d1 |
| institution | OA Journals |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-ba71b5846a5a46ed9feb47cd20f9b1d12025-08-20T02:19:26ZengWileyJournal of Chemistry2090-90632090-90712018-01-01201810.1155/2018/67051566705156Protective Effect of Sika Deer (Cervus nippon) Velvet Antler Extract against Cisplatin-Induced Kidney and Liver Injury in a Prostate Cancer PC-3 Cell Xenograft ModelYujiao Tang0Meiqi Fan1Young-Jin Choi2Eun-Ju Choi3Sang-Ho Moon4Trishna Debnath5Yonghai Yu6Il Nam Lee7Eun-Kyung Kim8School of Bio-science and Food Engineering, Changchun University of Science and Technology, Changchun 130-600, ChinaDivision of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, Republic of KoreaDivision of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, Republic of KoreaDepartment of Physical Education, College of Education, Daegu Catholic University, Gyeongsan 38430, Republic of KoreaDivision of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, Republic of KoreaDepartment of Food Science and Biotechnology, Dongguk University, Goyang 10326, Republic of KoreaSchool of Bio-science and Food Engineering, Changchun University of Science and Technology, Changchun 130-600, ChinaR&D Department, Korea Mcnulty Co., Ltd., Cheonan 31009, Republic of KoreaDivision of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, Republic of KoreaWe previously discovered the antioxidant and antiprostate cancer effects of antler extract (AE), but whether it inhibits cisplatin- (Cis-) induced toxicity has not been investigated. In this study, the effect of AE on Cis-induced side effects in the kidney and liver using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide-based cytotoxicity and cell cycle assays in prostate cancer PC-3 cells in vitro is investigated. Furthermore, we used a xenograft mouse model of the same cells to examine the in vivo effects and mechanisms of action. Cis and Cis + AE treatment attenuated prostate cancer cell growth by inducing apoptosis in vitro. Cis + AE stimulated cleaved caspases 3, 7, and 9 and polyadenosine diphosphate ribose polymerase expression. Cis + AE treatment for 1 week significantly increased the superoxide dismutase and catalase antioxidant activity while thiobarbituric acid reactive substances decreased. The histopathological damage and tumor necrosis factor-α, interleukin- (IL-) 1β and IL-6, cyclooxygenase-2, and inducible nitric oxide synthase expression in the kidney and liver tissue decreased. Therefore, AE likely possesses antiprostate cancer activity and inhibits Cis toxicity.http://dx.doi.org/10.1155/2018/6705156 |
| spellingShingle | Yujiao Tang Meiqi Fan Young-Jin Choi Eun-Ju Choi Sang-Ho Moon Trishna Debnath Yonghai Yu Il Nam Lee Eun-Kyung Kim Protective Effect of Sika Deer (Cervus nippon) Velvet Antler Extract against Cisplatin-Induced Kidney and Liver Injury in a Prostate Cancer PC-3 Cell Xenograft Model Journal of Chemistry |
| title | Protective Effect of Sika Deer (Cervus nippon) Velvet Antler Extract against Cisplatin-Induced Kidney and Liver Injury in a Prostate Cancer PC-3 Cell Xenograft Model |
| title_full | Protective Effect of Sika Deer (Cervus nippon) Velvet Antler Extract against Cisplatin-Induced Kidney and Liver Injury in a Prostate Cancer PC-3 Cell Xenograft Model |
| title_fullStr | Protective Effect of Sika Deer (Cervus nippon) Velvet Antler Extract against Cisplatin-Induced Kidney and Liver Injury in a Prostate Cancer PC-3 Cell Xenograft Model |
| title_full_unstemmed | Protective Effect of Sika Deer (Cervus nippon) Velvet Antler Extract against Cisplatin-Induced Kidney and Liver Injury in a Prostate Cancer PC-3 Cell Xenograft Model |
| title_short | Protective Effect of Sika Deer (Cervus nippon) Velvet Antler Extract against Cisplatin-Induced Kidney and Liver Injury in a Prostate Cancer PC-3 Cell Xenograft Model |
| title_sort | protective effect of sika deer cervus nippon velvet antler extract against cisplatin induced kidney and liver injury in a prostate cancer pc 3 cell xenograft model |
| url | http://dx.doi.org/10.1155/2018/6705156 |
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