Early functional and structural hippocampal impairment in a bilateral common carotid artery stenosis mouse model
Abstract Background Subcortical ischemic vascular dementia (SIVD) is a common subtype of vascular dementia. Currently, the bilateral common carotid artery stenosis (BCAS) mouse model is the most suitable SIVD rodent model. In this study, we investigated the functional and structural impairments in t...
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Wiley
2025-06-01
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| Series: | Animal Models and Experimental Medicine |
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| Online Access: | https://doi.org/10.1002/ame2.12549 |
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| author | Ping Tang Yi Sun Chunsheng Yang Nan Zhang |
| author_facet | Ping Tang Yi Sun Chunsheng Yang Nan Zhang |
| author_sort | Ping Tang |
| collection | DOAJ |
| description | Abstract Background Subcortical ischemic vascular dementia (SIVD) is a common subtype of vascular dementia. Currently, the bilateral common carotid artery stenosis (BCAS) mouse model is the most suitable SIVD rodent model. In this study, we investigated the functional and structural impairments in the hippocampus 1 month after BCAS. Methods We used behavioral tests, laser speckle flowmetry, long‐term potentiation, histochemical staining, molecular experiments, and voxel‐based morphometry to evaluate the hippocampal impairments. Results Behavioral studies revealed that BCAS mice exhibited worse performance. Laser speckle flowmetry detected an obvious decrease in cerebral blood flow. The synaptic plasticity of the perforant path‐dentate gyrus pathway was inhibited. Decreased fractional anisotropy and increased mean diffusivity were detected in the hippocampus via diffusion tensor imaging data. A reduction in gray matter volume, which was most prominent in the hippocampus and its surrounding areas, was detected via voxel‐based morphometry analysis. Impairments in cell morphology and myelin integrity were validated using histochemical staining and molecular biology techniques. In addition, the numbers of GFAP+ astrocytes and Iba1+ microglia increased in the hippocampus. Conclusions Overall, our study demonstrates early functional and structural impairments in the hippocampus contributing to learning and memory deficits after 1 month of BCAS, indicating that the hippocampus is vulnerable to chronic cerebral ischemia. |
| format | Article |
| id | doaj-art-ba5cf03379bd4d40ac0eaae6dd25335f |
| institution | DOAJ |
| issn | 2576-2095 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
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| series | Animal Models and Experimental Medicine |
| spelling | doaj-art-ba5cf03379bd4d40ac0eaae6dd25335f2025-08-20T03:24:04ZengWileyAnimal Models and Experimental Medicine2576-20952025-06-01861033104310.1002/ame2.12549Early functional and structural hippocampal impairment in a bilateral common carotid artery stenosis mouse modelPing Tang0Yi Sun1Chunsheng Yang2Nan Zhang3Department of Neurology Tianjin Neurological Institute, Tianjin Medical University General Hospital Tianjin ChinaDepartment of Neurology Tianjin Neurological Institute, Tianjin Medical University General Hospital Tianjin ChinaDepartment of Neurology Tianjin Neurological Institute, Tianjin Medical University General Hospital Tianjin ChinaDepartment of Neurology Tianjin Neurological Institute, Tianjin Medical University General Hospital Tianjin ChinaAbstract Background Subcortical ischemic vascular dementia (SIVD) is a common subtype of vascular dementia. Currently, the bilateral common carotid artery stenosis (BCAS) mouse model is the most suitable SIVD rodent model. In this study, we investigated the functional and structural impairments in the hippocampus 1 month after BCAS. Methods We used behavioral tests, laser speckle flowmetry, long‐term potentiation, histochemical staining, molecular experiments, and voxel‐based morphometry to evaluate the hippocampal impairments. Results Behavioral studies revealed that BCAS mice exhibited worse performance. Laser speckle flowmetry detected an obvious decrease in cerebral blood flow. The synaptic plasticity of the perforant path‐dentate gyrus pathway was inhibited. Decreased fractional anisotropy and increased mean diffusivity were detected in the hippocampus via diffusion tensor imaging data. A reduction in gray matter volume, which was most prominent in the hippocampus and its surrounding areas, was detected via voxel‐based morphometry analysis. Impairments in cell morphology and myelin integrity were validated using histochemical staining and molecular biology techniques. In addition, the numbers of GFAP+ astrocytes and Iba1+ microglia increased in the hippocampus. Conclusions Overall, our study demonstrates early functional and structural impairments in the hippocampus contributing to learning and memory deficits after 1 month of BCAS, indicating that the hippocampus is vulnerable to chronic cerebral ischemia.https://doi.org/10.1002/ame2.12549bilateral carotid artery stenosis modelhippocampusmagnetic resonance imagingsubcortical ischemic vascular dementiavoxel‐based morphometry |
| spellingShingle | Ping Tang Yi Sun Chunsheng Yang Nan Zhang Early functional and structural hippocampal impairment in a bilateral common carotid artery stenosis mouse model Animal Models and Experimental Medicine bilateral carotid artery stenosis model hippocampus magnetic resonance imaging subcortical ischemic vascular dementia voxel‐based morphometry |
| title | Early functional and structural hippocampal impairment in a bilateral common carotid artery stenosis mouse model |
| title_full | Early functional and structural hippocampal impairment in a bilateral common carotid artery stenosis mouse model |
| title_fullStr | Early functional and structural hippocampal impairment in a bilateral common carotid artery stenosis mouse model |
| title_full_unstemmed | Early functional and structural hippocampal impairment in a bilateral common carotid artery stenosis mouse model |
| title_short | Early functional and structural hippocampal impairment in a bilateral common carotid artery stenosis mouse model |
| title_sort | early functional and structural hippocampal impairment in a bilateral common carotid artery stenosis mouse model |
| topic | bilateral carotid artery stenosis model hippocampus magnetic resonance imaging subcortical ischemic vascular dementia voxel‐based morphometry |
| url | https://doi.org/10.1002/ame2.12549 |
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