Floral reward traits change between sexual phases in two Helleborus species

Abstract Nectar and pollen attractants as well as visitors in Helleborus viridis ssp. viridis and H. purpurascens (Ranunculaceae), i.e. European species, were studied during two flowering seasons. The long-lived flowers of these species are characterized by long nectar availability lasting 15–17 day...

Full description

Saved in:
Bibliographic Details
Main Authors: Aneta Sulborska-Różycka, Bożena Denisow, Monika Strzałkowska-Abramek, Katarzyna Jaśkiewicz
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Reports
Subjects:
Online Access:https://doi.org/10.1038/s41598-025-96806-w
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850202496705757184
author Aneta Sulborska-Różycka
Bożena Denisow
Monika Strzałkowska-Abramek
Katarzyna Jaśkiewicz
author_facet Aneta Sulborska-Różycka
Bożena Denisow
Monika Strzałkowska-Abramek
Katarzyna Jaśkiewicz
author_sort Aneta Sulborska-Różycka
collection DOAJ
description Abstract Nectar and pollen attractants as well as visitors in Helleborus viridis ssp. viridis and H. purpurascens (Ranunculaceae), i.e. European species, were studied during two flowering seasons. The long-lived flowers of these species are characterized by long nectar availability lasting 15–17 days, i.e. until the end of pollen presentation. On average, approx. 2-fold more nectar and sugar mass was available in the flowers of H. viridis ssp. viridis compared to H. purpurascens. Both species are protogynous, having an initial female phase lasting for 3 days. The nectar and sugar mass was consistently male-biased. At the end of the flower’s life span, the nectar mass and the sugar concentration decreased considerably. In both species, sucrose predominated in the nectar, which also contained small quantities of other disaccharides (maltose and trehalose) and monosaccharides (glucose and fructose). The chemical composition of the nectar sugar varied between the sexual phases; sucrose in the female-phase nectar was replaced mainly by fructose. In addition to nectar, the flowers offered pollen (between 2 and 4 and 10–17 days of anthesis). In the study area, both Helleborus species were foraged by queens of bumble bees and honey bees; however, their distribution differed between the species. Bumble bees dominated in H. viridis subsp. viridis, whereas both pollinator groups were noted with a similar frequency in H. purpurascens.
format Article
id doaj-art-ba638d3d69b9481ca5de7468ca97b989
institution OA Journals
issn 2045-2322
language English
publishDate 2025-04-01
publisher Nature Portfolio
record_format Article
series Scientific Reports
spelling doaj-art-ba638d3d69b9481ca5de7468ca97b9892025-08-20T02:11:46ZengNature PortfolioScientific Reports2045-23222025-04-0115111510.1038/s41598-025-96806-wFloral reward traits change between sexual phases in two Helleborus speciesAneta Sulborska-Różycka0Bożena Denisow1Monika Strzałkowska-Abramek2Katarzyna Jaśkiewicz3Department of Botany and Plant Physiology, University of Life Sciences in LublinDepartment of Botany and Plant Physiology, University of Life Sciences in LublinDepartment of Botany and Plant Physiology, University of Life Sciences in LublinThe National Institute of Horticultural ResearchAbstract Nectar and pollen attractants as well as visitors in Helleborus viridis ssp. viridis and H. purpurascens (Ranunculaceae), i.e. European species, were studied during two flowering seasons. The long-lived flowers of these species are characterized by long nectar availability lasting 15–17 days, i.e. until the end of pollen presentation. On average, approx. 2-fold more nectar and sugar mass was available in the flowers of H. viridis ssp. viridis compared to H. purpurascens. Both species are protogynous, having an initial female phase lasting for 3 days. The nectar and sugar mass was consistently male-biased. At the end of the flower’s life span, the nectar mass and the sugar concentration decreased considerably. In both species, sucrose predominated in the nectar, which also contained small quantities of other disaccharides (maltose and trehalose) and monosaccharides (glucose and fructose). The chemical composition of the nectar sugar varied between the sexual phases; sucrose in the female-phase nectar was replaced mainly by fructose. In addition to nectar, the flowers offered pollen (between 2 and 4 and 10–17 days of anthesis). In the study area, both Helleborus species were foraged by queens of bumble bees and honey bees; however, their distribution differed between the species. Bumble bees dominated in H. viridis subsp. viridis, whereas both pollinator groups were noted with a similar frequency in H. purpurascens.https://doi.org/10.1038/s41598-025-96806-wMale-biased nectar productionSucrose predominance in nectarPollen productionPollen protein contentBumble bees
spellingShingle Aneta Sulborska-Różycka
Bożena Denisow
Monika Strzałkowska-Abramek
Katarzyna Jaśkiewicz
Floral reward traits change between sexual phases in two Helleborus species
Scientific Reports
Male-biased nectar production
Sucrose predominance in nectar
Pollen production
Pollen protein content
Bumble bees
title Floral reward traits change between sexual phases in two Helleborus species
title_full Floral reward traits change between sexual phases in two Helleborus species
title_fullStr Floral reward traits change between sexual phases in two Helleborus species
title_full_unstemmed Floral reward traits change between sexual phases in two Helleborus species
title_short Floral reward traits change between sexual phases in two Helleborus species
title_sort floral reward traits change between sexual phases in two helleborus species
topic Male-biased nectar production
Sucrose predominance in nectar
Pollen production
Pollen protein content
Bumble bees
url https://doi.org/10.1038/s41598-025-96806-w
work_keys_str_mv AT anetasulborskarozycka floralrewardtraitschangebetweensexualphasesintwohelleborusspecies
AT bozenadenisow floralrewardtraitschangebetweensexualphasesintwohelleborusspecies
AT monikastrzałkowskaabramek floralrewardtraitschangebetweensexualphasesintwohelleborusspecies
AT katarzynajaskiewicz floralrewardtraitschangebetweensexualphasesintwohelleborusspecies