DNA repair protein XPA is differentially expressed in colorectal cancer and predicts better prognosis
Abstract As an indispensable factor in DNA damage recognition step of nucleotide excision repair, XPA interacts with a series of proteins to initiate repair process. The expression characteristics of XPA in colorectal cancer (CRC) and its influence on CRC prognosis remain elusive. Tissue specimens o...
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| Format: | Article |
| Language: | English |
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Wiley
2018-06-01
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| Series: | Cancer Medicine |
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| Online Access: | https://doi.org/10.1002/cam4.1480 |
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| author | Xue Feng Jingwei Liu Yuehua Gong Kaihua Gou Huaiwei Yang Yuan Yuan Chengzhong Xing |
| author_facet | Xue Feng Jingwei Liu Yuehua Gong Kaihua Gou Huaiwei Yang Yuan Yuan Chengzhong Xing |
| author_sort | Xue Feng |
| collection | DOAJ |
| description | Abstract As an indispensable factor in DNA damage recognition step of nucleotide excision repair, XPA interacts with a series of proteins to initiate repair process. The expression characteristics of XPA in colorectal cancer (CRC) and its influence on CRC prognosis remain elusive. Tissue specimens of CRC and nontumor adjacent tissues from 283 patients were collected. XPA protein expressions were detected by immunohistochemistry staining. Nonparametric test was used to investigate the difference of XPA expression between CRC and nontumor adjacent tissues, as well as the correlation between XPA expression and clinicopathological parameters of CRC. Univariate and multivariate Cox proportional hazards models were applied to estimate the relationship between XPA expression and CRC prognosis. Meanwhile, we analyzed TCGA data to investigate the relation between XPA mRNA expression and survival of CRC. XPA protein expression was significantly decreased in CRC tissues compared with nontumor adjacent tissues (P = 0.001). Subgroup analysis indicated consistently significant down‐regulation of XPA in CRC tissues in age > 60 (P = 0.026), age ≤ 60 (P = 0.008), colon cancer (P = 0.009), and rectal cancer (P = 0.015) patients and males (P = 0.004). For clinicopathological parameters, CRC patients with drinking habits revealed XPA overexpression than nondrinkers (P = 0.032). For prognosis, CRC patients with high XPA protein expression had longer overall survival (OS) (HR = 0.62, 95%CI: 0.39–0.97, P = 0.037). Stratified analysis suggested a better prognosis in relation to high XPA protein expression in patients over 60 years (adjusted HR = 0.48, P = 0.021), with rectal cancer (HR = 0.56, P = 0.037), without distant metastasis (HR = 0.58, P = 0.033), without tumor deposits (HR = 0.40, P = 0.006; adjusted HR = 0.44, P = 0.028), and with tumor diameter over 4 cm (HR = 0.49, P = 0.023). DNA repair protein XPA is significantly decreased in colorectal cancer tissues than in adjacent nontumor tissues. High expression of XPA protein showed significant relationship with better survival of CRC, especially rectal cancer. XPA might be a novel biomarker but might not be an independent factor to predict prognosis of CRC patients. |
| format | Article |
| id | doaj-art-ba6f7f031e424f0292ce9831b43f50cf |
| institution | OA Journals |
| issn | 2045-7634 |
| language | English |
| publishDate | 2018-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj-art-ba6f7f031e424f0292ce9831b43f50cf2025-08-20T02:38:17ZengWileyCancer Medicine2045-76342018-06-01762339234910.1002/cam4.1480DNA repair protein XPA is differentially expressed in colorectal cancer and predicts better prognosisXue Feng0Jingwei Liu1Yuehua Gong2Kaihua Gou3Huaiwei Yang4Yuan Yuan5Chengzhong Xing6Tumor Etiology and Screening Department of Cancer Institute and General Surgery The First Hospital of China Medical University Shenyang 110001 ChinaTumor Etiology and Screening Department of Cancer Institute and General Surgery The First Hospital of China Medical University Shenyang 110001 ChinaTumor Etiology and Screening Department of Cancer Institute and General Surgery The First Hospital of China Medical University Shenyang 110001 ChinaTumor Etiology and Screening Department of Cancer Institute and General Surgery The First Hospital of China Medical University Shenyang 110001 ChinaTumor Etiology and Screening Department of Cancer Institute and General Surgery The First Hospital of China Medical University Shenyang 110001 ChinaTumor Etiology and Screening Department of Cancer Institute and General Surgery The First Hospital of China Medical University Shenyang 110001 ChinaTumor Etiology and Screening Department of Cancer Institute and General Surgery The First Hospital of China Medical University Shenyang 110001 ChinaAbstract As an indispensable factor in DNA damage recognition step of nucleotide excision repair, XPA interacts with a series of proteins to initiate repair process. The expression characteristics of XPA in colorectal cancer (CRC) and its influence on CRC prognosis remain elusive. Tissue specimens of CRC and nontumor adjacent tissues from 283 patients were collected. XPA protein expressions were detected by immunohistochemistry staining. Nonparametric test was used to investigate the difference of XPA expression between CRC and nontumor adjacent tissues, as well as the correlation between XPA expression and clinicopathological parameters of CRC. Univariate and multivariate Cox proportional hazards models were applied to estimate the relationship between XPA expression and CRC prognosis. Meanwhile, we analyzed TCGA data to investigate the relation between XPA mRNA expression and survival of CRC. XPA protein expression was significantly decreased in CRC tissues compared with nontumor adjacent tissues (P = 0.001). Subgroup analysis indicated consistently significant down‐regulation of XPA in CRC tissues in age > 60 (P = 0.026), age ≤ 60 (P = 0.008), colon cancer (P = 0.009), and rectal cancer (P = 0.015) patients and males (P = 0.004). For clinicopathological parameters, CRC patients with drinking habits revealed XPA overexpression than nondrinkers (P = 0.032). For prognosis, CRC patients with high XPA protein expression had longer overall survival (OS) (HR = 0.62, 95%CI: 0.39–0.97, P = 0.037). Stratified analysis suggested a better prognosis in relation to high XPA protein expression in patients over 60 years (adjusted HR = 0.48, P = 0.021), with rectal cancer (HR = 0.56, P = 0.037), without distant metastasis (HR = 0.58, P = 0.033), without tumor deposits (HR = 0.40, P = 0.006; adjusted HR = 0.44, P = 0.028), and with tumor diameter over 4 cm (HR = 0.49, P = 0.023). DNA repair protein XPA is significantly decreased in colorectal cancer tissues than in adjacent nontumor tissues. High expression of XPA protein showed significant relationship with better survival of CRC, especially rectal cancer. XPA might be a novel biomarker but might not be an independent factor to predict prognosis of CRC patients.https://doi.org/10.1002/cam4.1480Colorectal cancerprognosisXPA |
| spellingShingle | Xue Feng Jingwei Liu Yuehua Gong Kaihua Gou Huaiwei Yang Yuan Yuan Chengzhong Xing DNA repair protein XPA is differentially expressed in colorectal cancer and predicts better prognosis Cancer Medicine Colorectal cancer prognosis XPA |
| title | DNA repair protein XPA is differentially expressed in colorectal cancer and predicts better prognosis |
| title_full | DNA repair protein XPA is differentially expressed in colorectal cancer and predicts better prognosis |
| title_fullStr | DNA repair protein XPA is differentially expressed in colorectal cancer and predicts better prognosis |
| title_full_unstemmed | DNA repair protein XPA is differentially expressed in colorectal cancer and predicts better prognosis |
| title_short | DNA repair protein XPA is differentially expressed in colorectal cancer and predicts better prognosis |
| title_sort | dna repair protein xpa is differentially expressed in colorectal cancer and predicts better prognosis |
| topic | Colorectal cancer prognosis XPA |
| url | https://doi.org/10.1002/cam4.1480 |
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